Effect of D-003, a Mixture of Very High Molecular Weight Aliphatic Acids, on Prednisolone-Induced Osteoporosis in Sprague-Dawley Rats

Noa, Miríam; Mendoza, Sarahí; Más, Rosa; Mendoza, Nilda; León, Felipe Rodríguez De

doi:10.2165/00126839-200405050-00004

Cited by 17 publications

(9 citation statements)

References 45 publications

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“…GC administration has been shown to induce loss of the cancellous BV/TV, Tb N, and Tb Th of the lumbar spine in rats and also loss of the three-dimensional cancellous BV/TV and Tb Th, an increase in the trabecular bone pattern factor in the lumbar spine of minipigs (15)(16)(17)(18)(19)(20)(21). GC-induced cancellous osteopenia has been shown to be associated with decreased bone formation and increased bone resorption, and the key histological Fig.…”

Section: Discussionmentioning

confidence: 99%

Effects of Alfacalcidol on Cancellous and Cortical Bone Mass in Rats Treated with Glucocorticoid: A Bone Histomorphometry Study

Iwamoto

Seki

Takeda

et al. 2007

J Nutr Sci Vitaminol

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RetractionThe following articles have been retracted by the Editorial Board of Journal of Nutritional Science and Vitaminology, because some parts of their contents were published in other journals. , 191-197, 2007 J Nutr Sci Vitaminol , 53 , 191-197, 2007 191 Summary The beneficial effects of alfacalcidol (ALF) on bone mass, bone formation, and bone resorption have been established in ovariectomized rats. Our previous studies showed that high-dose glucocorticoid (GC) administration (methylprednisolone sodium succinate, 5.0 mg/kg, s.c., 3 times a week) for 4 wk induced cancellous osteopenia without significantly affecting cortical bone mass in Sprague-Dawley rats, and that high-dose GC administration for 8 wk also resulted in cortical osteopenia. The purpose of the present study was to examine the effects of ALF on cancellous and cortical bone mass in GC-treated rats. Forty female Sprague-Dawley rats, 3 mo of age, were randomized by the stratified weight method into four groups of 10 rats each, as follows: age-matched control group (CON); 8-wk GC administration with administration of vehicle during the latter 4 wk of treatment (GC group); 8-wk GC administration with administration of a low dose of ALF (0.08 g/kg) during the latter 4 wk of treatment (low-dose ALF group); 8-wk administration of GC with administration of a high dose of ALF (0.16 g/kg) during the latter 4 wk of treatment (highdose ALF group). The GC (methylprednisolone sodium succinate, 5.0 mg/kg) was administered subcutaneously 3 times a week, and ALF was administered orally 5 times a week. At the end of the experiment, static and dynamic bone histomorphometric analyses were performed on cancellous bone of the proximal tibial metaphysis and cortical bone of the tibial diaphysis. Eight-week GC administration resulted in loss of the cancellous bone volume/total tissue volume (BV/TV) and percent cortical area (Ct Ar) as a result of decreased trabecular bone formation, increased trabecular and endocortical bone resorption, and decreased periosteal bone formation. Low-dose ALF restored the cancellous BV/TV by mildly suppressing bone resorption and restoring bone formation, whereas high-dose ALF increased it beyond the value observed in the age-matched controls by strongly suppressing bone resorption and markedly increasing bone formation. Both low-and high-dose ALF prevented the GCinduced reduction of the percent Ct Ar by increasing periosteal bone formation and suppressing endocortical bone resorption. The effects of ALF on cancellous bone mass, bone formation, and bone resorption were all dose-dependent. The present study showed the beneficial effects of ALF on cancellous and cortical bone mass in GC-treated rats. Effects of Alfacalcidol on Cancellous and Cortical Bone Mass in Rats Treated with Glucocorticoid: A Bone Histomorphometry Study

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Section: Discussionmentioning

confidence: 99%

Effects of Alfacalcidol on Cancellous and Cortical Bone Mass in Rats Treated with Glucocorticoid: A Bone Histomorphometry Study

