Effect of curcumin extract against oxidative stress on both structure and deformation capability of red blood cell

Yang, Qinqin; Noviana, Milody; Zhao, Yajin; Chen, Dong; Wang, Xiang

doi:10.1016/j.jbiomech.2019.07.045

Cited by 10 publications

(6 citation statements)

References 43 publications

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“…Interestingly, the dose-response curve demonstrated that the IC 50 value of the investigated extract (25.3 μg/ml) was more potent than that of vitamin C (47.3 μg/ ml). Polyphenols, as previously established, protect against lipid peroxidation and cell membrane damage caused by oxidative stress [85,86]. Consequently, the obtained results indicated that the higher polyphenol content of the tested extract serves as a more efective lipid peroxidation inhibitor.…”

Section: Infuence Of the Stevia Extract On Tbars Developmentsupporting

confidence: 58%

Stevia rebaudiana Methanolic Leaf Extract in Egypt: Phytochemical Analysis, Antioxidant, Antilipid Peroxidation, Antihemolytic, Antimetastatic, and Anticancer Properties

Hanna

Osailan

Ahmed

2023

Journal of Food Biochemistry

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The phenolic and flavonoid content of the Stevia rebaudiana hydromethanolic extract (SRHME) was examined, and phytochemical identification using GC-mass spectrometry was achieved. Also, the antioxidant, antihemolytic, and antilipid peroxidation capabilities of the extract were assessed. The extract’s potential to induce apoptosis, inhibit cell proliferation, and reduce metastasis in SKVO3 cells was also checked. The findings of the GC-MS chromatogram demonstrated the existence of bioactive antioxidants and anticancer components in SRHME. Moreover, the extract demonstrated protection against cellular oxidative damage in human erythrocytes by preventing lipid peroxidation and hemolysis. Besides, SRHME demonstrated a selective cytotoxic effect with a strong IC50 value (17.5 µg/ml) on SKVO3 cells without any harmful effects on normal WI-38 cells using the MTT and LDH tests. Additionally, according to assays for wound healing and transwell chamber, the studied extract suppressed the ability of SKOV3 cells from migrating and invading, respectively. Also, the extract-treated SKVO3 cells showed rise in the percentage of apoptotic cells with a prominent comet nucleus, according to apoptotic assays in comparison to untreated cells. Furthermore, a flow cytometry analysis of SRHME-treated SKVO3 cells showed a halt in the S phase and an increase in sub-G1 apoptotic cells (25.44%). Also, the tested extract significantly decreased the levels of ROS in the treated cells, indicating that ROS was involved in the production of SKVO3 apoptosis. Lastly, SRHME strongly impacted the expression levels of proteins related to apoptosis, S-phase cell cycle arrest, and antimetastatic capacity in the treated SKVO3 cells.

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Section: Infuence Of the Stevia Extract On Tbars Developmentsupporting

confidence: 58%

Stevia rebaudiana Methanolic Leaf Extract in Egypt: Phytochemical Analysis, Antioxidant, Antilipid Peroxidation, Antihemolytic, Antimetastatic, and Anticancer Properties

Hanna

Osailan

Ahmed

2023

Journal of Food Biochemistry

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“…On the other hand, pretreatment with curcumin ameliorated the deteriorated deformability of erythrocytes subjected to hydrogen peroxide overload. In the range of 1–20 µmol/L of curcumin, the protection depended on the increasing concentration of curcumin with the highest effect in the presence of 20 µmol/L of curcumin [ 103 ].…”

Section: Protection Of Erythrocyte Nak-atpase and Deformability By Nutritional Antioxidantsmentioning

confidence: 99%

Erythrocyte Deformability and Na,K-ATPase Activity in Various Pathophysiological Situations and Their Protection by Selected Nutritional Antioxidants in Humans

