Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability

Dai, Jianjun; Wu, Chao; Muneri, Caroline; Niu, Ying‐Fang; Zhang, Shushan; Ren, Rui; Zhang, Defu

doi:10.1016/j.cryobiol.2015.08.002

Cited by 96 publications

(65 citation statements)

References 53 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our previous results indicated that bovine oocyte vitrification could induce apoptosis in blastocysts (Chen et al, ). Many other studies also showed that oocyte vitrification could increase apoptosis in blastocysts (Dai et al, ; Zhao et al, ), which was in line with our results. These results imply that the accumulation of DSBs might, at least in part, contribute to the increased apoptotic rate in blastocysts.…”

Section: Discussionsupporting

confidence: 93%

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

Chang

Chen

Zhang

et al. 2019

Molecular Reproduction Devel

View full text Add to dashboard Cite

As an assisted reproduction technology, vitrification has been widely used for oocyte and embryo cryopreservation. Many studies have indicated that vitrification affects ultrastructure, gene expression, and epigenetic status. However, it is still controversial whether oocyte vitrification could induce DNA damage in metaphase II (MII) oocytes and the resulting early embryos. This study determined whether mouse oocytes vitrification induce DNA damage in MII oocytes and the resulting preimplantation embryos, and causes for vitrification‐induced DNA damage. The effects of oocyte vitrification on reactive oxygen species (ROS) levels, γ‐H2AX accumulation, apoptosis, early embryonic development, and the expression of DNA damage‐related genes in early embryos derived by in vitro fertilization were examined. The results indicated that vitrification significantly increased the number of γ‐H2AX foci in zygotes and two‐cell embryos. Trp53bp1 was upregulated in zygotes, two‐cell embryos and four‐cell embryos in the vitrified group, and Brca1 was increased in two‐cell embryos after vitrification. Vitrification also increased the ROS levels in MII oocytes, zygotes, and two‐cell embryos and the apoptotic rate in blastocysts. Resveratrol (3,5,4′‐trihydroxystilbene) treatment decreased the ROS levels and the accumulation of γ‐H2AX foci in zygotes and two‐cell embryos and the apoptotic rate in blastocysts after vitrification. Overall, vitrification‐induced abnormal ROS generation, γ‐H2AX accumulation, an increase in the apoptotic rate and the disruption of early embryonic development. Resveratrol treatment could decrease ROS levels, γ‐H2AX accumulation, and the apoptotic rate and improve early embryonic development. Vitrification‐associated γ‐H2AX accumulation is at least partially due to abnormal ROS generation.

show abstract

Section: Discussionsupporting

confidence: 93%

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

Chang

Chen

Zhang

et al. 2019

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…Although no direct link has been established between osmotic stress and oocyte apoptosis, cell death through apoptosis were also happened after oocyte vitrification495051. Glycine prevents the cells against apoptosis has been reported in rat endothelial cells52, mice neuronal cells53 and chick embryos54.…”

Section: Discussionmentioning

confidence: 99%

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

Cao

Rose

Wang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Ice-free cryopreservation, referred to as vitrification, is receiving increased attention in the human and animal assisted reproduction. However, it introduces the detrimental osmotic stress by adding and removing high contents of cryoprotectants. In this study, we evaluated the effects of normalizing cell volume regulation by adding glycine, an organic osmolyte, during vitrification of mouse germinal vesicle stage oocyte and/or subsequent maturation on its development. The data showed that glycine supplementation in either vitrification/thawing or maturation medium significantly improved the cytoplasmic maturation of MII oocytes manifested by spindle assembly, chromosomal alignment, mitochondrial distribution, euploidy rate, and blastocyst development following fertilization in vitro, compared to the control without glycine treatment. Furthermore, glycine addition during both vitrification/thawing and maturation further enhanced the oocyte quality demonstrated by various markers, including ATP contents and embryo development. Lastly, the effect of anti-apoptosis was also observed when glycine was added during vitrification. Our result suggests that reducing osmotic stress induced by vitrification could improve the development of vitrified mouse oocyte.

show abstract

“…It is well known that cryopreservation negatively impacts on embryo quality with reports of low developmental ability and ATP content in warmed oocyte and preimplantation embryos (Chen et al, ; Dai et al, ; ; Shi et al, 2; Zhang et al, ). Zhao et al () reported that the ATP content in post‐warming oocytes was low in vitrified‐warmed bovine oocytes.…”

Section: Discussionmentioning

confidence: 99%

Effects of resveratrol treatment on mitochondria and subsequent embryonic development of bovine blastocysts cryopreserved by slow freezing

Hayashi

Kansaku

Abe

et al. 2019

Animal Science Journal

View full text Add to dashboard Cite

This study evaluated the effects of cryopreservation by slow freezing on the mitochondrial function, DNA integrity, and developmental ability of bovine embryos and examined whether resveratrol treatment of the frozen‐thawed blastocysts improved embryonic viability. In vitro produced bovine embryos were subjected to slow freezing. After thawing, the ATP content and mitochondrial DNA integrity (mtDNA), determined by real‐time PCR targeting short and long mitochondrial sequences, was found to be lower in frozen‐thawed embryos than in fresh embryos, and mtDNA copy number was significantly reduced during the 24‐hr incubation post warming. Furthermore, immunostaining against double‐strand DNA revealed DNA damage in frozen‐thawed embryos. When frozen‐thawed embryos were incubated in the medium containing 0.5 µM resveratrol, SIRT1 expression, and survival rate of the embryos significantly improved compared with the vehicle‐treated embryos. In addition, cell‐free mtDNA content in medium was higher in case of resveratrol‐treated embryos than of vehicle‐treated embryos. In conclusion, slow freezing affects mitochondrial integrity and function in the blastocysts. In the frozen‐thawed embryos, mitochondria were removed during post‐thawing incubation and resveratrol enhanced the process, resulting in improved survivability of the embryos.

show abstract

Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability

Cited by 96 publications

References 53 publications

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

Effects of resveratrol treatment on mitochondria and subsequent embryonic development of bovine blastocysts cryopreserved by slow freezing

Contact Info

Product

Resources

About