Effect of Cu on Pigmentation and Survival of Pseudomonas stutzeri

Abstract

Pseudomonas stutzeri is an aerobic, green fluorescent bacterium which secretes green pigment in aerobic condition. In this article the release of bluish-green, luminous, water-soluble stain by P. stutzeri was discussed in presence of Cu metal in relation with the survival of P.stutzeri in nutrient medium. The activity of Pseudomonas stutzeri was monitored in provisions of colors of pigment with varying concentrations of Cu metal in petri dish. Results showed a variation in colors of pigment with different concentration of Cu metal. It was light green with water while colourless at high concentration of metal and blue with the moderate concentration of Cu. It was observed that high concentration of Cu results in the death of all microorganisms may be due to the reaction of Cu with oxygen or Cu responsible to decrease the BOD which ultimately affects the biosynthesis of pigment. No color of Cu with any life of Pseudomonas stutzeri at high dose also indicates that the Cu adsorbed on dead mass of bacterial strain. Less microbial life at low and moderate concentration of Cu with blue color reflects that Cu may be bonded with the pigment. It was concluded that Cu 2+ was bactericidal to the phytopathogenic bacterium Pseudomonas stutzeri in the micromolar range which inhibits the biosynthesis of pigment at high concentration and bacteria experience death. It was suggested that pigmentation property of Pseudomonas stutzeri can be used as a monitoring tool of contaminated soil by heavy metals.

Function of enzyme lactate dehydrogenase in relation with lipid and glucose in biotic and abiotic stresses in the seedlings of Vigna radiata

“…A loopful culture of isolated organism was spread on filter paper strip for the appearance of deep purple color. Other biochemical tests were performed for the identification of organism using QTS-NE Kit (Table 1) (DESTO -laboratories, Pakistan) (Hayat et al, 2012).…”