Effect of dicyclohexylcarbodiimide on the proton conductance of thylakoid membranes in intact chloroplast

Булычев, А. А.; Андрианов, В. К.; Kurella, G. A.

doi:10.1016/0005-2728(80)90201-7

Cited by 19 publications

(3 citation statements)

References 14 publications

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“…Effect of uncouplers and electron transport inhibitors. Dicyclohexylcarbodiimide and oligomycin, both known to be very effective inhibitors of the ATPase in both photosynthetic and nonphotosynthetic organisms (3,25,26), were tested for their effects on transport. At concentrations of 167 pg/ml and 160 ,uM, respectively, no effect on the rate of transport was observed at either high (saturating) or low (limiting) light intensity.…”

Section: Resultsmentioning

confidence: 99%

Efficiency of light-driven metabolite transport in the photosynthetic bacterium Rhodospirillum rubrum

Zebrower¹,

Loach

1982

J Bacteriol

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An evaluation of the efficiency of the L-alanine and L-malate transport systems was undertaken with the photosynthetic bacterium Rhodospirillum rubrum grown on the amino acid whose uptake was measured. An all-glass apparatus was constructed for measuring transport activity under anaerobic conditions. L-Alanine transport activity decreased under conditions of Mg2e depletion. When cells were allowed to become inactive by suspending them in the dark in Mg2free buffer, full activity could be restored within a few minutes by adding 20 mM Mg2+ and illuminating the cells. The transport activity was completely inhibited by carbonyl cyanide m-trifluoromethoxyphenylhydrazone and by ammonia. The quantum yield for the uptake of either L-alanine or L-malate was 0.015 molecules per photon. The results are discussed in relation to the expected efficiencies for metabolite transport and regulation by Mg2+.

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Section: Resultsmentioning

confidence: 99%

Efficiency of light-driven metabolite transport in the photosynthetic bacterium Rhodospirillum rubrum

Zebrower¹,

Loach

1982

J Bacteriol

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“…The quasi steady-state potential in the light is determined by the balance of the light-driven electrogenic proton current and the passive ion currents through the membrane. The transient undershoot in the rapid light-off reaction has been interpreted to be caused by the negative diffusion potential associated with the proton gradient created in the preceding light period (Vredenberg 1976, Bulychev et al, 1980, This interpretation is corroborated by the observation that nigericin prevents the formation of the undershoot (Fig, IB), The dark relaxation of the undershoot in Fig, iA results from the passive dissipation of the proton gradient and has a half-time of about 5 s. The rate of linear electron transport was substantially increased in the presence of nigericin as reflected by the higher steady-stale potential in the light. This stimulation is likely caused by the removal of photosynthetic control at the quinol oxidation site Q,, of the cyt bjf complex (Bendall 1982, Weis et al, 1990) and at the PSD site (Foyer et al, 1990, Horton and Ruban 1992, Ehrenheim etal, 1994, Figure !C,D shows the inhibition of the light-induced potential by DCMU and the release of inhibition after adding TMPD, respectively.…”

Section: Methodsmentioning

confidence: 99%

Suppression of flash-induced PSII-dependent electrogenesis caused by proton pumping in chloroplasts

Voorthuysen¹,

Булычев²,

Dassen³

et al. 1996

Physiol Plant

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Suppression of fla,sh-induced PSD-dependent electrogenesis caused hy proton pumping in chloroplasts, -Physio], Plant, 98: 156-164,Pre-illumination of the thyiakoid membrane of Peperomia meiallica chloropiasts leads to a reversible suppression of the flash-induced electrical potential as measured either with the eiectrochromic bandshift (P515), microclectrode impalement or patchclamp technique. The energization-dependent potential suppression was nol obseived in the presence of 1 uM nigericin suggesting tiie involvement of proton and/or cation gradients. Energization in the presence of 3-(3,4-dichlorophenyl)-l,l-dimethylurea (;DCMU) and N,,N,N',N'-tetramethylphenylenediamine (TMPD), i,e, cyclic electron flow around photosystem (PS) 1, results in the accumulation of TMPD* in the thyiakoid lumen. The reversible suppression of the flash-induced tnembrane potential was nol observed in these conditions indicating that it is nol a general cation-induced increase of membrane capacitance. Cyclic electron flow around PSI in the presence of DCMU and phenazine methosulfate (PMS) results in the accumulation of P,MS' and H* in the thyiakoid lumen. The absence of reversible suppression of the flash-induced membrane potential for this condition shows that accumulation of protons does not lead to (I) a reversible increase of memhrane capacitance and (2) a reversible suppression of PSI-dependent electrogenesis. Reversible inactivation of PSIl by a low pH in the thyiakoid lumen is therefore proposed to be the cause for the lemporaiy suppression of the flash-induced electrical potential. The flash-induced PSII-dependent membrane potential, as measured after major oxidation of P700 in fai-red background light, was indeed found to be suppressed at low assay pH (pH 5) in isolated spinach {Spinacia oleracea} chioroplasts.

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“…However, for actinic illuminations of longer duration (~> 10 ms), the absorbance change is obscured by scattering changes and this technique is not applicable [38,12]. The only other kinetic technique available to measure the electric potential changes with sufficient time resolution, during actinic illumination periods of intermediate duration, is the open ended glass capillary microelectrode technique [6][7][8][9][10][11][49][50][51][52]. Recent results, comparing flash-induced potential changes measured with both of the above mentioned techniques in the same specimen, further enhance our trust in the reliability of the microelectrode measurements [47].…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic free energy transduction related to the electric potential changes across the thylakoid membrane

1986

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A model based on our present knowledge of photosynthetic energy transduction is presented. Calculated electric potential profiles are compared with microelectrode recordings of the thylakoid electric potential during and after actinic illumination periods of intermediate duration. The information content of the measured electric response is disclosed by a comparison of experimental results with calculations. The proton flux through the ATP synthase complex is seen to markedly influence the electric response. Also the imbalance in maximum turnover rate between the two photosystems, common to obligate shade plants like Peperomia metallica used in the microelectrode experiments, is clearly reflected in the electric potential profile.

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Effect of dicyclohexylcarbodiimide on the proton conductance of thylakoid membranes in intact chloroplast

Cited by 19 publications

References 14 publications

Efficiency of light-driven metabolite transport in the photosynthetic bacterium Rhodospirillum rubrum

Efficiency of light-driven metabolite transport in the photosynthetic bacterium Rhodospirillum rubrum

Suppression of flash-induced PSII-dependent electrogenesis caused by proton pumping in chloroplasts

Photosynthetic free energy transduction related to the electric potential changes across the thylakoid membrane

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