Effect of Cholinergic Blockade on Glucocorticoid Regulation of NPY and Catecholamines in the Rat Adrenal Gland

Laborie, Christine; Bernet, François; Dutriez-Casteloot, Isabelle; Lésage, Jean; Dupouy, Jean‐Paul

doi:10.1159/000127225

Cited by 10 publications

(2 citation statements)

References 28 publications

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“…The time course and dose range were based on previous data (Nair and Bonneau, 2006; O'Callaghan et al , 2005). Importantly, metyrapone has been shown to effectively deplete circulating plasma corticosterone levels under basal (Laborie et al , 1995, 1997) and stressed (Krugers et al , 2000) conditions. The glucocorticoid receptor antagonist mifepristone (also called RU38486; Sigma-Aldrich) was suspended in 0.5% CMC and dissolved by sonicating for 15 min, and was delivered i.p.…”

Section: Methodsmentioning

confidence: 99%

CRF-1 Antagonist and CRF-2 Agonist Decrease Binge-Like Ethanol Drinking in C57BL/6J Mice Independent of the HPA Axis

Lowery

Spanos

Navarro

et al. 2010

Neuropsychopharmacol

119

131

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Recent evidence suggests that corticotropin-releasing factor (CRF) receptor (CRFR) signaling is involved in modulating binge-like ethanol consumption in C57BL/6J mice. In this report, a series of experiments were performed to further characterize the role of CRFR signaling in binge-like ethanol consumption. The role of central CRFR signaling was assessed with intracerebroventricular (i.c.v.) infusion of the nonselective CRFR antagonist, a-helical CRF 9-41 (0, 1, 5, 10 mg/1 ml). The contribution of central CRF type 2 receptor (CRF 2 R) signaling was assessed with i.c.v. infusion of the selective CRF 2 R agonist, urocortin (Ucn) 3 (0, 0.05, 0.1, or 0.5 mg/1 ml). The role of the hypothalamic-pituitary-adrenal (HPA) axis was assessed by pretreating mice with intraperitoneal (i.p.) injection of (1) the corticosterone synthesis inhibitor, metyrapone (0, 50, 100, 150 mg/kg) or (2) the glucocorticoid receptor antagonist, mifepristone (0, 25, 50 mg/kg), and (3) by using radioimmunoassay to determine whether binge-like ethanol intake influenced plasma corticosterone levels. Finally, we determined whether the ability of the CRF 1 R antagonist, CP-154,526 (CP; 0, 10, 15 mg/kg, i.p.), to blunt binge-like drinking required normal HPA axis signaling by comparing the effectiveness of CP in adrenalectomized (ADX) and normal mice. Results showed that i.c.v. infusion of a 1 mg dose of a-helical CRF 9-41 significantly attenuated binge-like ethanol consumption relative to vehicle treatment, and i.c.v. infusion of Ucn 3 dose-dependently blunted binge-like drinking. On the other hand, metyrapone nonselectively reduced both ethanol and sucrose consumption, mifepristone did not alter ethanol drinking, and binge-like drinking did not correlate with plasma corticosterone levels. Finally, i.p. injection of CP significantly attenuated binge-like ethanol intake in both ADX and normal mice. Together, these results suggest that binge-like ethanol intake in C57BL/6J mice is modulated by CRF 1 R and CRF 2 R signaling, such that blockade of CRF 1 R or activation of CRF 2 R effectively reduces excessive ethanol intake. Furthermore, normal HPA axis signaling is not necessary to achieve binge-like drinking behavior.

