Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells

Chujo, Hitomi; Yamasaki, Masao; Nou, Shinsuke; Koyanagi, Nami; Tachibana, Hirofumi; Yamada, Koji

doi:10.1016/s0304-3835(03)00478-6

Cited by 77 publications

(42 citation statements)

References 10 publications

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“…However, recent in vitro studies [13,18,19] showed that t10,c12-CLA is somewhat more efficacious than c9,t11-CLA for many parameters, suggesting the possibility that lower doses of t10,c12-CLA can be utilized in vivo, with good efficacy and low toxicity. The results in Fig.…”

Section: Discussionmentioning

confidence: 99%

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Beppu

Hosokawa

Tanaka

et al. 2006

The Journal of Nutritional Biochemistry

122

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This study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers (cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA) on human colon cancer cell lines (Caco-2, HT-29, and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 M each isomer even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA, and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+2.7% for t9,t11-CLA, 35.9+0.3% for c9,t11-CLA, and 46.3+0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was dose-and time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA.t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in association with apoptosis, whereas Bax levels remained unchanged. These results suggest that decreased expression of Bcl-2 by t9t11-CLA might increase the sensitivity of cells to lipid peroxidation and to programmed cell death, apoptosis. AbstractThis study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers (cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA) on human colon cancer cell lines (Caco-2, HT-29, and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 µM each isomer even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA, and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. * Manuscript 2CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+2.7% for t9,t11-CLA, 35.9+0.3% for c9,t11-CLA, and 46.3+0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was doseand time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA. t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in...

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Section: Discussionmentioning

confidence: 99%

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Beppu

Hosokawa

Tanaka

et al. 2006

The Journal of Nutritional Biochemistry

122

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“…It has been reported that a food component, for example, conjugated linoleic acid (CLA), inhibits E2-induced proliferation of MCF-7 cells. 11) Hence the effect of royal jelly on the growth-promotion activity of BPA was investigated. As indicated in Fig.…”

mentioning

confidence: 99%

Effect of Royal Jelly on Bisphenol A-Induced Proliferation of Human Breast Cancer Cells

Nakaya

Onda

Sasaki

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…In particular, it has long been recognized that CLA inhibits chemically induced carcinogenesis in tissues such as the mammary gland, skin, forestomach and intestine. Furthermore, CLA has been shown to inhibit the proliferation of hepatoma, lung, colon and breast cancer cells in vitro studies (Schonberg & Krokan 1995;Chujo et al 2003;Liu & Sidell 2005).…”

Section: Conjugated Linoleic Acidmentioning

confidence: 99%

Dairy Ingredients in New Functional Food Product Development

Amaya-Llano

Ozimek

2010

Functional Food Product Development

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Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells

Cited by 77 publications

References 10 publications

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Effect of Royal Jelly on Bisphenol A-Induced Proliferation of Human Breast Cancer Cells

Dairy Ingredients in New Functional Food Product Development

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