Effect of Dibutyryl Cyclic Adenosine 3',5'-Monophosphate on Morphological Features and Biological Markers of Human Salivary Myoepithelial Cell Line in Culture

Abstract

We have found the emergence of myoepithelial cells (HSG-AZA1) in neoplastic human salivary intercalated duct cell line HSG in culture after treatment with 5-azacytidine. When HSG-ZAZ1 cells were cultured in the presence of N6,O2-dibutyryl cyclic adenosine 3',5'-monophosphate (dB-cAMP), they formed long cytoplasmic processes which were densely packed with ample microfibrils in addition to microtubule bundles. The expression of neuron-specific enolase, synaptophysin, and catecholamine as well as neurofilaments in the treated HSG-AZA1 cells was found by the immunofluorescence staining technique, immunoblotting, immunoelectron microscopy, or catecholamine fluorescence. Both the anchorage-independent and anchorage-dependent growths of HSG-AZA1 cells were suppressed in the presence of dB-cAMP. After the removal of dB-cAMP from the culture, the treated cells returned rapidly to the phenotype and growth rate of the untreated cells. These findings indicate that reversible differentiation into the neuron-like cells of HSG-AZA1 cells occurs in growth medium containing dB-cAMP.

Cell-Based Screen for Identification of Inhibitors of Tubulin Polymerization

“…9 C6 cells quickly change their morphology when db-cAMP is added, as previously reported for other glioma and blastoma cells. [10][11][12] Prior to introduction of db-cAMP, they exhibit glial (fibroblastic) morphology characterized by elongated stoma with two or more processes. After db-cAMP addition, they become spherical with thin neurites.…”

Expression and cellular localization of TSC-22 in normal salivary glands and salivary gland tumors: Implications for tumor cell differentiation

“…It is well known that salivary gland tumors consist of several tumor cell components, such as myoepithelial-like tumor cells, duct-like tumor cells, acinus-like tumor cells, epidermoid-like tumor cells, mucin-producing tumor cells and tumor cells with mesenchymal differentiation (17). There are several reports concerning the direction of differentiation in salivary gland tumors or the tumor stem cells of salivary gland tumors (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33). Sato et al established a cultured salivary gland tumor cell line, HSG (21) and investigated the differentiation potentials of HSG cells (22)(23)(24)(25)(26).…”

“…There are several reports concerning the direction of differentiation in salivary gland tumors or the tumor stem cells of salivary gland tumors (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33). Sato et al established a cultured salivary gland tumor cell line, HSG (21) and investigated the differentiation potentials of HSG cells (22)(23)(24)(25)(26). They reported that HSG cells maintained a phenotype with salivary intercalated duct cells, and could differentiate into several cell types, such as myoepithelial-like cells (22), acinus-like cells (22), squamous cell-like cells (23), chondrocyte-like cells (24), neuronal cell-like cells (25) and osteoblast-like cells (26).…”

“…Sato et al established a cultured salivary gland tumor cell line, HSG (21) and investigated the differentiation potentials of HSG cells (22)(23)(24)(25)(26). They reported that HSG cells maintained a phenotype with salivary intercalated duct cells, and could differentiate into several cell types, such as myoepithelial-like cells (22), acinus-like cells (22), squamous cell-like cells (23), chondrocyte-like cells (24), neuronal cell-like cells (25) and osteoblast-like cells (26). It was concluded that the HSG cells or transformed intercalated duct cells might be the tumor stem cells for several components of the salivary gland tumors.…”

“…It was concluded that the HSG cells or transformed intercalated duct cells might be the tumor stem cells for several components of the salivary gland tumors. On the other hand, under the basis of the observation of histopathology of salivary gland tumors, Aigner et al (27) and several other pathologists (28)(29)(30)(31)(32)(33) reported that myoepithelial-like tumor cells might be the tumor stem cells or procurer cells for several components in salivary gland tumors (22)(23)(24)(25)(26)(27)(28).…”