Effect of cooking methods on protein content and neurotoxin (β-ODAP) concentration in grass pea (<i>Lathyrus sativus</i> L.) grains

Barpete, Surendra; Gupta, Priyanka; Khawar, Khalid Mahmood; Kumar, Shiv

doi:10.1080/19476337.2021.1915879

Cited by 11 publications

(7 citation statements)

References 31 publications

(48 reference statements)

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“…However, it has been demonstrated that they can be eliminated by various processing techniques. The above mentioned non-proteinogenic amino acids in Senegalia seeds, except the sulfur-containing ones (i.e., S-carboxyethylcysteine, S-carboxyisopropylcysteine, γ-glutamyl-djenkolic acid) (Boughton et al, 2015), have been reported to exhibit high resistance to heating and are mainly eliminated through leaching following soaking and boiling processes (Akalu et al, 1998;Barpete et al, 2021;Riasi et al, 2014). Besides, it is speculated that cooking can reduce their toxicity by isomerization (Bell, 2003;Lambein et al, 2007).…”

Section: Non-proteinogenic Amino Acidsmentioning

confidence: 99%

Inventory of human-edible products from native Acacia sensu lato in Africa, America, and Asia: Spotlight on Senegalia seeds, overlooked wild legumes in the arid tropics

Drabo

Shumoy²,

Savadogo³

2022

Food Research International

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Section: Non-proteinogenic Amino Acidsmentioning

confidence: 99%

Inventory of human-edible products from native Acacia sensu lato in Africa, America, and Asia: Spotlight on Senegalia seeds, overlooked wild legumes in the arid tropics

Drabo

Shumoy²,

Savadogo³

2022

Food Research International

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“…Overnight soaking and then boiling next day. (114,(132)(133)(134)(135) Soaking in alkaline water and boiling…”

Section: Boiling In Freshwatermentioning

confidence: 99%

“…Soaking seeds overnight followed by autoclaving/ pressure cooked at 15 psi for different time intervals, say 15, 30, and 45 min. (114,132,135) Frying Overnight soaking and then deep frying in vegetable oil. (132) Fermentation with bacterial and fungal inoculum Overnight soaking of seeds followed by boiling and then it is crushed in a mixer for 5 s before being placed on Petri dishes.…”

Section: Autoclavingmentioning

confidence: 99%

“…Mutation studies on grasspea with gamma-rays are encouraging and have shown induced salinity (NaCl) tolerance in M2 progeny mutants of grasspea ( 112 ). Different kinds of auxins (IBA; IAA; NAA) in tissue culture experiments are carried out to find the factors affecting healthy rooting, acclimatization and the effects of different concentration of sugar on root morphology phenology and developmental attributes of grasspea plants ( 25 , 113 , 114 ).…”

Section: Breeding Efforts For Nutritional Gain In Grasspeamentioning

confidence: 99%

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Rediscovering the Potential of Multifaceted Orphan Legume Grasspea- a Sustainable Resource With High Nutritional Values

et al. 2022

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The genus Lathyrus consists of more than 184 herbaceous annual and perennial species suitable for multifaceted sustainable food and feed production system in the arid and semi-arid regions of the world. The grasspea is a promising source of protein nutrition. However, its potential is not being utilized fully due to the presence of neurotoxin content (β-N-oxalyl-l-α, β diaminopropionic acid, β-ODAP), a causal agent of non-reversible lower limbs paralysis. The high protein contents in seeds and leaves with ~90% digestibility make it sustainable super food to beat protein malnutrition in future. Therefore, it is desired to breed new grasspea cultivars with low β-ODAP contents. Limited research has been carried out to date about this feature. A draft genome sequence of grasspea has been recently published that is expected to play a vital role in breeding and identifying the genes responsible for biosynthesis pathway of β-ODAP contents in grasspea. Efforts to increase awareness about the importance of genus Lathyrus and detoxify β-ODAP in grasspea are desired and are in progress. Presently, in South Asia, systematic and dedicated efforts to support the farmers in the grasspea growing regions by disseminating low β-ODAP varieties has resulted in a considerable improvement in reducing the incidence of neurolathyrism. It is expected that the situation will improve further by mainstreaming grasspea cultivation by implementing different approaches such as the development and use of low β-ODAP varieties, strengthening government policies and improved detox methods. The present review provides insight into the multifaceted characteristics of sustainable nutritious grasspea in the global and Indian perspective.

