Effect of Curcumin in Comparison with Trichostatin A on the Reactivation of Estrogen Receptor Alpha gene Expression, Cell Growth Inhibition and Apoptosis Induction in Hepatocellular Carcinoma Hepa 1-6 Cell lLine

Abstract

in the promoter region of TSGs (Yang et al., 2017). Abnormal regional hypermethylation of the 5′ region of several TSGs has been observed in HCC (Baylin et al., 2011). Down-regulation of a variety of TSGs expression because of abnormal hypermethylation in their promoter regions could play a critical role in liver tumorigenesis

Effect of DNA Methyltransferase in Comparison to and in Combination with Histone Deacetylase Inhibitors on Hepatocellular Carcinoma HepG2 Cell Line

“…In this experiment, We did not perform western blot in order to evaluate of HDAC1 and HDAC2 expression and also the acetylation status of histone H3 and a-tubulin but we decide to perform them in the next work. Previously, we evaluated the effect of GE and VPA on the other HCC cell lines including PLC/PRF5 and HepG 2 (Sanaei et al, 2017a;Kavoosi et al, 2016;Sanaei et al, 2017b). Thus, our studies provide a new avenue for cancer treatment through demethylating agents combined with histone deacethylase inhibitors.…”

“…Our previous studies clearly show that genistein has a significant inhibitory effect on the growth of hepatocellular carcinoma HepG2 cell lines and induces apoptosis in these cell line with a time-dependent manner (Sanaei et al, 2017a;Sanaei et al, 2017b).…”

Phytoestrogens in NAFLD: Potential Mechanisms of Action

“…Sanaei et al found that DNA demethylating agents such as genistein and histone deacetylase inhibitors (HDACIs) such as trichostatin A (TSA) may strongly reactivate silenced genes, enhance estrogen receptor alpha (ERα) reactivation and induction of apoptosis. These results may provide new avenues and therapeutic strategies for cancer therapy [48].…”

Histone Deacetylase Inhibitors in the Treatment of Hepatocellular Carcinoma: Current Evidence and Future Opportunities

“…Additionally, they conducted a study examining the efficacy of curcumin compared to TSA in the reactivation of ERa. They showed that both drugs can induce apoptosis and inhibit cell growth via reactivation of ERa [ 76 ].…”