Dynamics of Cell Wall Formation in Fission Yeast,Schizosaccharomyces pombe

Osumi, Masako; Sato, Mamiko; Ishijima, Sanae A.; Konomi, Mami; Такаги, Таичиро; Yaguchi, Hiroko

doi:10.1006/fgbi.1998.1067

Cited by 62 publications

(55 citation statements)

References 63 publications

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“…Thus it would be of interest to investigate the manner of tER distribution in other instances where Golgi accumulation at the growth sites was reported (Rida et al, 2006). Interestingly, the Golgi and the secretory vesicles accumulated at the sites of cell wall formation as judged by three-dimensional (3D) reconstruction of reverting S. pombe protoplasts (Osumi et al, 1998). The importance of ER recruitment has been recently reported in a study on the role of the gene jagunal in Drosophila oocyte development (Lee and Cooley, 2007).…”

Section: Polarization Of the Secretory Pathway To The Division Sitementioning

confidence: 94%

The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast

Vještica

Tang

Oliferenko

2008

MBoC

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The ultimate goal of cytokinesis is to establish a membrane barrier between daughter cells. The fission yeast Schizosaccharomyces pombe utilizes an actomyosin-based division ring that is thought to provide physical force for the plasma membrane invagination. Ring constriction occurs concomitantly with the assembly of a division septum that is eventually cleaved. Membrane trafficking events such as targeting of secretory vesicles to the division site require a functional actomyosin ring suggesting that it serves as a spatial landmark. However, the extent of polarization of the secretion apparatus to the division site is presently unknown. We performed a survey of dynamics of several fluorophore-tagged proteins that served as markers for various compartments of the secretory pathway. These included markers for the endoplasmic reticulum, the COPII sites, and the early and late Golgi. The secretion machinery exhibited a marked polarization to the division site. Specifically, we observed an enrichment of the transitional endoplasmic reticulum (tER) accompanied by Golgi cisternae biogenesis. These processes required actomyosin ring assembly and the function of the EFC-domain protein Cdc15p. Cdc15p overexpression was sufficient to induce tER polarization in interphase. Thus, fission yeast polarizes its entire secretory machinery to the cell division site by utilizing molecular cues provided by the actomyosin ring. INTRODUCTIONCell division is the final event in the cell cycle that results in physical separation of two daughter cells. Despite being studied for more than a hundred years, the underlying molecular mechanisms and the cytological details of the process are still emerging. Various organisms and cell types have established multiple pathways to conduct cell division that are regulated at both signaling and structural levels (for review see Balasubramanian et al., 2004). In recent years, the fission yeast Schizosaccharomyces pombe has emerged as an attractive model for the study of cytokinesis because of its fully sequenced genome (Wood et al., 2002), a cell size convenient for cytological studies and the availability of a large set of conditional mutants compromised in various aspects of cell division (Chang et al., 1996;Balasubramanian et al., 1998).On entry into mitosis, S. pombe assembles an actomyosin ring that is thought to drive a binary cell fission through constriction, similar to many other eukaryotes, including nematodes, insects, and vertebrates (for review see Hales et al., 1999). During early mitosis, actin is assembled into a ring structure through the action of several actin nucleating and bundling proteins and molecular motors. These include the formin Cdc12p (Chang et al., 1997), profilin Cdc3p (Balasubramanian et al., 1992), the extended Fer/CIP4 (EFC) domain protein Cdc15p (Fankhauser et al., 1995), and the myosin heavy chain Myo2p (Kitayama et al., 1997) together with its light chains Cdc4p (McCollum et al., 1995;Naqvi et al., 1999) and Rlc1p (Le Goff et al., 2000). It has been proposed tha...

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Section: Polarization Of the Secretory Pathway To The Division Sitementioning

confidence: 94%

The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast

Vještica

Tang

Oliferenko

2008

MBoC

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“…First, it is detected as a tangled network of ␤(1,3)-Dglucan fibrils enveloping the spherical protoplast. Next, the fibrous network evolves and the protoplast cells acquire a more elongated shape, resulting in bundles and ribbons of ␤(1,3)-Dglucan surrounded by galactomannan particles (35,36). Mature ␣(1,3)-D-glucan has not been well detected by immunoelectron microscopy, but functional and structural analysis in the absence of Ags1 indicates that it is localized with the B-BG in the lesselectron-dense region of the cell wall.…”

Section: Cell Wall and Septum In Fission Yeast Cell Wall Composition mentioning

confidence: 99%

The Cell Biology of Fission Yeast Septation

Cortés

Ramos

Osumi

et al. 2016

Microbiol Mol Biol Rev

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SUMMARYIn animal cells, cytokinesis requires the formation of a cleavage furrow that divides the cell into two daughter cells. Furrow formation is achieved by constriction of an actomyosin ring that invaginates the plasma membrane. However, fungal cells contain a rigid extracellular cell wall surrounding the plasma membrane; thus, fungal cytokinesis also requires the formation of a special septum wall structure between the dividing cells. The septum biosynthesis must be strictly coordinated with the deposition of new plasma membrane material and actomyosin ring closure and must occur in such a way that no breach in the cell wall occurs at any time. Because of the high turgor pressure in the fungal cell, even a minor local defect might lead to cell lysis and death. Here we review our knowledge of the septum structure in the fission yeastSchizosaccharomyces pombeand of the recent advances in our understanding of the relationship between septum biosynthesis and actomyosin ring constriction and how the two collaborate to build a cross-walled septum able to support the high turgor pressure of the cell. In addition, we discuss the importance of the septum biosynthesis for the steady ingression of the cleavage furrow.

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“…Cells were then suspended in MM with 0.8 M sorbitol and grown with gentle shaking (80-90 r.p.m.) (Osumi et al, 1998). Cells were typically imaged four hours after spheroplasting.…”

Section: Yeast Media and Spheroplastingmentioning

confidence: 99%

Cylindrical Cellular Geometry Ensures Fidelity of Division Site Placement in Fission Yeast

Mishra

Huang

Srivastava

et al. 2012

Journal of Cell Science

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SummarySuccessful cytokinesis requires proper assembly of the contractile actomyosin ring, its stable positioning on the cell surface and proper constriction. Over the years, many of the key molecular components and regulators of the assembly and positioning of the actomyosin ring have been elucidated. Here we show that cell geometry and mechanics play a crucial role in the stable positioning and uniform constriction of the contractile ring. Contractile rings that assemble in locally spherical regions of cells are unstable and slip towards the poles. By contrast, actomyosin rings that assemble on locally cylindrical portions of the cell under the same conditions do not slip, but uniformly constrict the cell surface. The stability of the rings and the dynamics of ring slippage can be described by a simple mechanical model. Using fluorescence imaging, we verify some of the quantitative predictions of the model. Our study reveals an intimate interplay between geometry and actomyosin dynamics, which are likely to apply in a variety of cellular contexts.

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Dynamics of Cell Wall Formation in Fission Yeast,Schizosaccharomyces pombe

Cited by 62 publications

References 63 publications

The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast

The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast

The Cell Biology of Fission Yeast Septation

Cylindrical Cellular Geometry Ensures Fidelity of Division Site Placement in Fission Yeast

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