Effect of contact angle, zeta potential and particles size on the in vitro studies of Al ₂ O ₃ and SiO ₂ nanoparticles

Abstract

Currently, nanometal oxides find their role in different biological applications such as tissue engineering, implant and bone replacement materials. Owing to the increased use of nanoparticles, it is necessary to understand their release and toxicity in the biological system. In this regard, three independent studies such as in vitro cytotoxicity, antioxidant activity and biocompatibility of nano- and micrometal oxide particles such as alumina (Al2O3) and silica (SiO2) are evaluated. It is evident from cell viability study that nanoAl2O3 and SiO2 particles are less toxic when compared with microAl2O3 and SiO2 to NIH 3T3 cell lines up to 200 µg/ml. Antioxidant properties of micro- and nanoAl2O3 in terms of radical scavenging percentage for micro- and nanoAl2O3 are 59.1% and 72.1%, respectively, at 100 mg. Similarly, the radical scavenging percentage of nano- and bulk SiO2 are 81.0% and 67.2%, respectively. The present study reveals that the cellular behaviour, interaction and biocompatibility of metal oxides differ with dose, particle size, contact angle and zeta potential. The present study opens up a new strategy to analyse in vitro nanotoxicity.

Comparison of graphene impregnated with/without nanosilver prepared by submerged arc discharge method

“…After the graphene-nanosilver composite and noncomposite colloids had been obtained through the corona discharge, their particle size distributions, zeta potentials, and optical properties were measured and compared. 17…”

Metal‐based nanoparticles for bone tissue engineering

“…On the other hand, decreasing microscale pore sizes to nanoscale sometimes became hazardous for cells viability. As Karunakaran, Suriyaprabha, Rajendran, and Kannan (2014)) study for micro‐ and nano‐SiO 2 and Al 2 O 3 cytotoxicity on NIH 3T3 cells illustrated that the antioxidant activity for the material was more pronounced nanoscale material in comparing with micro.…”

Tissue distribution following 28 day repeated oral administration of aluminum‐based nanoparticles with different properties and the in vitro toxicity

“…Most aluminum-containing compounds have a low solubility in water, and are not easily ionized, even in acidic conditions, such as gastric juice (pH 1.5-3.5) and the lysosomal lumen (pH 4.5-5.0) (Doshi, Braida, Christodoulatos, Wazne, & O 0 Connor, 2008;Zhang et al, 2013). Additionally, it is known that the toxicity of NPs depends on their properties both in the manufactured condition (primary) and within a biological system (secondary) (Karunakaran, Suriyaprabha, Rajendran, & Kannan, 2015;Radziun et al, 2011). However, knowledge regarding the relationship between the properties of AlONPs and their biodistribution is extremely limited.…”