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“…In vitro tissue culture techniques have excellent potential to enhance and improve the agronomical traits through creation of somaclonal variation, screening for salt and drought tolerant genotypes, identification of micronutrient concentrations/toxicity, generation advancement or regeneration of true to the type plant in grass pea (Barpete et al, 2016(Barpete et al, , 2020a(Barpete et al, , 2021. However, grass pea is highly recalcitrant and difficult to regenerate under in vitro conditions (Barpete et al, 2016).…”

Section: Improvement Via In Vitro and Transgenic Technologymentioning

confidence: 99%

Current Perspectives on Reducing the β-ODAP Content and Improving Potential Agronomic Traits in Grass Pea (Lathyrus sativus L.)

Das

Parihar

Barpete³

et al. 2021

Front. Plant Sci.

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Grass pea is well-established as one of the most resilient and versatile crops that can thrive under extreme climatic circumstances such as cold, heat, drought, salt-affected soils, submergence, and excessive rainfall along with resistance to several diseases and pests. However, despite the awareness of its virtues, its cultivation globally has decreased recently owing to the presence of a neurotoxin, β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP), in the seedlings and seeds of this legume, which has been reported to cause neurolathyrism, a non-reversible neurological disorder in humans and animals. Significant repositories of Lathyrus germplasm are available across countries that have provided access to a wide range of agro-morphological traits as well as the low β ODAP content. Efforts have been made worldwide to use these germplasms for the genetic enhancement of grass pea to make this food safe for human consumption. Efforts on molecular breeding of this crop are also lagging. However, during the last decade, the research scenario has changed with some efforts being made toward improving this climate resilient pulse in terms of genomic resources. Molecular markers have also been used to evaluate the interspecific diversity as well as the phylogenetic relationship among the species and mapping studies. Intron-targeted amplified polymorphic, genomic simple sequence repeat, resistance genes analogs, and disease resistance markers developed for other legume species have been successfully cross-amplified in grass pea. Transcriptomic studies have recently been undertaken on grass pea by deploying several second-generation sequencing techniques. In addition, a few studies have attempted to unveil the genes and the underlying mechanism conferring biotic and abiotic stress or regulating the pathway of β-ODAP in grass pea. Proteomics has accelerated the identification studies on differential proteomes in response to salinity and low-temperature stress conditions for unveiling the common signaling pathways involved in mitigating these abiotic stresses and in discovering differentially regulated proteins. In grass pea, a metabolomics approach has been used to identify the metabolic processes associated with β-ODAP synthesis. Genome sequencing of grass pea is under way which is expected to be vital for whole-genome re-sequencing and gene annotation toward the identification of genes with novel functions. Recently, a draft genome sequence of grass pea was developed, and some efforts are underway to re-sequence a diverse panel of grass pea comprising 384 germplasm lines. Owing to the scantiness of a successful transformation protocol, research on the application of modern approaches of genome editing like the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) or CRISPR-associated protein 9 (CRISPR/Cas9) system for the engineering of signaling pathways or regulatory mechanisms seeks immediate attention to reduce the β-ODAP content in seeds and to improve the potential agronomic traits in grass pea.

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Effect of cooking methods on protein content and neurotoxin (β-ODAP) concentration in grass pea (Lathyrus sativus L.) grains

Cited by 11 publications

References 31 publications

Inventory of human-edible products from native Acacia sensu lato in Africa, America, and Asia: Spotlight on Senegalia seeds, overlooked wild legumes in the arid tropics

Inventory of human-edible products from native Acacia sensu lato in Africa, America, and Asia: Spotlight on Senegalia seeds, overlooked wild legumes in the arid tropics

Rediscovering the Potential of Multifaceted Orphan Legume Grasspea- a Sustainable Resource With High Nutritional Values

Current Perspectives on Reducing the β-ODAP Content and Improving Potential Agronomic Traits in Grass Pea (Lathyrus sativus L.)

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