Effect of collagen solution application on healing following surgical treatment in colony‐bred monkeys

Abstract

The present study was performed to investigate the effects on periodontal regeneration in flap surgery of acid conditioning and the application of a collagen solution to the root surface. Bone defects were artificially produced in 4 monkeys by removing cortical bone in an apical direction within 4 mm from the alveolar bone crest, following incision and elevation of the buccal gingiva of the second premolars, first molars and lateral incisors of the maxilla and mandibula. After root planing, the root surface at the bottom of the bone defect was notched. The root surfaces were then subjected to various types of root preparation and divided into (1) a group given topical application of collagen solution following acid conditioning, (2) a group given topically applied collagen solution, (3) a group given acid conditioning and (4) controls. The animals were sacrificed 3 or 6 weeks after surgery and histopathologically examined. The results indicated that the method of applying collagen solution to the root surface after acid conditioning in flap surgery promoted suppression of epithelial migration and new cementum formation more effectively than the conventional method of preparation using acid conditioning alone.

Effect of cyclic freeze–thawing process on the structure and properties of collagen

“…Collagen is usually used in the form of solution prepared by the dissolution of collagen in acid solution, for instance, collagen solution applied on healing surgical treatment and as cosmetic material [3,4]. However, the thermal stability of collagen solution is relatively low (∼40 • C), even if temperature was far lower than 40 • C, denaturation would occur and thus the native triple-helical structure would be destroyed [5,6].…”

Tetracycline‐Coated Polytetrafluoroethylene Barrier Membranes in the Treatment of Intraosseous Periodontal Lesions

“…The antiproteolytic properties of tetracyclines may help reduce alveolar bone resorption and periodontal disease progression. 22 Topical tetracyclines have been applied in acrylic strips, 25,26 resorbable cellulose preparations, 27 crosslinked collagen films, 28 and biodegradable polylactide/ polycolide implants. 29 In ePTFE membrane-assisted therapy, Parashis and Mitsis 30 studied the effect of tetracycline root conditioning in guided tissue regeneration with e-PTFE barrier membranes and detected no clinical benefit of tetracycline compared to controls, probably because the obtained tetracycline concentration was too low to effectively control the barrier membrane microbiota.…”