Effect of Dark-repair Inhibitors on the Survival of<i>Escherichia Coli</i>B under Different Post-irradiation Conditions

Vízdalová, M.; Janovská, E; Zhestjanikov, V. D.

doi:10.1080/09553007114550861

Cited by 3 publications

(4 citation statements)

References 18 publications

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“…Caffeine has been reported to bring about an immediate although reversible inhibition of both the synthesis of ribonucleic acid (RNA) and of protein in sensitive bacteria (37) and its action as a potent mutagen by inhibition of repair processes as well as several recent studies attest (21,43). Methylxanthines are also known to inhibit a variety of eucaryotic cyclic adenosine monophosphate phosphodiesterases (9,15,33) and this may be the primary event responsible for many of the physiological effects of the drug with higher animals.…”

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confidence: 99%

Metabolism of N-methylpurines by a Pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen

Woolfolk¹

1975

J Bacteriol

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Pseudomonas putida, strain 40, originally isolated by enrichment on caffeine as the sole source of carbon and nitrogen, has been developed to grow on 0.5% caffeine. The organism will grow on any N-methyl derivative of xanthine containing one or more methyl groups at the 1, 3, or 7 positions. An investigation of the activities of resting cell suspensions and cell-free preparations together with the detection of metabolic intermediates suggest that caffeine is first metabolized by the action of an enzyme which is capable of hydrolytically removing all three methyl groups with the production of methanol and free xanthine. The methanol presumably is oxidized to the final product, CO2, through the sequential action of methanol, formaldehyde, and formate dehydrogenases, which are induced by growth on caffeine. Furthermore, the xanthine would seem to be channeled through conventional pathways of purine degradation through the action of xanthine dehydrogenase and uricase, both induced by growth on caffeine. However, a variety of data suggests that the metabolism of caffeine may be compartmentalized in the cell and metabolized separately from externally added xanthine. Additional studies indicated that the cell is permeable to the methylxanthines. The significance of these findings'is discussed.

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Metabolism of N-methylpurines by a Pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen

Woolfolk¹

1975

J Bacteriol

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“…provoquees par le rayonnement U .V. (Witkin 1958, Sideropoulos et Shankel 1968, Mouton et Tremeau 1969, Vizdalova, Jonovska et Zhestjanikov 1971 1969) influence des conditions de croissance (Apelgot 1968)) . Il nous a done paru interessant d'etudier, dans nos experiences, le role de la cafeine, qui, dans le cas de bacteries a radioresistance elevee, reste sans effet sur la survie aux rayons y ou x, mais diminue leur resistance aux rayons u .v .…”

Section: Resultatsunclassified

“…Il nous a done paru interessant d'etudier, dans nos experiences, le role de la cafeine, qui, dans le cas de bacteries a radioresistance elevee, reste sans effet sur la survie aux rayons y ou x, mais diminue leur resistance aux rayons u .v . (Mouton et Tremeau 1969et Vizdalova et al 1971 . Nous esperions pouvoir, grace a ce compose, dissocier differents mecanismes de reparation.…”

Section: Resultatsunclassified

“…On doit noter que, dans ces conditions, la cafeine est sans action sur la survie des bacteries (figures 5 et 6), comme elle est sans action dans les irradiations y ou x, alors qu'elle augmente la sensibilite des memes bacteries irradiees par les u .v . (Mouton et Tremeau 1969et Vizdalova et al 1971 . Ces resultats montrent que les lesions letales du type B .…”

Section: Role De L'activite Specifiqueunclassified

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Variation, en Fonction des Conditions Expérimentales, de l'Efficacité des Désintégrations de³²P Incorporé ChezE. Coli

Apelgot

1972

International Journal of Radiation Biology and Related Studies

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Les experiences decrites montrent que, pour les bacteries de la souche utilisee, la valeur de a (probabilite d'inactivation de chaque transmutation de 32P incorpore) depend de 1'activite specifique utilisee . A chacune des deux temperatures etudiees (0 et -196°c) it existe deux valeurs de a . La plus grande, as, caracterise les activites specifiques inferieures, et la plus basse, a r , les activites specifiques plus elevees . La concentration correspondant au passage de a g a a r depend de la temperature d'experience et se situe entre 8 et 15 Ci/g P . En ajoutant la D-L-pantoyl-lactone au milieu de croissance, nous avons pu demontrer que ce resultat West pas en relation avec la formation de formes`serpents 'qui apparaissent lorsque la croisssance bacterienne se fait en presence de $ 2 P a activite specifique elevee. En ajoutant la cafeine dans le milieu de post-traitement, au moment du denombrement des bacteries, nous avons pu mettre en evidence l'action de ce compose. Ces resultats demontrent que les bacteries peuvent reparer certaines des lesions produites par les transmutations des atomes de 32P incorpores .

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Degradation of purines and pyrimidines by microorganisms

Vogels¹,

Drift²

1976

Bacteriol Rev

332

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Effect of Dark-repair Inhibitors on the Survival ofEscherichia ColiB under Different Post-irradiation Conditions

Cited by 3 publications

References 18 publications

Metabolism of N-methylpurines by a Pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen

Metabolism of N-methylpurines by a Pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen

Variation, en Fonction des Conditions Expérimentales, de l'Efficacité des Désintégrations de³²P Incorporé ChezE. Coli

Degradation of purines and pyrimidines by microorganisms

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Effect of Dark-repair Inhibitors on the Survival ofEscherichia ColiB under Different Post-irradiation Conditions

Cited by 3 publications

References 18 publications

Metabolism of N-methylpurines by a Pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen

Metabolism of N-methylpurines by a Pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen

Variation, en Fonction des Conditions Expérimentales, de l'Efficacité des Désintégrations de32P Incorporé ChezE. Coli

Degradation of purines and pyrimidines by microorganisms

Contact Info

Product

Resources

About

Variation, en Fonction des Conditions Expérimentales, de l'Efficacité des Désintégrations de³²P Incorporé ChezE. Coli