Effect of cytochalasin A on actin distribution in the fission yeastSchizosaccharomyces pombe studied by fluorescent and electron microscopy

Kanbe, Toshio; Akashi, Tomohiro; Tanaka, Kenji

doi:10.1007/bf01378936

Cited by 21 publications

(16 citation statements)

References 17 publications

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“…12A; Osumi et al, 1989a;Kobori et al, 1989) and anti-actin antibody-conjugated colloidal gold particles in immunoelectron microscopy (manuscript in preparation). Although little is known about filasomes, the TEM and FLM findings suggest that they are closely associated with F-actin (Kanbe et al, 1993(Kanbe et al, , 1994.…”

Section: Dynamics Of Intracellular Organelles Observed By Temmentioning

confidence: 95%

Dynamics of Cell Wall Formation in Fission Yeast,Schizosaccharomyces pombe

Osumi

Sato

Ishijima

et al. 1998

Fungal Genetics and Biology

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Section: Dynamics Of Intracellular Organelles Observed By Temmentioning

confidence: 95%

Dynamics of Cell Wall Formation in Fission Yeast,Schizosaccharomyces pombe

Osumi

Sato

Ishijima

et al. 1998

Fungal Genetics and Biology

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“…The effect of cytochalasin D. Cytochalasin D disrupts the actin microfilament networks in fungal hyphae (Kanbe et al, 1993), including those in the fruiting bodies of Flammulina velutipes, in which it causes a loss of 80 % of stem gravitropic bending capability (Monzer, 1995). The lowest concentration of cytochalasin D that caused noticeable changes in the gravitropic reaction of C. cinereus stems was 1 µM (Fig.…”

Section: Fitting the Model To The Gravitropic Reactions Of Inhibitor-mentioning

confidence: 99%

Mathematical modelling of morphogenesis in fungi: a key role for curvature compensation (‘autotropism’) in the local curvature distribution model

1999

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The assumption that the mushroom stem has the ability to undergo autonomic straightening enables a mathematical model to be written that accurately mimics the gravitropic reaction of the stems of Coprinus cinereus. The straightening mechanism is called curvature compensation here, but is equivalent to the ' autotropism ' that often accompanies the gravitropic reactions of axial organs in plants. In the consequently revised local curvature distribution model, local bending rate is determined by the difference between the ' bending signal ' (generated by gravitropic signal perception systems) and the ' straightening signal ' (proportional to the local curvature at the given point). The model describes gravitropic stem bending in the standard assay with great accuracy but has the virtue of operating well outside the experimental data set used in its derivation. It is shown, for example, that the mathematical model can be fitted to the gravitropic reactions of stems treated with metabolic inhibitors by a change of parameters that parallel the independently derived physiological interpretation of inhibitor action. The revised local curvature distribution model promises to be a predictive tool in the further analysis of gravitropism in mushrooms.

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“…F-actin cables and microtubules are oriented along the long axis of the cell independently from each other (Kanbe et al, 1989;Arai et al, 1998). F-actin is essential for polarized growth and cytokinesis and may serve as tracks for the polarized transport of secretory vesicles to the growth site (Kanbe et al, 1989(Kanbe et al, , 1993. Unlike the situation in budding yeast (Huffaker et al, 1988;Jacobs et al, 1998), microtubules are involved in maintaining the direction of polarized cell growth, although they are not involved in the growth itself Umesono et al, 1983;Hiraoka et al, 1984;Radcliffe et al, 1998;Sawin and Nurse, 1998).…”

Section: Introductionmentioning

confidence: 99%

Identification of Two Type V Myosins in Fission Yeast, One of Which Functions in Polarized Cell Growth and Moves Rapidly in the Cell

Motegi

Arai

Mabuchi

2001

MBoC

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We characterized the novel Schizosaccharomyces pombe genes myo4 ϩ and myo5 ϩ , both of which encode myosin-V heavy chains. Disruption of myo4 caused a defect in cell growth and led to an abnormal accumulation of secretory vesicles throughout the cytoplasm. The mutant cells were rounder than normal, although the sites for cell polarization were still established. Elongation of the cell ends and completion of septation required more time than in wild-type cells, indicating that Myo4 functions in polarized growth both at the cell ends and during septation. Consistent with this conclusion, Myo4 was localized around the growing cell ends, the medial F-actin ring, and the septum as a cluster of dot structures. In living cells, the dots of green fluorescent protein-tagged Myo4 moved rapidly around these regions. The localization and movement of Myo4 were dependent on both F-actin cables and its motor activity but seemed to be independent of microtubules. Moreover, the motor activity of Myo4 was essential for its function. These results suggest that Myo4 is involved in polarized cell growth by moving with a secretory vesicle along the F-actin cables around the sites for polarization. In contrast, the phenotype of myo5 null cells was indistinguishable from that of wild-type cells. This and other data suggest that Myo5 has a role distinct from that of Myo4.

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Effect of cytochalasin A on actin distribution in the fission yeastSchizosaccharomyces pombe studied by fluorescent and electron microscopy

Cited by 21 publications

References 17 publications

Dynamics of Cell Wall Formation in Fission Yeast,Schizosaccharomyces pombe

Dynamics of Cell Wall Formation in Fission Yeast,Schizosaccharomyces pombe

Mathematical modelling of morphogenesis in fungi: a key role for curvature compensation (‘autotropism’) in the local curvature distribution model

Identification of Two Type V Myosins in Fission Yeast, One of Which Functions in Polarized Cell Growth and Moves Rapidly in the Cell

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