Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria

Grover, Harpreet Singh; Deswal, Himanshu; Hans, Veenu Madaan; Devi, Leimapokpam Sumitra

doi:10.4103/1658-6816.200138

Cited by 4 publications

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“…They have long been utilized to treat respiratory ailments. Many previous studies have shown the antimicrobial activity of ethanol extract from eucalyptus leaves against oral microorganisms due to the specific activity of 1,8-cineole against periodontopathic and cariogenic bacteria, such as Porphyromonas gingivalis, Fusobacterium nucleatum, S. mutans, and S. sobrinus (8,9).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Nonnutritive Sweeteners on the Antimicrobial Activity of Eucalyptus Extracts against Salivary Mutans Streptococci (in-vitro Study)

2022

ARI

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The eucalyptus tree is an excellent source of antimicrobial agents; it is used in many oral cure products. The bitter taste of these agents could compromise their usage. Therefore, fortifying the extracts with non-nutritive sweeteners could be a promising procedure for masking their unpleasant taste. This study was an in vitro evaluation of the antimicrobial activity of eucalyptus (alcoholic and aqueous) extracts against salivary Streptococci mutans. It aimed to investigate the effect of non-nutritive sweeteners on the antimicrobial activity of these extracts against salivary S. mutans. The test microbes were sensitive to different concentrations of eucalyptus alcoholic and aqueous extract, and the inhibition zone increased as the concentration of the extracts increased. All the Mutans isolates were killed at a concentration of 75 mg/ml for the alcoholic extract and 175 mg/ml for the aqueous extracts. In this experiment, the concentration of up to 15% stevia and up to 5% sucralose did not affect the antimicrobial activity of eucalyptus alcoholic extract. While the concentration of up to 1% of stevia and sucralose did not interfere with the antimicrobial activity of aqueous eucalyptus extract against salivary S. mutans. An increase in the concentration of non-nutritive sweeteners in this experiment appeared to interfere with the antimicrobial activity of eucalyptus extract against salivary S. mutans.

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Section: Introductionmentioning

confidence: 99%

The Effect of Nonnutritive Sweeteners on the Antimicrobial Activity of Eucalyptus Extracts against Salivary Mutans Streptococci (in-vitro Study)

2022

ARI

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Insights into the enhanced photocatalytic and antioxidant properties of novel biogenically synthesised β-In₂S₃ quantum dots

Mishra

Gadore

Ghotekar

et al. 2023

International Journal of Environmental Analytical Chemistry

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CharacterizationX-ray diffraction study of g-β-In2S3 was conducted using Philips X'PERT with Cu-Kα radiation with a scanning speed of 2 0 /minute at room temperature at around 25 0 C. Photoluminescence spectra were recorded from the Varian Cary eclipse fluorescence spectrophotometer. SEM analyses were done using FEI Quanta FEG 200-High Resolution Scanning Electron Microscope to evaluate the surface and topography of the quantum dots. To

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Evaluation of Antioxidant and Antimicrobial Efficacy of Camellia Sinensis and Alstonia Scholaris Extracts on Streptococcus Mutans and Lactobacillus Acidophilus - An in Vitro Study.

Goel

Chandan

2021

Biomed. Pharmacol. J.

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Context Dental caries is showing an upward trend in India and there is a need to explore innovative strategies to prevent the disease. Literature evaluating antibacterial activity of Camellia sinensis and Alstonia scholaris plant extracts on Streptococcus mutans and Lactobacillus acidophilus is practically non-existent. Objectives - To assess the minimum inhibitory concentration (MIC) and antimicrobial efficacy of Camellia sinensis and Alstonia scholaris on S. mutans and L. acidophilus. Materials and method: This was an in vitro study carried over a period of three months. The leaves of Camellia sinensis and Alstonia scholaris were collected, and crushed to obtain coarse powder. Plant extraction was performed using Soxhelet appartus. Anti- oxidant assay was performed for both the plant extracts against DPPH radical using Spectrophotometer at 517nm. Inhibition percentage was calculated through absorbance value measured from spectrophotometer. Anti- microbial activity of both the plant extracts against Microbial Type Culture Collection strains of Streptococcus mutans and Lactobacillus acidophilus was assessed using Agar well diffusion method. 0.2% Chlorhexidine was used as positive control and ethanol as negative control. The experiment was performed in triplicates. Mean inhibition zone in each set of experiment was computed using three readings after accounting for well diameter. One Way Analysis of Variance (ANOVA), Tukey’s post hoc test and independent sample‘t’ test were performed to compare the mean inhibition zone. Results: The plant extracts were effective against Streptococcus mutans and Lactobacillus acidophilus. Camellia sinensis at 4% concentration produced a mean inhibition zone of 30.3± 3.9 mm against Streptococcus mutans and 23.8± 2.2 mm against Lactobacillus acidophilus. Alstonia scholaris at 10% concentration produced a mean inhibition zone of 21.6± 2.8 mm against Streptococcus mutans and 24.1± 1.6 mm against Lactobacillus acidophilus. Conclusion: Camellia sinensis and Alstonia scholaris have significant anti- oxidant and anti- microbial property against Streptococcus mutans and Lactobacillus acidophilus.

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Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria

Cited by 4 publications

References 0 publications

The Effect of Nonnutritive Sweeteners on the Antimicrobial Activity of Eucalyptus Extracts against Salivary Mutans Streptococci (in-vitro Study)

The Effect of Nonnutritive Sweeteners on the Antimicrobial Activity of Eucalyptus Extracts against Salivary Mutans Streptococci (in-vitro Study)

Insights into the enhanced photocatalytic and antioxidant properties of novel biogenically synthesised β-In₂S₃ quantum dots

Evaluation of Antioxidant and Antimicrobial Efficacy of Camellia Sinensis and Alstonia Scholaris Extracts on Streptococcus Mutans and Lactobacillus Acidophilus - An in Vitro Study.

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Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria

Cited by 4 publications

References 0 publications

The Effect of Nonnutritive Sweeteners on the Antimicrobial Activity of Eucalyptus Extracts against Salivary Mutans Streptococci (in-vitro Study)

The Effect of Nonnutritive Sweeteners on the Antimicrobial Activity of Eucalyptus Extracts against Salivary Mutans Streptococci (in-vitro Study)

Insights into the enhanced photocatalytic and antioxidant properties of novel biogenically synthesised β-In2S3 quantum dots

Evaluation of Antioxidant and Antimicrobial Efficacy of Camellia Sinensis and Alstonia Scholaris Extracts on Streptococcus Mutans and Lactobacillus Acidophilus - An in Vitro Study.

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Product

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Insights into the enhanced photocatalytic and antioxidant properties of novel biogenically synthesised β-In₂S₃ quantum dots