Effect of cyclosporine pretreatment on mitochondrial function in vitrified bovine mature oocytes

Zhao, Xiao‐Fan; Du, Weihua; Wang, Dong; Huang, Hao; Liu, Yan; Tong, Qin; Zhu, Huabin

doi:10.1016/j.fertnstert.2011.04.089

Cited by 48 publications

(40 citation statements)

References 5 publications

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“…4B), as shown by our previous time-dependent observation (Hwang et al 2013). Maintenance of ATP contents in bovine IVM oocytes by cyclosporine treatment can improve parthenogenetic development into blastocysts after oocyte vitrification (Zhao et al 2011). No significant changes were also detected between a-tocopherol-treated and untreated postwarming oocytes in CG distribution (Fig.…”

Section: Discussionsupporting

confidence: 80%

High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

et al. 2015

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The objective of this study was to investigate whether developmental competence of vitrified-warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either L-ascorbic acid or a-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2 h before IVF. The exposure to a-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1-36.3% vs 19.2-25.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between a-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in a-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with a-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10 h post-IVF). It was concluded that a-tocopherol treatment of vitrified-warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts.

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Section: Discussionsupporting

confidence: 80%

High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

et al. 2015

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“…When the mitochondrial membrane potential is low, the efficiency of energy supply by oxidative phosphorylation will drop [45,51]. Cryopreservation compromises mitochondrial activity and decreases ATP concentration in mouse, bovine and human oocytes [22,49,52,53]. This present study showed the ATP concentration of vitrified porcine oocytes after 1 h thawing decreased greatly compared with that of fresh oocytes.…”

Section: Discussionmentioning

confidence: 55%

“…Zander-Fox et al [50] showed that mouse oocytes vitrification altered mitochondrial distribution and membrane potential. Zhao et al [52] found that vitrification had a significantly negative impact on the mitochondrial function of bovine oocytes, and cyclosporine (a specific inhibitor of mitochondrial permeability transition (MPT) pretreatment before vitrification could increase the mitochondrial DWm and improve the developmental ability of vitrified oocytes. Results from these reports were similar to those reported here and indicated that vitrification of porcine MII-stage oocytes resulted in serious damages to mitochondrial membrane potential.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, ROS can decrease embryo developmental ability and promote the occurrence of apoptosis in oocytes and early embryo [8]. Several past studies have emphasized the damage caused by the formation of ROS during cryopreservation processes, leading to oxidative stress [12,21,52]. ROS formed during the cryopreservation process can degrade essential molecules to cells, including the polyunsaturated lipids present in the cell membrane (lipid peroxidation), leading to oocyte death [40].…”

Section: Discussionmentioning

confidence: 99%

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Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability

et al. 2015

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“…Moreover, developmental failure in the preimplantation embryo may result from an abnormal distribution of mitochondria in the oolemma [52]. Vitrification has been reported to compromise mitochondrial function and reduce ATP content in human [84] and bovine [85] oocytes, which might contribute to poor oocyte development after cryopreservation [85]. The intracellular distribution of mitochondria is dependent on microtubules [86], which is important for redistribution of ATP and allows increased levels of ATP to be produced in different intracellular areas during periods of high energy requirements [86,87].…”

Section: Mitochondriamentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. cryopreservation, mammals, oocytes, embryos, cryoinjuries Citation:Moussa M, Shu J, Zhang XH, Zeng FY. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci, 2014, 57: 903 -914,

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Effect of cyclosporine pretreatment on mitochondrial function in vitrified bovine mature oocytes

Cited by 48 publications

References 5 publications

High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

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