Synthetic hyaluronic acid coating preserves the phenotypes of lymphatic endothelial cells

Abstract

Lymphatic endothelial cells (LECs) play a critical role in the formation and maintenance of the lymphatic vasculature, which is essential for the immune system, fluid balance, and tissue repair. However,...

Hyaluronic Acid Hydrogels with Phototunable Supramolecular Cross-Linking for Spatially Controlled Lymphatic Tube Formation

“…We demonstrate that these dynamic hydrogels can provide spatially controlled substrates for the culture of human LECs to direct lymphatic CLS formation. The presentation of HA to the surface of LECs has been shown to preserve the lymphatic phenotypes . Modulating the viscoelastic properties of the HA hydrogels can further promote lymphatic CLS formation by enhanced expression of key lymphatic markers, such as LYVE-1 , podoplanin , and Prox1 , along with expression of Nrp2 , VEGFR2 , and VEGFR3 to enhance VEGF-C stimulation.…”

“…The presentation of HA to the surface of LECs has been shown to preserve the lymphatic phenotypes. 71 Modulating the viscoelastic properties of the HA hydrogels can further promote lymphatic CLS formation by enhanced expression of key lymphatic markers, such as LYVE-1, podoplanin, and Prox1, along with expression of Nrp2, VEGFR2, and VEGFR3 to enhance VEGF-C stimulation. Whereas previous studies have shown the importance of matrix stiffness for lymphatic tube formation, 46,54 this report offers evidence supporting a role for matrix viscoelasticity in promoting lymphatic development and lymphatic CLS formation.…”

Triangular silver nanoprism-based chemosensor for recognition of hyaluronic acid in human biofluids: a new platform for monitoring osteoarthritis treatment using smartphone-assisted digital image analysis

“…Furthermore, the lymphatic system aids in fluid drainage, removing immune complexes and interstitial matrix components that break down during regular body balance and tissue damage. 1…”