Effect of clofibrate and hydrocortisone on intestinal trichinellosis in mice

Durán, M. L. Sanmartín; Santamariña, Marta; Ubeira, F. M.

doi:10.1016/0304-4017(86)90144-5

Cited by 14 publications

(3 citation statements)

References 7 publications

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“…This strain is that coded as MFEL/ES/S2 GM-1-ISS48 (T1a) by La and Pozio et al (1992). It was maintained in CD-1 mice, and larvae were obtained by HCl/pepsin digestion as described previously (Ubeira et al, 1987), except that digestion time was reduced to 4 h. Adult worms were counted by direct observation under a dissection microscope as described elsewhere (Sanmartín et al, 1986), some drops of iodine being added to improve visualization. The larvae were administered to mice orally in 0.4 ml of saline (300 larvae/mouse) through an 18-gauge feeding needle attached to an automatic Cornwall-type syringe.…”

Section: Parasites and Infectionsmentioning

confidence: 99%

Anthelminthic and antiallergic activities of Mangifera indica L. stem bark components Vimang and mangiferin

Garcı́a¹,

Escalante

Delgado

et al. 2003

Phytotherapy Research

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This study investigated the antiallergic and anthelmintic properties of Vimang (an aqueous extract of Mangifera indica family stem bark) and mangiferin (the major polyphenol present in Vimang) administered orally to mice experimentally infected with the nematode, Trichinella spiralis. Treatment with Vimang or mangiferin (500 or 50 mg per kg body weight per day, respectively) throughout the parasite life cycle led to a significant decline in the number of parasite larvae encysted in the musculature; however, neither treatment was effective against adults in the gut. Treatment with Vimang or mangiferin likewise led to a significant decline in serum levels of specific anti-Trichinella IgE, throughout the parasite life cycle. Finally, oral treatment of rats with Vimang or mangiferin, daily for 50 days, inhibited mast cell degranulation as evaluated by the passive cutaneous anaphylaxis test (sensitization with infected mouse serum with a high IgE titre, then stimulation with the cytosolic fraction of T. spiralis muscle larvae). Since IgE plays a key role in the pathogenesis of allergic diseases, these results suggest that Vimang and mangiferin may be useful in the treatment of diseases of this type.

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Section: Parasites and Infectionsmentioning

confidence: 99%

Anthelminthic and antiallergic activities of Mangifera indica L. stem bark components Vimang and mangiferin

Garcı́a¹,

Escalante

Delgado

et al. 2003

Phytotherapy Research

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show abstract

“…Larvae obtained from infected CD-I~ mice by digestion in HCl-pepsin [24] were washed with saline and administered to experimental mice by orogastric tube. The infective dose in each case was 300 viable larvae per mouse.…”

Section: Methodsmentioning

confidence: 99%

The antiphosphorylcholine plaque-forming cell responses induced by the nematode Trichinella in BWF1 mice

Ubeira

Leiro

Prado

et al. 1987

Med Microbiol Immunol

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We have tested the ability of the nematode Trichinella to infect young and old (NZB x NZW) F1 (BWF1) mice. We report the capacity of these mice to respond to the parasite antigens containing the epitope phosphorylcholine. The values obtained in adult worm intestinal retention rates and in the number of encysted larvae on the 35th day postinfection showed that the old BWF1 mice are more susceptible than young BWF1 and control (BALB/c x CBA/j)F1 (BCF1) mice to Trichinella infection. However, unlike the BCF1 mice, young BWF1 mice were unable to produce a good anti-phosphorylcholine plaque-forming cell response after the killing of Trichinella larvae by the anthelminthic mebendazole. Old BWF1 mice presented a discrete response which is discussed. Finally, our results seem to indicate that the lack of anti-phosphorylcholine response in young BWF1 mice after mebendazole treatment may be related to the high susceptibility of these mice to the suppressive properties of encysted Trichinella larvae against their own antigens.

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“…Trichinella nelsoni (listed as C-76) isolated in Galicia (N.W. Spain) in 1976 (Sanmartin-Duran, Santamarina & Ubeira, 1986). Trichinella nativa was a gift from Dr Boev of the Helminthological Institute, Alma-Ata, Kazakhstan, USSR, and Trichinella pseudospiralis was obtained from the Institute de Parasitologia Lopez Neyra, Granada, Spain.…”

Section: Parasitesmentioning

confidence: 99%

Immune response toTrichinellaepitopes: the antiphosphorylcholine plaque-forming cell response during the biological cycle

et al. 1987

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Phosphorylcholine (PC), an immunodominant component of the cell wall of certain bacteria, fungi and nematodes, is known to induce low anti-PC antibody levels during natural infection by Trichinella spiralis. This article reports a study in which spleen cells from BCF1 mice infected with Trichinella sp. larvae were found to produce large numbers of direct haemolytic plaques in response to PC conjugated to sheep red blood cells (SRBC) after muscle-encysted larvae had been killed by treatment with mebendazole. Inhibition of the response by PC-chloride, immunodiffusion and immunoelectrophoretic studies with the anti-PC IgA (TEPC-15) and anti-idiotype T15 serum assays showed the plaque-forming cell (PFC) response to be specific for PC. The absence of haemolytic plaques when unconjugated SRBC or TNP-SRBC were used as indicator cells ruled out involvement of a polyclonal response. Greatest anti-PC PFC response was found to be associated with a microsomal fraction designated FCp1, a particulate fraction behaving as a thymus-dependent antigen. The FCp1 fractions from all four strains of Trichinella employed induced anti-PC PFC responses when injected into mice. These results suggest that FCp1 is a suitable antigen for use in detailed studies of immune responses to Trichinella and related parasites.

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Effect of clofibrate and hydrocortisone on intestinal trichinellosis in mice

Cited by 14 publications

References 7 publications

Anthelminthic and antiallergic activities of Mangifera indica L. stem bark components Vimang and mangiferin

Anthelminthic and antiallergic activities of Mangifera indica L. stem bark components Vimang and mangiferin

The antiphosphorylcholine plaque-forming cell responses induced by the nematode Trichinella in BWF1 mice

Immune response toTrichinellaepitopes: the antiphosphorylcholine plaque-forming cell response during the biological cycle

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