Effect of dietary ARA/EPA/DHA ratios on growth performance and intermediary metabolism of gilthead sea bream (Sparus aurata) juveniles

Magalhães, Rui; Guerreiro, Inês; Coutinho, Filipe; Moutinho, Sara; Sousa, Sara; Delerue–Matos, Cristina; Domingues, Valentina F.; Olsen, Rolf Erik; Perés, Helena; Oliva-Teles, Aires

doi:10.1016/j.aquaculture.2019.734644

Cited by 29 publications

(29 citation statements)

References 60 publications

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“…Indeed, a positive correlation between LC-PUFA content in different tissues and lipid peroxidation was already demonstrated in several fish species 14 , 29 , 31 , 42 . Even though liver and intestine FA profiles were not determined in the present study, muscle EPA and particularly DHA contents were higher in fish fed diets C and D than the other diets, while ARA content showed only slight variations 41 and it is expected that the FA concentrations in liver and intestine should follow a similar pattern. Therefore, a lower concentration of EPA and DHA might explain the lower LPO values in ARA rich diets.…”

Section: Discussioncontrasting

confidence: 64%

“…4 SIMPER, similarity percentage within group replicates. www.nature.com/scientificreports/ not determined in the present study, muscle EPA and particularly DHA contents were higher in fish fed diets C and D than the other diets, while ARA content showed only slight variations 41 and it is expected that the FA concentrations in liver and intestine should follow a similar pattern. Therefore, a lower concentration of EPA and DHA might explain the lower LPO values in ARA rich diets.…”

Section: Discussioncontrasting

confidence: 55%

“…Previously, Magalhães et al 41 showed that a diet with a balanced ARA/EPA/DHA (1/0.4/0.4) ratio that closely matches the estimated EFA requirements of gilthead seabream increased feed efficiency and protein efficiency ratio compared to diets with unbalanced ARA/EPA/DHA ratios (0/0.6/0.6 and 0/0.3/1.5). Dietary ARA/EPA/DHA ratios also affected lipid metabolism, with increased β-oxidation activity in fish fed high ARA/EPA/DHA (2.0/0.2/0.1) and decreased lipogenesis in fish fed high DHA (0/0.3/1.5).…”

Section: Introductionmentioning

confidence: 82%

“…Four plant-based diets were formulated as described in Magalhães et al 41 . Shortly, the experimental diets were isoproteic (47% crude protein; 74% protein from plant feedstuffs and 26% protein from fish meal) and isolipidic (18% crude lipids) with a vegetable oil blend (20:50:30 rapeseed, linseed, and palm oils) as main lipid source.…”

Section: Methodsmentioning

confidence: 99%

“…× 0.25 µm film thickness; Supelco, Bellefonte, USA). FA were identified by comparison with known standard mixtures as described by Magalhães et al 41 . www.nature.com/scientificreports/ Enzyme activity.…”

Section: Samplingmentioning

confidence: 99%

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Oxidative status and intestinal health of gilthead sea bream (Sparus aurata) juveniles fed diets with different ARA/EPA/DHA ratios

Magalhães

Guerreiro

Santos

et al. 2020

Sci Rep

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The present work assessed the effects of dietary ratios of essential fatty acids, arachidonic (ARA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), on liver and intestine oxidative status, intestinal histomorphology and gut microbiota of gilthead sea bream. Four isoproteic and isolipidic plant-based diets were formulated containing a vegetable oil blend as the main lipid source. Diets were supplemented with ARA/EPA/DHA levels (%DM) equivalent to:

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Section: Discussioncontrasting

confidence: 64%

Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Samplingmentioning

confidence: 99%

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Oxidative status and intestinal health of gilthead sea bream (Sparus aurata) juveniles fed diets with different ARA/EPA/DHA ratios

Magalhães

Guerreiro

Santos

et al. 2020

Sci Rep

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Essential Fatty Acids—‘Fueling Versus Controlling’

Steinberg

2022

Aquatic Animal Nutrition

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Antioxidant capacity and lipid composition of Brachionus plicatilis and Artemia enriched with a mixture of different post-processing formats of Navicula salinicola and Isochrysis galbana and lipid emulsions

Galindo,

Pérez,

Almansa

et al. 2024

J Appl Phycol

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Live prey used by the aquaculture industry are usually poor in some essential nutrients including long chain polyunsaturated fatty acids (LC-PUFA) and must be enriched to improve their nutritional value prior to larval feeding. Standard enrichment protocols are commonly based on lipid emulsions, being associated to a high oxidative stress condition. The combination of microalgae and lipid emulsion can palliate this situation, where the oxidative stress can be partially compensated by the antioxidant compounds present in microalgae. The maintenance of living microalgae in culture facilities is laborious, and the produced biomass may present fluctuating properties, leading to a serious bottleneck in the cultivation of live prey. Hence, substitutes for live microalgae including pastes or dried formats are receiving increasing research attention due to its nutritional stability, longer shelf-life and easy handling. In this study four different microalgae formats combined with a lipid emulsion are tested as enrichment products for Brachionus plicatilis and Artemia. Thus, fresh, frozen and spray-dried Navicula salinicola (NFRE, NFRO and NSD, respectively), and spray-dried Isochrysis galbana (ISD) were mixed with a commercial oil concentrate (IncromegaTM) or a marine lecithin (LC 60®), and added for 5 h to the rotifer or Artemia culture media. The antioxidant capacity of the microalgae extracts and the live prey activity of antioxidant enzymes, peroxides index (PxI) and thiobarbituric acid reactive substances (TBARS) were evaluated. The lipid profile of microalgae formats and enriched live preys was also determined. Ethyl acetate extract was the most antioxidant active extract of all microalgae formats. In addition, overall, I. galbana seems to be better than any N. salinicola format for a more effective protection against oxidative stress and for live prey lipid enrichment. Both rotifer and Artemia cultured with the mixture of I. galbana and the lipid emulsion generally showed higher DHA/EPA and EPA/ARA ratios. Moreover, the combination of the microalgae with LC 60® lipid emulsion highly favored Artemia´s polar lipid and DHA incorporation. Among microalgae products, both spray-dried formats better enhanced live prey n-3 LC-PUFA content. Our results highlight the great potential of new microalgae-derived products to improve effectiveness of current live prey lipid enrichment protocols used in aquaculture.

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Effect of dietary ARA/EPA/DHA ratios on growth performance and intermediary metabolism of gilthead sea bream (Sparus aurata) juveniles

Cited by 29 publications

References 60 publications

Oxidative status and intestinal health of gilthead sea bream (Sparus aurata) juveniles fed diets with different ARA/EPA/DHA ratios

Oxidative status and intestinal health of gilthead sea bream (Sparus aurata) juveniles fed diets with different ARA/EPA/DHA ratios

Essential Fatty Acids—‘Fueling Versus Controlling’

Antioxidant capacity and lipid composition of Brachionus plicatilis and Artemia enriched with a mixture of different post-processing formats of Navicula salinicola and Isochrysis galbana and lipid emulsions

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