Radošinská

Vrbjar

2021

IJMS

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The physicochemical and functional properties of erythrocytes are worsened in a variety of diseases. Erythrocyte deformability refers to their ability to adjust their shape according to external forces exerted against them in the circulation. It is influenced by the functionality of the Na,K-ATPase enzyme, which is localized in their membranes. The proposed review is focused on knowledge regarding changes in erythrocyte Na,K-ATPase activity, and their impact on erythrocyte deformability in various pathophysiological situations observed exclusively in human studies, as well as on the potential erytroprotective effects of selected natural nutritional antioxidants. A clear link between the erythrocyte properties and the parameters of oxidative stress was observed. The undesirable consequences of oxidative stress on erythrocyte quality and hemorheology could be at least partially prevented by intake of diverse antioxidants occurring naturally in foodstuffs. Despite intensive research concerning the effect of antioxidants, only a small number of investigations on erythrocyte properties in humans is available in databases. It is worth shifting attention from animal and in vitro experiments and focusing more on antioxidant administration in human studies in order to establish what type of antioxidant, in what concentration, and in which individuals it may provide a beneficial effect on the human organism, by protecting erythrocyte properties.

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“…On the other hand, the capacity of polyphenols to act as free-radical scavengers and electron donors give them a protective effect against oxidative stress [ 48 ]. Polyphenols prevent lipid peroxidation and cell membrane damage caused by oxidative stress [ 49 , 50 ].…”

Section: Impact Of Infusion and Decoction Process On The Antioxidamentioning

confidence: 99%

Evaluation of Polyphenol Content and Antioxidant Capacity of Aqueous Extracts from Eight Medicinal Plants from Reunion Island: Protection against Oxidative Stress in Red Blood Cells and Preadipocytes

Checkouri

Reignier²,

Silva

et al. 2020

Antioxidants

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Background—Medicinal plants are traditionally used as infusions or decoctions for their antioxidant, anti-inflammatory, hypolipidemic and anti-diabetic properties. Purpose—The aim of the study was to define the polyphenol composition and to assess the antioxidant capacity of eight medicinal plants from Reunion Island referred to in the French Pharmacopeia, namely Aphloia theiformis, Ayapana triplinervis, Dodonaea viscosa, Hubertia ambavilla, Hypericum lanceolatum, Pelargonium x graveolens, Psiloxylon mauritianum and Syzygium cumini. Methods—Polyphenol content was assessed by biochemical assay and liquid chromatography coupled to mass spectrometry. Antioxidant capacity was assessed by measuring DPPH reduction and studying the protective effects of herbal preparation on red blood cells or preadipocytes exposed to oxidative stress. Results—Polyphenol content ranged from 25 to 143 mg gallic acid equivalent (GAE)/L for infusions and 35 to 205 mg GAE/L for decoctions. Liquid chromatography coupled to mass spectrometry analysis showed the presence of major bioactive polyphenols, such as quercetin, chlorogenic acid, procyanidin and mangiferin. Antioxidant capacity assessed by different tests, including DPPH and Human red blood cell (RBC) hemolysis of herbal preparations, demonstrated a dose-dependent effect whatever the extraction procedure. Our data suggest that decoction slightly improved polyphenol extraction as well as antioxidant capacity relative to the infusion mode of extraction (DPPH test). However, infusions displayed a better protective effect against oxidative stress-induced RBC hemolysis. Conclusion—Traditional preparations of medicinal plant aqueous extracts (infusions and decoctions) display antioxidant properties that limit oxidative stress in preadipocytes and red blood cells, supporting their use in the context of metabolic disease prevention and treatment.

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Effect of curcumin extract against oxidative stress on both structure and deformation capability of red blood cell

Cited by 10 publications

References 43 publications

Stevia rebaudiana Methanolic Leaf Extract in Egypt: Phytochemical Analysis, Antioxidant, Antilipid Peroxidation, Antihemolytic, Antimetastatic, and Anticancer Properties

Stevia rebaudiana Methanolic Leaf Extract in Egypt: Phytochemical Analysis, Antioxidant, Antilipid Peroxidation, Antihemolytic, Antimetastatic, and Anticancer Properties

Erythrocyte Deformability and Na,K-ATPase Activity in Various Pathophysiological Situations and Their Protection by Selected Nutritional Antioxidants in Humans

Evaluation of Polyphenol Content and Antioxidant Capacity of Aqueous Extracts from Eight Medicinal Plants from Reunion Island: Protection against Oxidative Stress in Red Blood Cells and Preadipocytes

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