Effect of coconut (Cocos nucifera L.) water extracts on adventitious root development in vegetative propagation of Dracaena purplecompacta L.

Abstract

Propagation by softwood canes and cuttings is preferred as a practical system for vegetative reproduction of many ornamental plant species, despite the advances in tissue culture techniques. Dracaena purplecompacta L. is a species that has a high demand for exports. Conversely, coconut water (CW) is a rich supplement that naturally contains plant growth regulators such as indole acetic acid (IAA). The objective of this work was to evaluate the potential of CW extracts containing natural IAA, on adventitious root development in vegetative propagation of ornamental plant canes of D. purplecompacta L. Five different concentrations (28, 57, 143, 286, 571 lM of natural IAA) of CW extracts were tested. Another set of treatment was carried out with the same concentrations of authentic IAA hormone for comparison purpose. The 143-lM IAA CW extract recorded the best root induction and development. It was found that the root expression was faster (5 weeks) with the use of the novel method. In the conventional method, the canes are propagated by quick dip application of commercial product containing artificial hormone IAA and placing them on coir fiber dust beds. It takes up to 6 weeks for the canes to develop adventitious roots to the desired level. Steeping canes in 143-lM IAA CW extract improved rooting in D. purplecompacta L., and it was comparable to the application of 143-lM authentic IAA. The study indicates that adventitious root development, shoot development, and leaf emergence of D. purplecompacta L. is promoted by IAA CW extracts.

The Effects of Different Media, Sucrose Concentrations and Natural Additives on Plantlet Growth of Phalaenopsis Hybrid 'Pink'

“…Coconut water contains many nutritional and/or hormonal substances including diphenyl urea, which acts as cytokinin and this growth regulator induces the growth and cell division [19,44] . Others also reported an effective growth and regeneration of plants in CW supplemented medium and this is due to the presence of natural contents of cytokinin and auxin [45] . In the present study, the concentrations of 10 and 20 g L -1 CW supplemented with either half-strength MS medium or VW medium reduced the production of rooting, leaf, fresh weight, dry weight, plant height and survival frequency of Phalaenopsis hybrid 'Pink' shoots after four months of culture in all sucrose concentrations.…”

Seedling Propagation of Kulo Chrysanthemum by Tissue Culture

“…Media added coconut water and IAA resulted in higher root length and root number compared to treatment added BAP. Agampodi and Jayawardena (2008) recorded treatment of coconut water extract contains 143 uM IAA resulting the best root induction and development.…”

Analysis of Trace Elements During Different Developmental Stages of Somatic Embryogenesis in Plantago ovata Forssk Using Energy Dispersive X-ray Fluorescence

“…Another additive, coconut water is a rich supplement that naturally contains diphenyl-urea, a chemical with cytokinin-like activity [29] that supports the growth of somatic embryogenesis. Addition of coconut water in the media help for development of plant propagated in vitro [14,30,31].…”

“…Coconut water contains plant growth regulators and is liberally made use in tissue culture. Earlier researchers have investigated the addition of coconut water in media and observed that CW enhanced the development of embryos in vitro [14,30,31,36].…”