Effect of Cu2+-ascorbic acid on lipid peroxidation, Mg2+-ATPase activity and spectrin of RBC membrane and reversal by erythropoietin

Abstract

The effect of erythropoietin (Ep), a glycoprotein hormone, has been studied on lipid peroxidation induced by Cu2+ and ascorbate in vitro, Mg2+ ATPase activity and spectrin of RBC membrane. Our present investigation reveals that Cu2+ and ascorbic acid increases lipid peroxidation of RBC membrane significantly. It has further been observed that under the same experimental condition spectrin, a major cytoskeleton membrane protein, and Mg(2+)-ATPase activity of RBC membrane decrease significantly. However, exogenous administration of Ep completely restores lipid peroxidation and Mg(2+)-ATPase activity and partially recovers spectrin of RBC membrane.

Melatonin protects against gastric ulceration and increases the efficacy of ranitidine and omeprazole in reducing gastric damage

“…In an attempt to confirm whether melatonin directly scavenges · OH, an in vitro study was carried out using an established · OH generating system [36]. Fig.…”

“…To examine whether melatonin scavenges · OH in an in vitro system, · OH were generated for 1 hr in a system containing sodium phosphate buffer (50 mM; pH 7.4), ascorbate (1 mM) and 0.2 mM Cu 2+ [36, 37] in the absence or presence of DMSO (500 μ M) and different concentrations of melatonin in a final volume of 1 mL. For melatonin experiments, controls with ethanol were always included as the solution was prepared in the presence of ethanol, which scavenges · OH.…”

Interaction of flavonoids with red blood cell membrane lipids and proteins: Antioxidant and antihemolytic effects

“…For the preparation of hemoglobin-free ghosts, a method slightly modified from that of Dodge et al was followed [23][24][25]. Hemolysis of the washed red blood cells was performed in 5 mM sodium phosphate buffer (pH 8.0) and pellets were obtained by centrifugation at 13500 rpm for 30 min at 0-4 • C. This process was repeated five to seven times until the pellet became colorless.…”

Elephantopus scaber Linn. extract protects against copper ascorbate induced injury to goat heart mitochondria in-vitro: Involvement of antioxidant mechanisms

“…The incubation mixture containing mitochondrial membrane protein (1.6 mg/ml), 50 mM potassium phosphate buffer (pH 7.4), and 0.2mM Cu2+ and 1 mM ascorbic acid in a final volume of 1.0 ml was incubated at 37°C in an incubator for 1 hour. The reaction was terminated by the addition of 40 μl of 35 mM EDTA (Chattopadhyay et al, 1992).…”