Effect of dietary zinc pectin oligosaccharides chelate on growth performance, enzyme activities, Zn accumulation, metallothionein concentration, and gene expression of Zn transporters in broiler chickens1

Abstract

This study was to investigate the effect of zinc pectin oligosaccharides chelate (Zn-POS) on growth performance, serum enzyme activities, tissue zinc accumulation, metallothionein (MT) concentrations, and gene expression of zinc transporters (ZnT) in broilers. Five hundred forty 1-d-old Arbor Acres broiler chicks were randomly assigned to 5 dietary groups with 6 replicates of 18 birds per replicate. The diets were formulated with the same supplemental Zn level (80 mg/kg diet) but different amount of the Zn-POS: 0, 200, 400, 600, and 800 mg Zn-POS/kg diet. ZnSO 4 was used to adjust to the desired amount of the Zn (80 mg/kg) in the Zn-POS diets. Broilers were fed with the experimental diets for 42 d including the starter (days 1 to 21) and grower (days 22 to 42) phases. Our results showed that dietary supplementation of Zn-POS linearly and quadratically increased (P < 0.05) the average daily gain and gain-tofeed ratio during 22 to 42 d and 1 to 42 d as well as body weight on day 42, whereas reduced (P < 0.05) the sum of mortality and lag abnormalities in broilers on day 42. Besides, serum alkaline phosphatase and copper-zinc superoxide dismutase activities increased (P < 0.05) linearly and quadratically in response to dietary Zn-POS supplemental level on day 42. Dietary Zn-POS supplementation increased Zn accumulation in serum (linear, P < 0.05), liver (linear, P < 0.05), and pancreas (linear and quadratic, P < 0.05). In addition, Zn-POS supplementation linearly and quadratically increased (P < 0.01, P < 0.05, respectively) MT concentrations in liver and pancreas of broilers. Pancreatic mRNA levels of MT, ZnT-1, and ZnT-2 increased (P < 0.05) linearly and quadratically, and the mRNA expression of metal response element-binding transcription factor-1 increased linearly (P < 0.05), in response to dietary Zn-POS supplementation. In conclusion, supplementation of Zn-POS in the diet increases Zn enrichment in the metabolic organs such as liver and pancreas and promotes productive performance in broilers.

Interplay between zinc and cell proliferation and implications for the growth of livestock

“…Dietary supplemented with 100 mg/kg ZnSO4 or glycine‐Zn could improve the growth performance and gut barrier function of weaned piglets, and organic Zn had the best effect (Diao et al, 2021). The outcomes from broilers showed that dietary supplementation of 0, 200, 400, 600 and 800 mg/kg Zn pectin oligosaccharides chelate linearly and quadratically increased the body weight on d 42 and the average daily gain and gain‐to‐feed ratio during 22–42 days and 1–42 days (Wang et al, 2019). Compared to broilers fed conventional Zn sources (60 mg/kg), broilers given dose of nano‐Zn (20 mg/kg) performed better in terms of growth (Zhao et al, 2014).…”

Effects of Zinc Glycinate on Growth Performance, Serum Biochemical Indexes, and Intestinal Morphology of Yellow Feather Broilers

“…Zinc can effectively regulate MT gene expression at the transcriptional level [44][45][46]. Wang et al [47] found that MT concentrations in the liver and pancreas of broilers supplemented with organic zinc were significantly higher than those in the control group. Different zinc sources also have an important effect on the expression of MT mRNA.…”

Effects of Dietary Zinc Supplements on the Antioxidant Indicators and the Expression of Zinc Transport Genes in Korean Native Chicks

“…흡수된 아연 은 소장 점막세포 원형질액에서 MT 단백질과 함께 결합하여 간으로 이동되거나 소포체에 저장된다(Nishito and Kambe, 2018). MT는 소장, 간 등 여러 장기에 존재하는 아연 수용 체로서 시스테인 함량이 23∼33%인 thiol성 단백질로 활성 ZnT-5와 MT 단백질 mRNA 수준은 아연의 급여원에 따라 이들 유전자가 다르게 발현될 수 있음을 보고하였다 Wang et al(2019)…”