Iwamoto

Seki

Takeda

et al. 2007

J Nutr Sci Vitaminol

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“…It has been demonstrated that GC induces the loss of cancellous BV/TV, Tb N, and Tb Th, decreases longitudinal bone growth and femoral bone length, and reduces the mechanical strength of the lumbar spine [5,6,[15][16][17]22]. GC-induced osteopenia has been associated with decreased bone formation, with the effects on bone resorption in terms of osteoclastic activity being inconsistent.…”

Section: Discussionmentioning

confidence: 99%

Preventive Effects of Risedronate and Calcitriol on Cancellous Osteopenia in Rats Treated with High-Dose Glucocorticoid

et al. 2006

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“…GC administration has been shown to induce a loss in 2D cancellous BV/TV, Tb N, and Tb Th, coupled with deterioration of the mechanical properties of the lumbar spine in rats (17)(18)(19)(20)(21)(22); It has also been shown to induce a loss in 3D cancellous BV/TV and Tb Th, with an increase in trabecular bone pattern factor and deterioration of the mechanical properties of the lumbar spine in minipigs ( 23 ). However, long-term administration of GCs may be needed before 2D and 3D cancellous bone structures are modified in animals ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Effects of Risedronate and Calcitriol on Cancellous Bone in Rats with Glucocorticoid-Induced Osteopenia

Iwamoto

Seki

Takeda

et al. 2006

J Nutr Sci Vitaminol

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Summary Objectives: To compared the effects of risedronate (Ris) and calcitriol (Cal) on cancellous bone in rats with glucocorticoid (GC)-induced osteopenia. Materials and Methods: Thirty female Sprague-Dawley rats, 4 mo of age, were randomly divided by the stratified weight method into three groups of 10 rats each according to the following treatment schedule: 8-wk GC administration with 4-wk vehicle (control), Ris, and Cal as therapeutic treatment initiated after 4-wk GC administration. The GC (methylprednisolone sodium succinate, 5.0 mg/kg, s.c.), Ris (10 g/kg, s.c.), and Cal (0.1 g/kg, p.o.) were administered 3 times a week. At the end of the 8-wk treatment period, two-dimensional (2D) bone histomorphometric analysis was performed for cancellous bone of the proximal tibial metaphysis, 3D micro-computed tomographic analysis was performed for the distal femoral metaphysis, and the mechanical strength of the distal femoral metaphysis was evaluated by a compression test. Results: Ris and Cal treatment increased both 2D and 3D cancellous bone mass. However, Ris treatment exhibited more pronounced effects on 2D and 3D cancellous bone mass than Cal treatment, and the effects of both Ris and Cal treatment were greater on 3D cancellous bone mass than on 2D cancellous bone mass. The response of 2D and 3D cancellous bone mass to Ris treatment was characterized by its effect on trabecular number and thickness, which was associated with markedly suppressed bone resorption and bone formation in terms of suppressed bone turnover. On the other hand, the response of 2D cancellous bone mass to Cal treatment was attributed to the effect of Cal on 2D trabecular thickness, and the response of 3D cancellous bone mass to Cal treatment might be characterized by the effect of Cal on 3D trabecular number and thickness, with a more marked effect of trabecular thickness. These effects were primarily due to mildly suppressed bone resorption and maintained or even increased bone formation. Despite the differential effect of Ris and Cal treatment on the cancellous bone structure and bone metabolism, both treatment increased the maximum load and braking energy of the distal femoral metaphysis to a similar extent, suggesting different mechanisms for improving bone strength. Conclusions: This study showed the differential effects of Ris and Cal on cancellous bone in rats with GC-induced osteopenia.

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Effect of D-003, a Mixture of Very High Molecular Weight Aliphatic Acids, on Prednisolone-Induced Osteoporosis in Sprague-Dawley Rats

Cited by 17 publications

References 45 publications

Effects of Alfacalcidol on Cancellous and Cortical Bone Mass in Rats Treated with Glucocorticoid: A Bone Histomorphometry Study

Effects of Alfacalcidol on Cancellous and Cortical Bone Mass in Rats Treated with Glucocorticoid: A Bone Histomorphometry Study

Preventive Effects of Risedronate and Calcitriol on Cancellous Osteopenia in Rats Treated with High-Dose Glucocorticoid

Comparative Effects of Risedronate and Calcitriol on Cancellous Bone in Rats with Glucocorticoid-Induced Osteopenia

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