Effect of culture conditions on metabolites produced by the crinoid-derived fungus <i>Aspergillus ruber</i> 1017

Li, Yongfang; Xue, Wenrui; Niaz, Shah-Iram; Zhang, Liuhong; Huang, Zhijian; Lin, Yongcheng; Li, Jing; Liu, Lan

doi:10.1080/14786419.2016.1244200

Cited by 15 publications

(6 citation statements)

References 23 publications

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“…Aspergillus ruber produces large amounts of rubrocristin and erythroglaucin, giving the species its red colour ( Arai et al., 1989a , Wang et al., 2007b (misidentified as A . variecolor ), Li et al. 2017 ) in addition to asperinines, asperflavin, emodin, physcion, but the latter three are also produced by many other species in sect.…”

Section: Resultsmentioning

confidence: 99%

Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species

Houbraken¹,

Kocsubé²,

Visagie³

et al. 2020

Studies in Mycology

348

458

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The Eurotiales is a relatively large order of Ascomycetes with members frequently having positive and negative impact on human activities. Species within this order gain attention from various research fields such as food, indoor and medical mycology and biotechnology. In this article we give an overview of families and genera present in the Eurotiales and introduce an updated subgeneric, sectional and series classification for Aspergillus and Penicillium . Finally, a comprehensive list of accepted species in the Eurotiales is given. The classification of the Eurotiales at family and genus level is traditionally based on phenotypic characters, and this classification has since been challenged using sequence-based approaches. Here, we re-evaluated the relationships between families and genera of the Eurotiales using a nine-gene sequence dataset. Based on this analysis, the new family Penicillaginaceae is introduced and four known families are accepted: Aspergillaceae , Elaphomycetaceae , Thermoascaceae and Trichocomaceae . The Eurotiales includes 28 genera: 15 genera are accommodated in the Aspergillaceae ( Aspergillago , Aspergillus , Evansstolkia , Hamigera , Leiothecium , Monascus , Penicilliopsis , Penicillium , Phialomyces , Pseudohamigera , Pseudopenicillium , Sclerocleista , Warcupiella , Xerochrysium and Xeromyces ), eight in the Trichocomaceae ( Acidotalaromyces , Ascospirella , Dendrosphaera , Rasamsonia , Sagenomella , Talaromyces , Thermomyces , Trichocoma ), two in the Thermoascaceae ( Paecilomyces , Thermoascus ) and one in the Penicillaginaceae ( Penicillago ). The classification of the Elaphomycetaceae was not part of this study, but according to literature two genera are present in this family ( Elaphomyces and Pseudotulostoma ). The use of an infrageneric classification system has a long tradition in Aspergillus and Penicillium . Most recent taxonomic studies focused on the sectional l...

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Section: Resultsmentioning

confidence: 99%

Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species

Houbraken¹,

Kocsubé²,

Visagie³

et al. 2020

Studies in Mycology

348

458

View full text Add to dashboard Cite

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“…2,3-dihydroxybenzoic acid have previously been shown to possess S. aureus growth inhibitory activity [28] and should thus not be further investigated. (−)-Neoechinulin A, on the other hand, has been reported as inactive against S. aureus [29] while a hydroxylated analogue exhibited potent inhibitory activities [30]. Obtaining this knowledge directly from the crude extract allows for well-informed decision-making and emphasizes the potential of high-resolution growth inhibition profiling for expediting discovery of novel antibiotics from nature.…”

Section: High-resolution Staphylococcus Aureus Growth Inhibition Profmentioning

confidence: 99%

Coupling Microplate-Based Antibacterial Assay with Liquid Chromatography for High-Resolution Growth Inhibition Profiling of Crude Extracts: Validation and Proof-of-Concept Study with Staphylococcus aureus

Ardalani

Anam

Olsen³

et al. 2021

Molecules

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With the identification of novel antibiotics from nature being pivotal in the fight against human pathogenic bacteria, there is an urgent need for effective methodologies for expedited screening of crude extracts. Here we report the development and validation of a simple and dye-free antimicrobial assay in 96-well microplate format, for both determination of IC50 values and high-resolution inhibition profiling to allow pin-pointing of bioactive constituents directly from crude extracts. While commonly used antimicrobial assays visualize cell viability using dyes, the developed and validated assay conveniently uses OD600 measurements directly on the fermentation broth. The assay was validated with an investigation of the inhibitory activity of DMSO against Staphylococcus aureus, temperature robustness, interference by coloured crude extracts as well as inter-day reproducibility. The potential for high-resolution S. aureus growth inhibition profiling was evaluated on a crude extract of an inactive Alternaria sp., spiked with ciprofloxacin.

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“…in solid rice medium, for example, it generates extracts with antimicrobial potential against E. coli, S. aureus, Bacillus subtilis, Proteus mirabilis and Malassezia furfur (Chagas et al, 2017). Indeed, solid fermentation allows the production of a range of bioactive compounds (Li et al, 2016) such as fusarialin produced by Fusarium sp., with inhibitory activity against S. aureus in rice, in the study by Tchoukoua et al (2018).…”

Section: Antimicrobial Activity Of Raw Extractsmentioning

confidence: 99%

Evaluation of culture media and conditions of Amazonian filamentous fungi in an antimicrobial screening program

Negreiros

Guedes

Rodrigues

et al. 2021

RSD

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The rediscovery of bioactive compounds is a problem within natural product screening programs, because the chemical-genetic diversity of fungi is little explored and the standardization of cultivation conditions that allow obtaining new actives is critical in such programs. In this work, the impact of two solid mediums (rice and oats), a liquid medium (Czapeck broth) and different fermentation conditions were evaluated in order to explore new metabolic routes. Twelve filamentous fungi from Amazonian environments were used. UV-Vis spectrophotometry estimated the complexity of the extracts produced. The antimicrobial activity of the extracts was evaluated against an isolate of each Escherichia coli strain, Salmonella sp. and Staphylococcus aureus. Solid media proved to be more promising, as they allowed a wider range of active metabolites to be obtained. The oat medium provided a greater variety of metabolites, but due to the great complexity of the extracts obtained, the separation procedures were considerably more complex than for rice. Together, the rice culture medium and the use of 39 days of fermentation proved to be more promising conditions than the liquid medium normally used in screening programs in Brazil. The cultivation of Penicillium maximae (isolated for the second time in Brazilian territory) in solid medium provided the production of active fractions against E. coli in bioautography. In this study, it was observed that different fermentation conditions in solid culture are considerably promising in the search for bioactive natural products.

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Effect of culture conditions on metabolites produced by the crinoid-derived fungus Aspergillus ruber 1017

Cited by 15 publications

References 23 publications

Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species

Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species

Coupling Microplate-Based Antibacterial Assay with Liquid Chromatography for High-Resolution Growth Inhibition Profiling of Crude Extracts: Validation and Proof-of-Concept Study with Staphylococcus aureus

Evaluation of culture media and conditions of Amazonian filamentous fungi in an antimicrobial screening program

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