Effect of Cry-consensus Peptide, a Novel Recombinant Peptide for Immunotherapy of Japanese Cedar Pollinosis, on an Experimental Allergic Rhinitis Model in B10.S Mice

Abstract

It was concluded that Cry-consensus peptide effectively controlled allergic responses, which results from shifting from a Th2-dominated to a Th1-dominated immune response.

A new murine model of allergic rhinitis by repeated intranasal Cry j 1 challenge

“…This model of allergic rhinitis produces sneezing, eosinophil infiltration into the nasal mucosa, and Cry j 1-specific IgE production, and should prove useful in studying human cedar pollinosis. We also examined the efficacy of an immunotherapeutic peptide in this model (7,17). However, this model and others only mimic short-term rhinitis and not seasonal rhinitis.…”

“…To study the relevant immunological mechanisms of Cj pollinosis and establish new therapeutic approaches, we had previously developed an allergic rhinitis model in B10.S mice elicited by histamine pretreatment and continuous intranasal antigen challenges for 5 days (17). This model of allergic rhinitis produces sneezing, eosinophil infiltration into the nasal mucosa, and Cry j 1-specific IgE production, and should prove useful in studying human cedar pollinosis.…”

Anti-allergic potential of oligomannose-coated liposome-entrapped Cry j 1 as immunotherapy for Japanese cedar pollinosis in mice

“…Splenocytes from Cry j 1/OML-treated mice secreted much more IFN-γ than those from control mice in response to in vitro stimulation with the allergen, indicating that allergen-specific Th1 cells were induced in the treated mice. The IL4/IFN-γ and IL-5/IFN-γ ratios for cytokines secreted from spleen cells, which reflect the Th1/Th2 balance [20,21], were also lower in the Cry j 1/OML-treated mice than in PBS-and Cry j 1/alumtreated mice. Since the level of IgE is positively regulated by Th2 cytokines [19], repression of serum IgE elevation in Cry j 1/OMLtreated mice may be due to Th1-skewing of the immune response.…”

“…Repeated subcutaneous administration of Cry-consensus peptide inhibited the production of anti-Cry j 1 IgE and significantly enhanced anti-Cry j 1 IgG2a [20,21]. However, a higher dose (1 mg/mouse) of the peptide was required to suppress IgE levels and to induce a Th1 immune response.…”

Solid-in-Oil Nanodispersions for Transcutaneous Immunotherapy of Japanese Cedar Pollinosis

“…T cell epitope peptide Epitope p246-259 of Cry j 2 Oral Suppression of Th1 and Th2 responses [20,21] Integrated peptide from three epitopes Oral Inhibition of lymph node cell proliferation to epitopes from Cry j 1/2 [22] Rice seed containing peptides from fourteen epitopes Oral - [23] Rice seed containing 7Crp from seven epitopes Oral Inhibition of T-cell proliferative response to Cry j 1 [24] Chicken egg containing 7Crp from seven epitopes Oral Induction of oral tolerance [25] Cry-consensus from five or six epitopes SC Induction of IgG2a, suppression of ratios of IL-4/IFN-γ and IL-5/IFN-γ,Th1 deviation [17,26] 7Crp from seven epitopes SL Induction of IL-10-producing regulatory T cells [27] 7CrpR from seven epitopes T - [28] Mixture of seven epitopes T Suppression of Th1 and Th2 responses [29] [46] 7CrpR from seven epitopes combined with R848 T Induction of IgG 2a/IgE ratio, Th1 deviation [47] ID: intradermal, IM: intramuscular, SC: subcutaneous, SL: sublingual, T: transcutaneous.…”