Effect of controlled release of fibroblast growth factor‐2 from chitosan/fucoidan micro complex‐hydrogel on in vitro and in vivo vascularization

Abstract

We produced a chitosan/fucoidan micro complex-hydrogel as a carrier for controlled release of heparin binding growth factors such as fibroblast growth factor (FGF)-2. Material consisting of a soluble chitosan (CH-LA) mixed with fucoidan yielded a water-insoluble and injectable hydrogel with filamentous particles. In this study, we examined the ability of the chitosan/fucoidan complex-hydrogel to immobilize FGF-2 and to protect its activity, as well as the controlled release of FGF-2 molecules. The chitosan/fucoidan complex-hydrogel has high affinity for FGF-2 (K(d) = 5.4 x 10(-) (9)M). The interaction of FGF-2 with chitosan/fucoidan complex-hydrogel substantially prolonged the biological half-life time of FGF-2. It also protected FGF-2 from inactivation, for example by heat and proteolysis, and enhance FGF-2 activity. When FGF-2-containing complex-hydrogel was subcutaneously injected into the back of mice, significant neovascularization and fibrous tissue formation were induced near the site of injection at 1 week, and the complex-hydrogel was biodegraded and disappeared by 4 weeks. These findings indicate that controlled release of biologically active FGF-2 molecules is caused by both slow diffusion and biodegradation of the complex-hydrogel, and that subsequent induction of vascularization occurs. FGF-2-containing chitosan/fucoidan micro complex-hydrogel is thus useful and convenient for treatment of ischemic disease.

Application of BCBB/BMP/bFGF Complex in Repairing Femoral Head Necrosis in Rabbit Models

“…BMP can induce MSC differentiation into chondrocytes and osteoblasts 6,15) , and bFGF promotes the proliferation of differentiated MSCs 7,16,17) . At 4 w, 8 w, and 12 w after operation, new bone formation area in Group C was larger than that in Group B, which was larger than Group A, with significance (P < 0.05).…”

“…The bFGF is a powerful angiogenic growth factor 7,20) . In this study, anatomical observation showed that at 2 w and 4 w after operation, fresh tissues grew actively.…”

“…With the development of tissue engineering, the application of bone tissue engineering technique may be a breakthrough for FHN repairing 4) . Bone morphogenetic protein (BMP) and basic fibroblast growth factor (bFGF) have been well studied in recent years [6][7][8] . BMP can induce mesenchymal stem cells (MSCs) to differentiate into chondrocytes and osteoblasts 9,10) .…”

New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation

“…All the statistical analyses were carried out with GraphPad Prism Version 5.0 software. The integration of growth factors (GFs) in controlled release systems is a useful and much-explored method to improve their efficacy in vivo [28][29][30][31][32]. However, it is assumed that inducing the repair of complex tissues, such as cartilage, might only be possible through the orchestrated co-delivery of two or more GFs.…”

Effects of attached bacteria on organic aggregate settling velocity in seawater

“…The model aggregates were prepared using fucoidan (a sulfated polysaccharide found in brown seaweed) and chitosan (a partially deacetylated derivative of the amino polysaccharide, chitin) according to a gel preparation protocol originally developed by Nakamura et al (2008) for pharmaceutical purposes. Fucoidan was extracted from Kjellmanialla crassifolia (Gagomekombu) according to Nakamura et al (2008). Gagome-kombu powder (10 g) (Toyonaka Matumae Konbu Honpo Corp., Osaka, www.kobuya.net) was added to 100 ml 70% by volume ratio (v/v) ethanol containing 0.1 M acetic acid in a 200 ml conical glass flask.…”