Effect of Cryopreservation on Olive (Olea europaea L.) Plant Regeneration via Somatic Embryogenesis

Bradaï, Fatiha; Sánchez-Romero, Carolina

doi:10.3390/plants10010034

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…Differences in the vigor of plants regenerated via somatic embryogenesis depending on the genotypic background have been previously reported in olive [19]. The better features observed in plantlets of the line P5 could be explained by differences in the previous maturation phase, which occurred with different efficiencies depending on the embryogenic line [19,20] and largely determines the final output of the somatic embryogenesis process and the quality of the regenerated plants [21].…”

Section: Discussionmentioning

confidence: 63%

Effect of Cryopreservation on the Ex Vitro Establishment of Olive Plants Regenerated via Somatic Embryogenesis

Bradaï

Sánchez-Romero

2021

Plants

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Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should be investigated. The objective of this investigation was to evaluate the effect of cryopreservation on the regeneration performance of olive embryogenic cultures as well as on the quality of the plants obtained and their response to ex vitro establishment. In order to analyze the influence of the genotype, all the investigations were carried out in two genetically distinct embryogenic lines. The results obtained revealed no variation in the regeneration potential or the quality of the regenerated plants due to cryopreservation. The subsequent multiplication, rooting, and acclimatization steps were not influenced by cryopreservation either, although a significant genotype × cryopreservation interaction was found for shoot length during the multiplication step. The genotype played an important role, determining the quality of the regenerated plants and some aspects of the multiplication and rooting phases. This investigation revealed that the droplet-vitrification procedure optimized for the cryopreservation of olive somatic embryos can be efficiently used for the long-term conservation of olive embryogenic lines.

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Section: Discussionmentioning

confidence: 63%

Effect of Cryopreservation on the Ex Vitro Establishment of Olive Plants Regenerated via Somatic Embryogenesis

Bradaï

Sánchez-Romero

2021

Plants

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“…In general, regenerated plants display no morphological variation after cryopreservation ( Harding, 2004 ). For example, cryopreservation did not modify the regeneration potential or the quality of the regenerated plants in olive ( Bradaï and Sánchez-Romero, 2021 ), in grapevine ( Wang et al., 2002 ; Vasanth and Vivier, 2011 ), in paradise tree ( Melia azedarach L.) ( Scocchi et al., 2007 ), and in Citrus sp. ( Pérez et al., 1998 ).…”

Section: Genetic Stability Analysis In Cryopreserved Embryogenic Mate...mentioning

confidence: 99%

Current status of the cryopreservation of embryogenic material of woody species

Ballesteros,

Martínez,

Sánchez-Romero

et al. 2024

Front. Plant Sci.

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Cryopreservation, or the storage at liquid nitrogen temperatures (-196°C), of embryogenic cells or somatic embryos allows their long-term conservation without loss of their embryogenic capacity. During the last decade, protocols for cryopreservation of embryogenic material of woody species have been increasing in number and importance. However, despite the large experimental evidence proved in thousands of embryogenic lines, the application for the large-scale conservation of embryogenic material in cryobanks is still limited. Cryopreservation facilitates the management of embryogenic lines, reducing costs and time spent on their maintenance, thus limiting the risk of the appearance of somaclonal variation or contamination. Somatic embryogenesis in combination with cryopreservation is especially useful to preserve the juvenility of lines while the corresponding clones are being field-tested. Hence, when tree performance has been evaluated, selected varieties can be propagated from the cryostock. The traditional method of slow cooling or techniques based on vitrification are mostly applied procedures. For example, slow cooling methods are widely applied to conserve embryogenic lines of conifers. Desiccation based procedures, although simpler, have been applied in a smaller number of species. Genetic stability of the cryopreserved material is supported by multiloci PCR-derived markers in most of the assayed species, whereas DNA methylation status assays showed that cryopreservation might induce some changes that were also observed after prolonged subculture of the embryogenic lines. This article reviews the cryopreservation of embryogenic cultures in conifers, fruit species, deciduous forest species and palms, including a description of the different cryopreservation procedures and the analysis of their genetic stability after storage in liquid nitrogen.

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“…Bradaï and Sánchez-Romero [41] evaluated the effect of cryopreservation on the different phases of the somatic embryogenesis process. For this purpose, the behavior of cultures established from cryopreserved somatic embryos was compared with that of control, non-cryopreserved cultures during the proliferation, maturation, and germination steps.…”

Section: Somatic Embryogenesis In Olivementioning

confidence: 99%

“…Though different somatic embryogenesis protocols are available in olive [20], their efficiency varies depending on the genotype [20,22,41]. This influence of the genetic constitution has been observed in the different phases of this multi-step technique, from the initiation of embryogenic cultures [21,43] to the germination of somatic embryos [40].…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Somatic Embryogenesis in Olive

Sánchez-Romero

2021

Plants

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The olive is a fruit tree species economically very important in countries of the Mediterranean basin. Somatic embryogenesis is a powerful in vitro technique with multiple applications in different fields, including breeding programs performed by both classical and innovative procedures. This editorial paper presents a special issue focused on “Somatic embryogenesis in olive”. In this manuscript, the conceptual framework of the special issue is established and the contributions are summarized and put into context. Finally, the main bottlenecks limiting the practical applicability of somatic embryogenesis are identified and the future research prospects are discussed.

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Effect of Cryopreservation on Olive (Olea europaea L.) Plant Regeneration via Somatic Embryogenesis

Cited by 5 publications

References 34 publications

Effect of Cryopreservation on the Ex Vitro Establishment of Olive Plants Regenerated via Somatic Embryogenesis

Effect of Cryopreservation on the Ex Vitro Establishment of Olive Plants Regenerated via Somatic Embryogenesis

Current status of the cryopreservation of embryogenic material of woody species

Somatic Embryogenesis in Olive

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