Interaction of flavonoids with red blood cell membrane lipids and proteins: Antioxidant and antihemolytic effects

Chaudhuri, Sudip; Banerjee, Anwesha; Basu, Kaushik; Sengupta, Bidisa; Sengupta, Pradeep K.

doi:10.1016/j.ijbiomac.2006.12.003

Cited by 237 publications

(141 citation statements)

References 38 publications

(57 reference statements)

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“…To study the effect of herbal extracts against oxidative damage to lipids and proteins in human erythrocytes, free radical initiators, AAPH and APHZ, were employed for inducing oxidative stress. AAPH was source of free radical formation capable of inducing oxidation of lipid and protein structurally located on erythrocyte membranes (Chaudhuri et al, 2007). The results showed that erythrocytes were more resistant to AAPH-induced oxidation when herbal extracts were added, most of them had very strong inhibition effect.…”

Section: Resultsmentioning

confidence: 98%

Antioxidants in Thai Herb, Vegetable and Fruit Inhibit Hemolysis and Heinz Body Formation in Human Erythrocytes

Sangkitikomol¹

2012

Oxidative Stress - Environmental Induction and Dietary Antioxidants

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Section: Resultsmentioning

confidence: 98%

Antioxidants in Thai Herb, Vegetable and Fruit Inhibit Hemolysis and Heinz Body Formation in Human Erythrocytes

Sangkitikomol¹

2012

Oxidative Stress - Environmental Induction and Dietary Antioxidants

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“…F. gummosa stem extract showed better antihemolytic activity than the others (IC 50 was 277± 11.4 vs. 235± 9.1 µg ml -1 for vitamin C,). The antihemolytic activity of quercetin and other flavonoid have been previously reported and good activity of the extracts maybe result in high flavonoid content especially quercetin (Chaudhuri et al 2007). …”

Section: Seyed Fazel Nabavi Mohammad Ali Ebrahimzadeh Seyed Mohammamentioning

confidence: 93%

Antioxidant activity of flower, stem and leaf extracts of <i>Ferula gummosa</i> Boiss

Ebrahimzadeh¹,

Nabavi²,

Nabavi³

et al. 2010

Grasas y Aceites

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The Antioxidant and antihemolytic activities of hydroalcoholic extracts of the flowers, stems and leaves of the Ferula gummosa Boiss were investigated employing different in vitro assay systems. Leaf extract showed better activity in DPPH radical scavenging. In addition it showed better activity in nitric oxide and H₂O₂ scavenging and Fe²⁺ chelating activity than the other parts. The extracts exhibited good antioxidant activity in linoleic acid system test but were not comparable with vitamin C (p< 0.001). The extracts showed weak reducing power activity. The F. gummosa stem extract showed better antihemolytic activity against H²O² induced hemolysis. Among the extracts, the flowers had higher phenolic and flavonoid contents. This plant is very promising for further biochemical experiments.

La actividad antihemolítica y antioxidante de extractos hidroalcohólicos de flores, tallos y hojas de Ferula gummosa Boiss fueron investigados empleando diferentes ensayos in vitro. Los extractos de hojas mostraron una mejor actividad captadora de radicales libres. Además, mostraron una mejor actividad captadora de óxido nítrico y H₂O₂ y actividad quelatante de Fe²⁺ que las otras partes. Los extractos exhibieron una buena actividad antioxidante en el ensayo con ácido linoleico pero no comparable con la vitamina C (p< 0.001). Los extractos mostraron una débil actividad de poder reductor. Los extractos de tallos de F. gummosa mostraron una mejor actividad antihemolítica contra la hemolisis inducida con H₂O₂. Entre los extractos, las flores tienen los más altos contenidos de fenoles y flavonoides. Esta planta es muy prometedora para futuros experimentos bioquímicos

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“…The kinetics of erythrocytes resistance to hemolysis was determined at 37C° by continuous monitoring of changes in 620 nm absorbance. The time to reach 50% of total hemolysis was determined (half hemolysis Time; HT 50 ).…”

