Oxidative status and intestinal health of gilthead sea bream (Sparus aurata) juveniles fed diets with different ARA/EPA/DHA ratios

Magalhães, Rui; Guerreiro, Inês; Santos, Rafaela; Coutinho, Filipe; Couto, Ana; Serra, Cláudia R.; Olsen, Rolf Erik; Perés, Helena; Oliva-Teles, Aires

doi:10.1038/s41598-020-70716-5

Cited by 27 publications

(15 citation statements)

References 92 publications

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“…Sections were prepared at 5 μm using a Microm HM 315 rotary microtome (Microm International GmbH, Walldorf, Germany) and stained using Harris hematoxylin and eosin as described by Fischer et al [ 65 ]. Blind evaluation of histological preparation was performed, analyzing intestinal villi length [ 66 , 67 ]. Images were acquired with wave image software were processed, and length was measured using ImageJ1.53c software.…”

Section: Methodsmentioning

confidence: 99%

Reversal of Doxorubicin-Induced Bone Loss and Mineralization by Supplementation of Resveratrol and MitoTEMPO in the Early Development of Sparus aurata

et al. 2022

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Doxorubicin is a widely used chemotherapeutic drug known to induce bone loss. The mechanism behind doxorubicin-mediated bone loss is unclear, but oxidative stress has been suggested as a potential cause. Antioxidants that can counteract the toxic effect of doxorubicin on the bone would be helpful for the prevention of secondary osteoporosis. We used resveratrol, a natural antioxidant, and MitoTEMPO, a mitochondria-targeted antioxidant, to counteract doxorubicin-induced bone loss and mineralization on Sparus aurata larvae. Doxorubicin supplemented Microdiets increased bone deformities, decreased mineralization, and lipid peroxidation, whereas Resveratrol and MitoTEMPO supplemented microdiets improved mineralization, decreased bone deformities, and reversed the effects of doxorubicin in vivo and in vitro, using osteoblastic VSa13 cells. Partial Least-Squares Discriminant Analysis highlighted differences between groups on the distribution of skeletal anomalies and mineralization of skeleton elements. Calcium and Phosphorus content was negatively affected in the doxorubicin supplemented group. Doxorubicin reduced the mRNA expression of antioxidant genes, including catalase, glutathione peroxidase 1, superoxide dismutase 1, and hsp90 suggesting that ROS are central for Doxorubicin-induced bone loss. The mRNA expression of antioxidant genes was significantly increased on resveratrol alone or combined treatment. The length of intestinal villi was increased in response to antioxidants and reduced on doxorubicin. Antioxidant supplements effectively prevent bone deformities and mineralization defects, increase antioxidant response and reverse doxorubicin-induced effects on bone anomalies, mineralization, and oxidative stress. A combined treatment of doxorubicin and antioxidants was beneficial in fish larvae and showed the potential for use in preventing Doxorubicin-induced bone impairment.

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Section: Methodsmentioning

confidence: 99%

Reversal of Doxorubicin-Induced Bone Loss and Mineralization by Supplementation of Resveratrol and MitoTEMPO in the Early Development of Sparus aurata

et al. 2022

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“…Bradyrhizobium is believed to have antagonistic effects against 10 pathogenic bacteria ( Gabriella et al, 2017 ). Methanobacterium and Asinibacterium are considered to produce poly-β-hydroxybutyrates that inhibit the growth of pathogens in the host bacterial community ( Defoirdt et al, 2007 ; Halet et al, 2007 ; Magalhes et al, 2020 ). And, Variovorax paradoxus is associated with biofilm formation ( Fredendall et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Heat Stress Weakens the Skin Barrier Function in Sturgeon by Decreasing Mucus Secretion and Disrupting the Mucosal Microbiota

Yang

Tan

et al. 2022

Front. Microbiol.

