Effect of Dental Pulp Stem Cells (DPSCs) in Repairing Rabbit Alveolar Bone Defect

Muhetaer, Huojia; Wu, Zhongming; Zhang, Xiaoli; Maimaitiyiming, Maibubaimu; Ma, Rong

doi:10.7754/clin.lab.2015.150326

Cited by 10 publications

(8 citation statements)

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“…The cells in each well were incubated with 0.2 ml mouse anti-human Vimentin (1: 5,000, ab92547; Abcam, Cambridge, UK) at 4℃ overnight and rinsed under PBS three times after removal of the primary antibody. Subsequently, cells in each well were incubated with 0.2 ml secondary antibody at 37℃ for 30 min and observed under a fluorescence microscope (Huojia, Wu, Zhang, Maimaitiyiming, & Rong, 2015;Liu et al, 2018).…”

Section: Immunofluorescence Assaymentioning

confidence: 99%

Retracted: Downregulated microRNA‐488 enhances odontoblast differentiation of human dental pulp stem cells via activation of the p38 MAPK signaling pathway

Zhao

Cheng

et al. 2018

Journal Cellular Physiology

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Human dental pulp stem cells (hDPSCs) are primarily derived from the pulp tissues of permanent third molar teeth. They were widely used in human bone tissue engineering. It was previously indicated that microRNA (miR) expressions are closely associated with hDPSCs development. However, the specific effect of miR-488 on hDPSCs still remains unclear. In this study, we aimed to investigate effects of miR-488 on the differentiation of hDPSCs into odontoblast cells through the p38 mitogen-activated protein kinases (MAPK) signaling pathway by binding to MAPK1. The hDPSCs were isolated and cultured in vitro. Dual-luciferase reporter gene assay was performed to test the relationship between MAPK1 (p38) and miR-488. Reverse transcription quantitative polymerase chain reaction and western blot analysis were used to detect the mRNA and protein expressions of p38 MAPK signaling pathway-related genes (MAPK1, Ras, and Mitogen-activated protein kinase kinase 3/6 [MKK3/6]), along with expressions of dentin Sialophosphoprotein (DSPP), alkaline phosphatase (ALP), and osteonectin (OCN). ALP staining and alizarin red staining were conducted to detect ALP activity and degree of mineralization. Initially, we found that MAPK1 was the target gene of miR-488. Besides, downregulation of miR-488 was observed to stimulate the p38 MAPK signaling pathway and to increase the messenger RNA and protein expressions of DSPP, ALP, and OCN. Furthermore, ALP activity and formation of a mineralized nodule in hDPSCs were enhanced upon downregulation of miR-488. The aforementioned findings provided evidence supporting that downregulation of miR-488 promotes odontoblastic differentiation of hDPSCs through the p38 MAPK signaling pathway by targeting MAPK1, paving the basis for further study about hDPSCs.

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Section: Immunofluorescence Assaymentioning

confidence: 99%

Retracted: Downregulated microRNA‐488 enhances odontoblast differentiation of human dental pulp stem cells via activation of the p38 MAPK signaling pathway

Zhao

Cheng

et al. 2018

Journal Cellular Physiology

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“…DPSCs show MSC characteristics and can differentiate into many cell types, including odontoblasts, osteoblasts, and chondrocytes. DPSCs are, therefore, capable of differentiation into bone cells, promoting both the regeneration and repair of alveolar bone defects (Huojia et al, ). de Mendonca Costa et al () have transplanted human DPSC into large rat calvarial defects and found that they can differentiate into osteogenic cells without rejection of the graft.…”

Section: Introductionmentioning

confidence: 99%

Stem cells applications in bone and tooth repair and regeneration: New insights, tools, and hopes

Meguid

et al. 2017

Journal Cellular Physiology

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The exploration of stem and progenitor cells holds promise for advancing our understanding of the biology of tissue repair and regeneration mechanisms after injury. This will also help in the future use of stem cell therapy for the development of regenerative medicine approaches for the treatment of different tissue-species defects or disorders such as bone, cartilages, and tooth defects or disorders. Bone is a specialized connective tissue, with mineralized extracellular components that provide bones with both strength and rigidity, and thus enable bones to function in body mechanical supports and necessary locomotion process. New insights have been added to the use of different types of stem cells in bone and tooth defects over the last few years. In this concise review, we briefly describe bone structure as well as summarize recent research progress and accumulated information regarding the osteogenic differentiation of stem cells, as well as stem cell contributions to bone repair/regeneration, bone defects or disorders, and both restoration and regeneration of bones and cartilages. We also discuss advances in the osteogenic differentiation and bone regeneration of dental and periodontal stem cells as well as in stem cell contributions to dentine regeneration and tooth engineering.

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“…These DPSCs generated new bone via direct differentiation into an osteogenic lineage and stimulation of angiogenesis from the host tissue [ 100 ]. Rabbit autologous DPSCs expressed vimentin and CD44 when used (1 × 10 8 ) with Bio-Oss ® scaffolding in the alveolar bone defects of rabbit toothless jaws, regenerating bone with abundant osteoblasts compared to the few osteoblasts that were seen in the scaffold-only group [ 101 ]. Zhang W et al, combined human DPSCs with tyrosine-derived polycarbonate polymer scaffolds E1001(1K) containing beta-tricalcium phosphate (β-TCP) [E1001(1K)/β-TCP] and compared this group to BMP2 (4 µg) in a rat mandibular ramus critical-size bone defect repair model.…”

Section: Dental Pulp Stem Cells and Periodontal Ligament Stem Cellsmentioning

confidence: 99%

Stem Cells and Bone Tissue Engineering

Gao,

Ruzbarsky,

Layne

et al. 2024

Life

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Segmental bone defects that are caused by trauma, infection, tumor resection, or osteoporotic fractures present significant surgical treatment challenges. Host bone autograft is considered the gold standard for restoring function but comes with the cost of harvest site comorbidity. Allograft bone is a secondary option but has its own limitations in the incorporation with the host bone as well as its cost. Therefore, developing new bone tissue engineering strategies to treat bone defects is critically needed. In the past three decades, the use of stem cells that are delivered with different scaffolds or growth factors for bone tissue engineering has made tremendous progress. Many varieties of stem cells have been isolated from different tissues for use in bone tissue engineering. This review summarizes the progress in using different postnatal stem cells, including bone marrow mesenchymal stem cells, muscle-derived stem cells, adipose-derived stem cells, dental pulp stem cells/periodontal ligament stem cells, periosteum stem cells, umbilical cord-derived stem cells, peripheral blood stem cells, urine-derived stem cells, stem cells from apical papilla, and induced pluripotent stem cells, for bone tissue engineering and repair. This review also summarizes the progress using exosomes or extracellular vesicles that are delivered with various scaffolds for bone repair. The advantages and disadvantages of each type of stem cell are also discussed and explained in detail. It is hoped that in the future, these preclinical results will translate into new regenerative therapies for bone defect repair.

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Effect of Dental Pulp Stem Cells (DPSCs) in Repairing Rabbit Alveolar Bone Defect

Cited by 10 publications

References 0 publications

Retracted: Downregulated microRNA‐488 enhances odontoblast differentiation of human dental pulp stem cells via activation of the p38 MAPK signaling pathway

Retracted: Downregulated microRNA‐488 enhances odontoblast differentiation of human dental pulp stem cells via activation of the p38 MAPK signaling pathway

Stem cells applications in bone and tooth repair and regeneration: New insights, tools, and hopes

Stem Cells and Bone Tissue Engineering

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