Effect of dietary Astragalus Polysaccharide supplements on testicular miRNA expression profiles and enzymatic changes of breeder cocks

Abstract

Astragalus Polysaccharide (APS) is an important feed additive due to its immunomodulatory functions. Previous studies have proven that miRNAs play important roles in posttranscriptional gene regulation. Our goals were to identify differentially expressed miRNAs in testes in responses to APS dietary supplements and to find the effects of APS on breeder cock testes. We measured several enzymatic activities in testes and sperm samples and further generated miRNA expression profiles of testes from breeder cocks fed with control diets and extra APS. As a result, we found APS could increase testicular functional activities of marker enzymes. Meanwhile, there were 16 up-regulated and 17 down-regulated miRNAs in APS group, compared with the control group meeting the criteria of P-values < 0.05. Meanwhile, twelve differentially expressed miRNAs were validated by Mir-XTM miRNA RT-qPCR. Further GO and KEGG analyses of target genes for differentially expressed miRNAs revealed that some miRNAs may be involved in testicular nutrient metabolisms and NK cell mediated cytotoxicity pathway. Moreover, the effect of dietary APS supplements on NK cell mediated cytotoxicity pathway was also validated by RT-qPCR. Our results provided a novel insight into the effect of dietary APS supplements on testicular miRNA expression profiles and enzymatic changes of breeder cocks.

Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens

“…(h) 3 0 untranslated region luciferase reporter assays and PC-3p-448507_5 mimic or miR-NTC (RIBO Bio, Guangzhou, China) using 2.0 µl X-tremeGENE siRNA Transfection Reagent (Roche, USA) into HEK-293 T cells, the expression levels of PC-5p-30232_97 and PC-3p-448507_5 in cells were measured by quantitative real time-PCR (qRT-PCR) and then luciferase reporter experiments were performed [19].…”

Astragalus polysaccharide attenuates metabolic memory-triggered ER stress and apoptosis via regulation of miR-204/SIRT1 axis in retinal pigment epithelial cells

“…APS suppressed miR-721 expression to attenuate TNF-α-induced insulin resistance in 3T3-L1 adipocytes [30]. Dietary APS supplements could regulate testicular miRNA expression profiles [39]. But, none of these studies have explicitly demonstrated whether APS modulated miRNA expression by directly participating in the transcription, or indirectly by regulating miRNA sponges.…”

Peer Review #3 of "Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFV in vitro (v0.1)"

“…33 the inhibitory effects of APS on porcine viruses infection have verified 34 and APS has been observed to regulate the levels of immunomodulating compounds in many different cell types. 35,36 The manifestation of adhesion molecules, including P-selectin and E-selectin, was inhibited by APS and persisted while the ECs were stimulated with CSFV, which could promote the expression of both P-and E-selectin ( Figure 2AB). Similarly, the increase of TF ( Figure 2D), TGF-β ( Figure 4C), and IL-8 ( Figure 3F) production in response to CSFV was inhibited by APS.…”