Abstract:Controlled activation of lymphangiogenesis through functional biomaterials represents a promising approach to support wound healing after surgical procedures, yet remains a challenge. In a synthetic biological approach, we therefore set out to mimic the basal microenvironment of human primary dermal lymphatic endothelial cells (LECs) during lymphangiogenesis. As the extracellular matrix component hyaluronan (HA) regulates lymphangiogenesis, we designed a bifunctional surface in which adhesive peptide ligands a… Show more
“…The effect of HA length on culture of endothelial cells has been investigated in several studies, 51,52 but mainly using HA in solution (added to the culture medium). Covalent immobilization of HA on solid surfaces 53,54 has also been investigated, but the methods used require chemical modification of HA. Using the Pep-1 SAM described in the previous sections, we have studied the effect of HA molecular weight on HUVECs, where HA is presented at surfaces in its native form without covalent immobization.…”
Section: Cell Culturementioning
confidence: 99%
“…These results were consistent with the literature reporting that low molecular weight HA can stimulate cell motility while high molecular weight HA inhibits. 53 In the cell viability assay, cells seeded on bare Au were used as control cells, and the percentage difference between treated (seeded on peptide SAMs with and without HA) and control cells was calculated (Fig. 5C).…”
“…The effect of HA length on culture of endothelial cells has been investigated in several studies, 51,52 but mainly using HA in solution (added to the culture medium). Covalent immobilization of HA on solid surfaces 53,54 has also been investigated, but the methods used require chemical modification of HA. Using the Pep-1 SAM described in the previous sections, we have studied the effect of HA molecular weight on HUVECs, where HA is presented at surfaces in its native form without covalent immobization.…”
Section: Cell Culturementioning
confidence: 99%
“…These results were consistent with the literature reporting that low molecular weight HA can stimulate cell motility while high molecular weight HA inhibits. 53 In the cell viability assay, cells seeded on bare Au were used as control cells, and the percentage difference between treated (seeded on peptide SAMs with and without HA) and control cells was calculated (Fig. 5C).…”
“…Indeed, it was shown in several studies that LMW HA induces lymphangiogenesis through LYVE-1 mediated signaling pathways. [46][47][48] One recent study also shows that HA-binding peptide modulates EC spreading and migration through focal adhesion kinase (FAK). 49 Despite the unique expression of HA receptors by LECs, the effect of HA on the regulation of lymphatic characteristic markers has not been explored yet.…”
Lymphatic endothelial cells (LECs) play a critical role in the formation and maintenance of the lymphatic vasculature, which is essential for the immune system, fluid balance, and tissue repair. However,...
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