Effect of Combination of Follicle Size, FSH and Cysteamine on In Vitro Production of Sheep Embryos

Abstract

The participatory relationship among the follicle size, follicle stimulating hormone (FSH), and cysteamine (antioxidant agent) contribute to the production of embryos characterized by abundance and good quality. The aim of this study was to evaluate the efficacy of FSH, cysteamine and follicle size on <em>in vitro</em> embryo production of Awassi sheep oocytes. Follicles sizes were determined into two groups: small follicles (1-2 mm) and large follicles (> 2 mm). Oocytes were matured across two increasingly shared levels of FSH and cysteamine: A (40 ng/ml + 50 μM) and B (60 ng/ml + 100 μM). Results of the bilateral interaction showed significant differences across the follicle size (large follicles group) and the maturation treatment (B medium) in the rates of fertilization (highest value: 67.51%; p= 0.02), cleavage (highest value: 65.41%; p= 0.01), 2-16 cell stage (lowest value: 2.29%; p= 0.0001), blastocyst stage (highest value: 44.82%; p= 0.04), down to morula stage arrest (lowest value: 55.17%; p= 0.04) and Type I embryos (highest value: 52.87%; p= 0.03). Likewise, matured oocytes of small follicles group (B medium) attained the highest rate of morula stage (56.60%; p= 0.03). No significant differences were observed in Type II and Type III embryos. In order to obtain high yields of good quality embryos, it is advised to add FSH and cysteamine with levels of 60 ng/ml and 100 μM respectively to maturation medium of ovine oocytes obtained from follicles with a diameter > 2 mm.

Effectiveness of the Ovarian Scraping Method in In Vitro Maturation of Equine Oocytes During the Breeding and Non-Breeding Seasons

“…Normally, this activity is manifested by a series of changes in the pathways and timing of action of the hormone related to the maturation process. Not only that, in the dialectic of the dynamic follicular wave, the group of follicles in the ovary will be subjected to a series of hormonal activities (LH surge) that will cause the emergence of the dominant follicle that includes an oocyte qualified to complete the processes of maturatin and subsequent nuclear division in preparation for a successful fertilization (Ginther et al, 1996;Aryan et al, 2016;Mardenli et al, 2020;Mardenli et al, 2021) The previous scenario described is the reason for the emergence of different degrees of developmental competence of the oocytes, which in turn determines whether the oocyte is cultivable or not (Mihm et al, 2000). In literature, there were many methods of oocyte collection (e.g., slicing, aspiration and ovarian scraping), which led to obtaining highly dispersed rates due to the type of oocytes targeted (mature or immature) according to each method.…”

Evaluation of the In Vitro Embryo Production of Farm Animals Under the Circumstances of Embryo Transfer Technology

“…In this regard, more research has been conducted by relying on innovative technologies and methods, the most important of which is the technique of in vitro embryo production (IVEP). It can be said that the IVEP technology has achieved a significant harvest in the yield of the produced embryos despite the difficulties encountered and the relatively low productivity levels of these embryos [5][6][7][8]. In the current period, the integrated protocols in which many additives and improved culture media were used have eventually led to noticeable differences in the outcome of embryos produced.…”

Role of Some Inducing Additives in in vitro Maturation Environment of Farm Animal's Oocytes: Status Evaluation

“…Thus, this made maturation processes stabilize in a new phase completely different from the initial stage that prevailed in a relatively long period. Hormones are one of the most important additives in IVM, especially the gonadotropic hormones (FSH, LH and CG), as the supply of these additives during the period of maturation (24-27 hours) has led to a significant increase in the yield of embryos (7,8,9,10,11,12). There has been a widespread trend in studies towards the embodiment of serum and FF in the IVM environment since they achieve strong integration of performance with common maturation media.…”

“…In its action, cysteamine resembles the action of antioxidant enzymes such as superoxide dismutase which eliminates oxygen (O 2 ). The IVM environment lacks antioxidants, and as a consequence, cysteamine had to be added to support the antioxidant resistance caused by reactive oxygen species (ROS) (9,13). In this review, the vital role and the actual reality of the inducing additives (hormones, FCS, FF and cysteamine) in the IVEP technology were highlighted in terms of usage, concentrations, advantages, and limitations.…”