Effect of combined application of bFGF and inorganic polyphosphate on bioactivities of osteoblasts and initial bone regeneration

Yuan, Quan; Kubo, Takayasu; Doi, Katsuhiko; Morita, Koji; Takeshita, Ryou; Katoh, Shinichi; Shiba, Toshikazu; Gong, Ping; Akagawa, Yasumasa

doi:10.1016/j.actbio.2009.01.034

Cited by 37 publications

(27 citation statements)

References 35 publications

(58 reference statements)

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“…In the result of histological evaluation, bone formation was detected inside pores at bottom portion of bone defect, but upper and central portion of IP-CHAs pore was hardly observed. Our previous studies performed in the bone socket of rabbit femurs, placed IP-CHA were filled newly bone at early periods 12,13) . These bone defects are able to be protected a mechanical stress and some negative factor for bone formation.…”

Section: Discussionmentioning

confidence: 99%

“…Because it was fabricated at a high temperature and hardly-absorbed, CaPO 4 mineral was not eluted and did not effect in vivo osteogenic differentiation 17) . In contrast, preadsorption of poly(P) onto HA promoted osteogenic differentiation of stem cells to facilitate the formation of new bone 9,10,12) . It is considered that Poly(P)/IP-CHA has ability both osteoconduction and osteroinduction in bone regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…High levels of new bone formation were observed in Poly(P)/IP-CHA. onto block type IP-CHA composites accelerated early bone regeneration in a rabbit femur model 12,13) . Blocks of bone graft material are difficult to apply for implant GBR because of the complex topography of the bone defect at the implant site.…”

Section: Discussionmentioning

confidence: 99%

“…On the basis of these preclinical studies suggesting that poly(P) promotes cell proliferation and osteogenic differentiation, we have been developing bone graft biomaterials containing poly(P) for accelerated bone regeneration. In our previous studies, we demonstrated that blocks of interconnected porous calcium hydroxyapatite (IP-CHA) 11) with adsorbed poly(P) accelerated bone formation 12,13) . However, blocks of graft material are difficult to apply in clinical GBR because the topology of the dental bone defect caused by surgical resection and subsequent inflammation is complex and not predictable before treatment.…”

Section: Introductionmentioning

confidence: 99%

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Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

et al. 2014

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Inorganic polyphosphate (poly(P)) is recognized as a therapeutic agent that promotes fibroblast growth factor and enhances osteogenic differentiation, and in vivo, when adsorbed onto interconnected porous calcium hydroxyapatite (IP-CHA) enhances bone regeneration. The present study focused on the effect of poly(P) adsorbed onto IP-CHA granules (Poly(P)/IP-CHA) in guided bone regeneration (GBR). Dental implants were placed into the edentulous mandibular areas of five Beagle-Labrador hybrid dogs with screw expose on the buccal side, and then bone defects were filled Poly(P)/IP-CHA (test) or IP-CHA (control). After 12 weeks, histological evaluation and histomorphometrical analysis were performed. Newly-bone formation around exposed implant screw was clearly detected in the test-group. The ratio for regenerated bone height in the test group versus the control-group was 85.6±20.2 and 62.6±23.8, respectively, with no significant difference, while, that for bone implant contact was significantly higher (67.9±11.8 and 48.8±14.1, respectively). These findings indicate that Poly(P)/IP-CHA enhances bone regeneration in GBR.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

et al. 2014

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“…[2][3][4][5][6][7][8][9][10][11][12] A very successful strategy was to enhance bone regeneration by adsorbing inorganic polyP onto HAp. [13][14][15][16][17][18] PolyP, which is an orthophosphate polymer found in mammalian organisms, 19 stabilizes basic cell growth and differentiation promoting bone regeneration. [20][21][22] Furthermore, short chains of polyP have been detected, together with  4 7…”

Section: Introductionmentioning

confidence: 99%

An experimental-computer modeling study of inorganic phosphates surface adsorption on hydroxyapatite particles

et al. 2015

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The adsorption of orthophosphate, pyrophosphate, triphosphate and a trisphosphonate onto hydroxyapatite has been examined using experiments and quantum mechanical calculations. Adsorption studies with FTIR and X-ray photoelectron spectroscopies have been performed considering both crystalline hydroxyapatite (HAp) and amorphous calcium phosphate particles, which were specifically prepared and characterized for this purpose. Density Functional Theory (DFT) calculations have been carried out considering the (100) and (001)

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Hydroxyapatite with Permanent Electrical Polarization: Preparation, Characterization, and Response against Inorganic Adsorbates

et al. 2018

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Permanently polarized hydroxyapatite (HAp) particles have been prepared by applying a constant DC of 500 V at 1000 °C for 1 h to the sintered mineral. This process causes important chemical changes, as the formation of OH defects (vacancies), the disappearance of hydrogenophosphate ions at the mineral surface layer, and structural variations reflected by the increment of the crystallinity. As a consequence, the electrochemical properties and electrical conductivity of the polarized mineral increase noticeably compared with as-prepared and sintered samples. Moreover, these increments remain practically unaltered after several months. In addition, permanent polarization favours significantly the ability of HAp to adsorb inorganic bioadsorbates in comparison with as-prepared and sintered samples. The adsorbates cause a significant increment of the electrochemical stability and electrical conductivity with respect to bare polarized HAp, which may have many implications for biomedical applications of permanently polarized HAp.

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Effect of combined application of bFGF and inorganic polyphosphate on bioactivities of osteoblasts and initial bone regeneration

Cited by 37 publications

References 35 publications

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

An experimental-computer modeling study of inorganic phosphates surface adsorption on hydroxyapatite particles

Hydroxyapatite with Permanent Electrical Polarization: Preparation, Characterization, and Response against Inorganic Adsorbates

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