Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

Abstract

While a high-fat diet (HFD) is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity, probiotics are believed to have positive effects on the regulation of HFD-induced obesity as well as lipid metabolism, energy homeostasis, and anti-oxidation. Because Bacillus subtilis B10 has beneficial effects on the abnormal lipid metabolism and the oxidative stress in HFD-induced obese mice, ICR mice were randomly assigned into an HFD group and the HFD was supplemented with 0.1% (w/w) Bacillus subtilis B10 (HFD+B10 group). Thereafter, 30-d treatments were run, and then hepatic lipid level and antioxidant status were measured. The expression of genes related to lipid metabolism and oxidative stress in the liver was determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR). We found that HFD-induced obese mice treated with B10 showed a decrease in weight gain, serum glucose activity as well as hepatic triglyceride (TG), glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) activities. In addition, the gene expressions of antioxidant genes, glutathione reductase (GR), xanthine oxidase (XO), heat-shock protein 90 (Hsp90), and lipid synthesis gene 3β-hydroxysteroid-∆24 reductase (DHCR24) in the HFD+B10 group were down-regulated, suggesting alleviation of oxidative stress, while the lipolysis gene 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), energy metabolism gene peroxisome proliferator-activated receptor α (PPARα) and the gene encoding tumor-suppressor protein p53 were up-regulated. The regulatory and positive effect of dietary supplementation of probiotic B10 suggests that it has a beneficial effect on the homeostasis of the lipid metabolism and on alleviating oxidative stress in HFD-induced obese mice.

Myricetin protects against diet-induced obesity and ameliorates oxidative stress in C57BL/6 mice

“…Real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed as described by Wu et al (2014) and Lei et al (2015). Total RNA was isolated from the epididymal adipose tissue of each mouse using TRIzol (Invitrogen) and then pooled for the RT-PCR analysis.…”

Effects of chromium-enriched bacillus subtilis KT260179 supplementation on chicken growth performance, plasma lipid parameters, tissue chromium levels, cecal bacterial composition and breast meat quality

“…Oral administration of B. subtilis can exert a range of beneficial effects, including improvement of growth, enhancement of meat characteristics, optimizing the balance of intestinal microbiota, prevention and treatment of some diarrheal diseases, and reduction of serum cholesterol [9, 10]. For these reasons, B. subtilis has attracted considerable attention as a potentially beneficial dietary supplement for animal health.…”

Effects of chromium-enriched Bacillus subtilis KT260179 supplementation on growth performance, caecal microbiology, tissue chromium level, insulin receptor expression and plasma biochemical profile of mice under heat stress

“…Bacillus subtilis is a probiotic bacterium that is widely used in diets of both humans and animals (16,17) . Oral administration of B. subtilis can exert a range of beneficial effects including optimising the balance of intestinal microbiota, prevention and treatment of some diarrhoeal diseases, improvement of growth, enhancement of immune responses and reduction of serum cholesterol levels (18)(19)(20) . For these reasons, B. subtilis has attracted considerable attention as a potentially beneficial dietary supplement for human and animal health.…”