Effect of Dianhydrodulcitol (DAD) on the Primary Immune Response of Normal and Tumor Bearing Rats

Abstract

The effect of dianhydrodulcithol (DAD) administered in a dose of 5 mg/kg on the number of plaque-forming cells (PFC) in spleens and on the titre of hemolytic antibodies in the sera of rats immunised with sheep red blood cells (SRBC) have been examined. The compound elicited a short-lasting suppressive effect when applied after immunisation; the number of PFC decreased two days after treatment while 5 days after drug administration increased as compared to the untreated animals. Treatment prior to immunisation proved to be ineffective. The titre of circulating antibodies showed changes similar although of lesser extent. Five days following treatment marked infiltration of plasmacytes was present in the axillary lymph nodes. Rats bearing transplantable Guerin tumor showed similar changes in immune response as the tumor free animals following DAD treatment.

Changes in Immune Response to Sheep Red Blood Cells during Growth of Transplantable Rat Tumor

“…The circulating hemolytic antibodies in the individual sera were titrated in Takatsy's microtitrator [2], as de scribed earlier [3], The highest dilutions producing complete hemo lysis and the latest dilutions producing no effect were registered. The titer of 7 S (IgG-typc, ME-resistant) antibodies was determined after digestion of the sera with 0,2 M 2-mercaptoethanol for 30 min at 37 °C [4).…”

Inhibitory and Stimulatory Effects of Dianhydrogalactitol, an Alkylating Cytostatic Agent on the Humoral Immune Response

“…The circulating hemolytic and hemagglulinating antibodies of individual sera were titrated in Tak&tsy's microtitrator [16] as de scribed earlier [5], The lowest dilutions producing no lysis or ag glutination were recorded. The t tcr of 7-S (IgG-type.…”

“…The drug proved to be effective and well tolerated also in humans. DAG had a suppressive effect on the immune system inhibiting the in vitro cytostatic activity of lym phocytes [14] and the humoral immune responsiveness of rats [5], Alterations in the immunomodulating ef fect depending on dose or schedule of treatment were described with numerous cytostatic agents [3,8,10]. This work was done to clarify to what extent the dose and time of DAG treatment would influence the hu moral immune response to sheep red blood cell (SRBC) antigen.…”