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Section: Methodsmentioning

confidence: 99%

CRF-1 Antagonist and CRF-2 Agonist Decrease Binge-Like Ethanol Drinking in C57BL/6J Mice Independent of the HPA Axis

Lowery

Spanos

Navarro

et al. 2010

Neuropsychopharmacol

119

131

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“…The percentage recovery of a known amount of this steroid was over 95%. Corticosterone levels were determined by RIA using a highly specific corticosterone antiserum according to a previously detailed procedure (20) with a detection threshold of Figure 5 Plasma ACTH (A) and corticosterone (B) concentrations in 2-week-old newborn rats (W2) before (0 min) and 30 min after injection of 0.9% NaCl, morphine (20 mg/kg), NAL (10 mg/kg), L-NAME (100 mg/kg), morphine and NAL, or morphine and L-NAME. Values are means^S.E.M.…”

Section: Radioimmunoassaysmentioning

confidence: 99%

Morphine-induced stimulation of pituitary-adrenocortical activity is mediated by activation of nitric oxide in the early stages of postnatal life in the rat

Lésage

Bernet²,

Montel³

et al. 2001

European Journal of Endocrinology

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Objective: The first aim of the present study was to determine if morphine, a prototypic m-opioid agonist drug, affects pituitary±adrenocortical activity in developing rat pups (first and second weeks of postnatal life). The second aim of this study was to explore, in vivo, if nitric oxide (NO) could be involved in the neurohormonal response to morphine in the early stages of postnatal life. Methods: Plasma ACTH and corticosterone concentrations were determined by RIA in rat pups n 5±14 rats/experimental group) after they had been killed by decapitation. In a first experiment, 1-day and 1-and 2-week-old rats were treated s.c. with morphine (20 mg/kg) or with vehicle (0.9% NaCl) and killed 5±90 min later. In a second experiment, 2-week-old pups were pretreated s.c. with naltrexone (NAL; 0.4 mg/kg or 10 mg/kg), and injected 1 h later with either morphine (20 mg/kg) or vehicle, and killed 30 min later. Some pups injected with only NAL were killed 60 or 90 min later. On the other hand, pups injected with NAL (10 mg/kg) or NAL and morphine were killed 30 min later. In a third experiment, 2-week-old pups were pretreated s.c. with N-v-nitro L arginine methylester (L-NAME; 30 mg/kg or 100 mg/kg), and injected 1 h later with either morphine (20 mg/kg) or vehicle, and killed 30 min later. Moreover, some pups injected with L-NAME (100 mg/kg) or L-NAME with morphine were killed 30 min later. In a final experiment, pups were injected s.c. with either S-nitroso-N-acetylpenicillamine (SNAP; 5 mg/kg) or vehicle, and killed 60 or 90 min later. Results: Morphine administered to rat pups elicited marked rises in both ACTH and corticosterone secretion. Moreover, these responses increased with advancing postnatal age. In 2-week-old rat pups, NAL, a competitive antagonist at m-opioid receptors, administered alone increased both plasma ACTH and corticosterone concentrations 30 min later. L-NAME, a specific NO synthase inhibitor, did not affect plasma ACTH and corticosterone concentrations 30 min later when administered alone. NAL, when concomitantly administered with morphine, was unable to block morphine responses. In contrast, morphine responses were blocked by pretreatment (60 min before) with NAL or with L-NAME. Acute injection of SNAP increased both ACTH and corticosterone release. Conclusion: Our results suggest that opioids have controversial effects on pituitary±adrenocortical activity in the early postnatal period in the rat, and that endogenous NO is one of the major factors in the response of the pituitary±adrenocortical axis to morphine.

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Diazepam-like effects of a fish protein hydrolysate (Gabolysat PC60) on stress responsiveness of the rat pituitary-adrenal system and sympathoadrenal activity

et al. 2000

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Effect of Cholinergic Blockade on Glucocorticoid Regulation of NPY and Catecholamines in the Rat Adrenal Gland

Cited by 10 publications

References 28 publications

CRF-1 Antagonist and CRF-2 Agonist Decrease Binge-Like Ethanol Drinking in C57BL/6J Mice Independent of the HPA Axis

CRF-1 Antagonist and CRF-2 Agonist Decrease Binge-Like Ethanol Drinking in C57BL/6J Mice Independent of the HPA Axis

Morphine-induced stimulation of pituitary-adrenocortical activity is mediated by activation of nitric oxide in the early stages of postnatal life in the rat

Diazepam-like effects of a fish protein hydrolysate (Gabolysat PC60) on stress responsiveness of the rat pituitary-adrenal system and sympathoadrenal activity

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