Effect of cytokine-induced killer cells on immune function in patients with lung cancer

Abstract

Abstract. Cytokine-induced killer (CIK) cells have been used as adoptive immunotherapy in cancer. The present study evaluated the effect of CIK cells on immune function in patients with lung cancer. Patients were divided into three groups, according to the treatment received prior to CIK cell treatment: CIK group (no prior treatment), Che-Sur group (prior chemotherapy and surgery) and Che-Rad group (prior chemotherapy and radiotherapy). Following treatment, the average percentage of cluster of differentiation (CD)3 + CD4 + , CD3 + , natural killer (NK) and NKT cells in peripheral blood was significantly higher than that prior to CIK treatment in the Che-Sur and CIK groups, and the levels of interferon-γ in serum were significantly higher than those prior to CIK treatment in the Che-Sur and CIK groups. On the contrary, the levels of interleukin-10 had decreased in these groups following CIK treatment. Subsequently, patients were divided into three groups according to the percentage of CD3 + CD56 + CIK cells that were administered to the patients. The number of NK and NKT cells increased with increasing number of CD3 + CD56 + cells. The patients in the CIK and Che-Sur groups were the most benefited ones following CIK treatment, contrarily to those in the Che-Rad group, since the increase in the number of CD3 + CD56 + CIK cells in the aforementioned patients enhanced the number of NK cells, which exhibit antitumor activity.

The Immune Regulatory Role of Cytokine-Induced Killer Cells Treatment on Non-Small Cell Lung Cancer Patients

“…Many studies including our study found that the average percentage of CD3 + CD4 + , CD3 + and NK T-cells, as well as the levels of IFN-γ following several treatment courses, were significantly higher than the values observed prior to CIK cells treatment [50][51][52]. Of course, there are studies found that there are no changes after CIK cells treatment [25].…”

“…Otherwise, the treatment done before CIK cells treatment may affect the immune status of NSCLS patients [50]. Thus, the treatments administered to the patients prior to CIK cells therapy seemed to affect the outcome, since more courses were required to achieve effective antitumor immune responses.…”

Cytokine‑induced killer cells/natural killer cells combined with anti‑GD2 monoclonal antibody increase cell death rate in neuroblastoma SK‑N‑SH cells

“…CIK cells are mononuclear cells that can be isolated from peripheral blood or cord blood. The cells are cultured with various cytokines and are CD3 + CD56 + (48). Due to the expression of CD3 and CD56 on the cell surface, CIK cells not only demonstrate anti-tumor activity, but also have non-major histocompatibility complex-restricted killing activity (49).…”

Interleukin gene polymorphisms in Chinese Han population with breast cancer, a case-control study

“…The role of cytokines in cancer immunization and carcinogenesis has been recognized [ 7 ]. Many studies of cytokine polymorphisms associated with susceptibility to [ 8 , 9 ], liver cancer [ 10 , 11 ], lung cancer [ 12 ], prostate cancer [ 13 ] and ovarian cancer [ 14 ] are mixed.…”