Section: Antioxidant Capacity Of Red Blood Cells (Hemolysis Assay)mentioning

confidence: 99%

“…We measured the effect of treatment the mice with MHGE on whole blood resistance to free radical aggression calculated HT 50 values, which revealed a half time for hemolysis in all treated groups compared to the control group (Figure 13). For the group MOHE treated with (100mg/kg), the increase in HT 50 …”

Section: Total Antioxidant Of Red Blood Cells (Hemolysis Test)mentioning

confidence: 99%

“…And/or the difference in the degree of the penetrations of the flavonoid molecules in intact erythrocytes. 50 The behavior of these extracts against the radical attack is always difficult to explain because the RBCs are a complex matrix in which the substances pharmacologically evaluated could be involved in many reactions in the cell membrane. 51 The application of PMA for 6 h significantly increased the weight of ear edema.…”

Section: Dpphmentioning

confidence: 99%

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In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

Djarmouni¹,

Baghiani²,

Adjadj³

et al. 2018

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Introduction: Santolina chamaecyparissus L. is a small medicinal herb, cultivated in Europe, Asia and Africa due to the antihelmintic, antiseptic, anti spasmodic, bactericidal, fungicidal, digestive and vulnerary properties. Despite this, S. chamaecyparissus aerial part extractions have not been examined for antioxidant, antihemolytic and anti-inflammatory properties. Methods: S. chamaecyparissus aerial parts were extracted with solvents of varying polarity: methanol (crude) extract (CrE) chloroform extract (CHE), ethyl acetate extract (EAE), and aqueous extract (AE). The content of total phenolics, and flavonoids in all the extracts were determined with spectrophotometric methods. Both enzymatic and non-enzymatic methods were used to evaluate the antioxidant activity of the extracts. Antioxidant activity of all the extracts were investigated using free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH• and 2,2'-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid (ABTS) assay), capacity of the inhibition of linoleic acid peroxidation (β-carotene assay), chelation of metals (iron chelating assay) and inhibition of xanthine oxidase (XO) activity. To investigate antihemolytic activity of the extracts, the 2, 2,-azobis (2-amidinopropane) dihydrochloride (AAPH) assay was used to induce erythrocyte oxidative hemolysis. An in vivo approach was carried out on mice treated with the methanol extract at a dose of 100 mg/kg/day for 21 consecutive days, and one group was treated with vitamin C (vitamin C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Phorbol 12-myristate 13-acetate (PMA)-induced ear edema was used to investigate the antiinflammatory activity of methanolic extract in mice. Results: Among all the extracts analyzed, the EAE exhibited a higher phenolic and flavonoids content (373.83 ± 0.23 mg gallic acid/g, and 61.51±7.86 mg quercetin/g respectively), followed by CE and CHE. DPPH and ABTS scavenging assays showed that EAE exhibited the highest effect in the both assays, with an IC 50 of 0.01 mg/ml and 0.0085 mg/ml respectively. All extracts moderately inhibited linoleic acid oxidation, with 57 % inhibition. The CE had a considerable chelating activity on ferrous iron (IC 50 = 0.32 mg/ml). In the enzymatic method by xanthine oxidase, results demonstrated that EAE had the highest XO inhibitory effect on both XO activity and Cyt-c reduction with IC50= 0.052±0.0003 mg/ml and 0.057±0.0006 mg/ml, respectively followed by CHE and CE. In the cellular system, CrE and CHE showed a hemolysis effect with % hemolysis (62.81% ± 1.43), (62.71% ± 1.01). However, EAE provided protection against AAPH-induced hemolysis with 53.67% ± 0.97. The in vivo assay exhibited a significant decrease (79.56%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (71.95% and 59.16% respectively). The methanol extract clearly demonstrated anti-inflammatory effects...

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Interaction of flavonoids with red blood cell membrane lipids and proteins: Antioxidant and antihemolytic effects

Cited by 237 publications

References 38 publications

Antioxidants in Thai Herb, Vegetable and Fruit Inhibit Hemolysis and Heinz Body Formation in Human Erythrocytes

Antioxidants in Thai Herb, Vegetable and Fruit Inhibit Hemolysis and Heinz Body Formation in Human Erythrocytes

Antioxidant activity of flower, stem and leaf extracts of <i>Ferula gummosa</i> Boiss

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

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