Effect of Colchicine on the Induction of Prunella vulgaris for. albiflora Nakai

Abstract

This study was conducted to find out the effective induction method of tetraploid plants to obtain potential data for cultivating superior varieties by colchicine treatment. The seed germination were decreased by the higher concentration of colchicine treatment and longer soaking time. A total of 907 individuals were germinated in 16 treated plots except control (untreated plot) and 28 tetraploids were induced which was about 3.1% of the number of seed germinated. The plant regeneration rate by colchicine treatment on explant of Prunella vulgaris for. albiflora Nakai under in vitro culture was decreased with the higher concentration of colchicine. While a total of 312 individuals were regenerated in all treatments, the explant was soaked in more than 0.05% for over 1 hour, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 6 hours and 12 hours, 37 tetraploids were induced, which was about 57.8% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploid, the peak DNA content of G1 phase was 101.3 for diploid and 197.2 for tetraploid. The result confirmed the doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 19.3 in tetraploid, which was around 1.9 times as much as diploid.

A comparative study of morphological characteristics in diploid and tetraploid (auto and allotetraploids) Citrullus genotypes

“…In parallel with our results, it was reported that the average number of stomata per unit area in the leaves of diploid plants is higher than the number of stomata per unit area of leaves of polyploid plants (Chen, 2010;Bae et al, 2020). It was also reported that the stomatal length and width increased in polyploid plants after colchicine application (Yang et al, 2006;Kwon et al, 2015).…”

“…A decrease in the survival rate after colchicine treatment has been reported in many species. The difference between reported results can be explained by the genotypic difference, colchicine concentration and duration of application, and the conditions of application (in vivo/in vitro) (Do Valle and Penteado, 2000;Kerdsuwan and Te-chato, 2012;Kwon et al, 2015).…”

Paclitaxel and Caffeine–Taurine, New Colchicine Alternatives for Chromosomes Doubling in Maize Haploid Breeding

“…Severe effects of different colchicine concentrations were also reported in Phlox drummondi [63]. In Prunella vulgaris and Dendrocalumus brandisii, a higher colchicine concentration with processing times decreased the germination of seeds [64,65]. Colchicine-treated okra seeds' germination was calculated 79.3%, which is significantly lower than untreated plants at 94.8% [66].…”

Assessment of Biological Damage and Toxic Potency of Colchicine in Gladiolus (Gladiolus Grandiflorus) Plants

“…It has a complex mechanism of action and multiple properties (Eng et al, 2021). It works by inhibiting the spindle fiber formation by combining tubulins during somatic cell division and inducing polyploidy by interfering with microtubule formation and chromosome anadol movement in the middle of the cell division process (Kwon et al, 2014). It also induces polyploidy by preventing chromosome segregation during meiosis (Ade and Rai, 2010).…”