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Heat stress induced by global warming has damaged the well-being of aquatic animals. The skin tissue plays a crucial role as a defense barrier to protect organism, however, little is known about the effect of heat stress on fish skin, particularly in cold-water fish species. Here, we investigated the effects of mild heat stress (24°C, MS) and high heat stress (28°C, HS) on Siberian sturgeon skin using RNA-seq, histological observation, and microbial diversity analysis. In RNA-seq, 8,819 differentially expressed genes (DEGs) in MS vs. C group and 12,814 DEGs in HS vs. C group were acquired, of which the MS vs. C and HS vs. C groups shared 3,903 DEGs, but only 1,652 DEGs were successfully annotated. The shared DEGs were significantly enriched in pathways associating with mucins synthesis. Histological observation showed that the heat stresses significantly reduced the number of skin mucous cells and induced the damages of epidermis. The microbial diversity analysis elicited that heat stress markedly disrupted the diversity and abundance of skin microbiota by increasing of potential pathogens (Vibrionimonas, Mesorhizobium, and Phyllobacterium) and decreasing of probiotics (Bradyrhizobium and Methylovirgula). In conclusion, this study reveals that heat stress causes adverse effects on sturgeon skin, reflecting in decreasing the mucus secretion and disordering the mucosal microbiota, which may contribute to develop the preventive strategy for heat stress caused by global warming.

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“…and NADPH as the main reducing power. However, fish produce proportionally higher rates of ROS, have lower activities of antioxidant enzymes and present more PUFA in cell membranes, which boost lipid peroxidation [ 2 , 8 ]. Therefore, the evaluation of the redox balance is relevant to establish the physiological status of fish.…”

Section: Discussionmentioning

confidence: 99%

“…Marine fish feed is rich in polyunsaturated fatty acids (PUFAs), which accumulate in tissues and provide a healthy meal for human consumption. However, their chemical structure makes them more susceptible to peroxidation, enhancing the prooxidant state [ 4 , 8 ]. At the same time, the level of lipids in feed modulates the activity of redox enzymes such as superoxide dismutase (SOD) and glutathione-related ones [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Interaction between the Effects of Sustained Swimming Activity and Dietary Macronutrient Proportions on the Redox Status of Gilthead Sea Bream Juveniles (Sparus aurata L.)

et al. 2022

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The combination of physical exercise and a balanced diet presents substantial health benefits and could improve fish production. However, the redox balance can be affected by training regimen, dietary macronutrient ratio and their interaction. In this study, we conjointly evaluated the effects of physical activity (by voluntary swimming (VS) or sustained swimming as exercise (Ex)) and diet composition (by high-protein (HP) or high-lipid (HE) commercial diets) after 6 weeks on oxidative stress status in liver, white muscle and red muscle of gilthead sea bream juveniles. The HE diet increased the biochemical redox markers’ thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPP) and reduced thiols (-SH) in the different tissues. Exercise increased AOPP and –SH levels in liver but reduced TBARS levels in white muscle. Regarding the expression of oxidative stress, chaperones and apoptosis-related genes, the VSHE group showed the highest values and the VSHP the lowest, whereas the application of sustained swimming partially equalized those differences. Diet composition modulated the enzyme activity, prioritizing the superoxide dismutase and catalase in the HE-fed groups and the glutathione-related enzymes in the HP groups. Exercise also altered enzyme activity, but in a tissue-dependent manner. Overall, the redox balance in gilthead sea bream juveniles can be affected by diet composition and sustained swimming. However, the response will partly depend on the interaction between these factors and the tissue studied. Therefore, the combination of an adequate diet and sustained exercise could be used in fish production to improve the physiological redox status.

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Oxidative status and intestinal health of gilthead sea bream (Sparus aurata) juveniles fed diets with different ARA/EPA/DHA ratios

Cited by 27 publications

References 92 publications

Reversal of Doxorubicin-Induced Bone Loss and Mineralization by Supplementation of Resveratrol and MitoTEMPO in the Early Development of Sparus aurata

Reversal of Doxorubicin-Induced Bone Loss and Mineralization by Supplementation of Resveratrol and MitoTEMPO in the Early Development of Sparus aurata

Heat Stress Weakens the Skin Barrier Function in Sturgeon by Decreasing Mucus Secretion and Disrupting the Mucosal Microbiota

Interaction between the Effects of Sustained Swimming Activity and Dietary Macronutrient Proportions on the Redox Status of Gilthead Sea Bream Juveniles (Sparus aurata L.)

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