Effect of cholesterol loaded cyclodextrin supplementation on tyrosine phosphorylation and apoptosis like changes in frozen thawed Hariana bull spermatozoa

Yadav, Hanuman Prasad; Kumar, Anuj; Shah, Nimesh; Chauhan, Dharmendra Singh; Saxena, Atul; Yadav, Sarita; Swain, Dilip Kumar

doi:10.1016/j.theriogenology.2017.04.016

Cited by 33 publications

(32 citation statements)

References 35 publications

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“…An indirect immunofluorescence assay was used for the localization of phosphotyrosine containing protein in frozen‐thawed sperm as described by Tardif, Dube, Chevalier, and Bailey (). The detailed procedure for slide preparation and immunolocalization of tyrosine‐phosphorylated proteins has been described elsewhere (Yadav et al, ). A total of 200 sperm per slide were examined, and different localization patterns were classified according to Cormier and Bailey (): pattern A, that is, uniform fluorescence over the entire acrosome (low capacitation level); pattern E, that is, a short line or triangle of fluorescence in the equatorial segment (medium capacitation level); and pattern EA, that is, fluorescence at both equatorial and anterior acrosomal regions (high capacitation level).…”

Section: Methodsmentioning

confidence: 99%

Effect of various levels of dissolved oxygen on reactive oxygen species and cryocapacitation‐like changes in bull sperm

Amin

Prasad

Ghosh

et al. 2018

Reprod Domestic Animals

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The present investigation was carried out to study the effect of various levels of dissolved oxygen (DO) on reactive oxygen species (ROS) and cryocapacitation-like changes in bull sperm. Egg yolk-Tris-glycerol (EYTG) extender was split into four subextenders; viz., Extender I (control; no flushing with liquid nitrogen (LN )), Extender II, Extender III and Extender IV were flushed with LN for 40, 16 and 8 min, respectively. The DO levels were standardized to 11.7, 2, 4 and 8 ppm, respectively, in control (Extender I), Extender II, Extender III and Extender IV. Ejaculates with mass motility of ≥ 3+ were divided into group I (diluted with Extender I), group II (diluted with Extender II), group III (diluted with Extender III) and group IV (diluted with Extender IV) up to 80 × 10 sperm/ml. Extended semen samples were packed in French mini straws (0.25 ml), equilibrated and cryopreserved. Semen samples were evaluated at prefreeze and post-thaw stage for various parameters (DO, progressive motility (PM), viability (VIB), acrosomal integrity (AI), hypo-osmotic swelling (HOS) test, ROS, cholesterol (C) and phospholipid (P). The percentage of PM, VIB, AI, HOS test, cholesterol (C) and phospholipid (P) levels, and capacitated sperm were significantly (p < 0.05) higher in groups III and IV as compared to groups I and II. However, the acrosome-reacted sperm (%; pattern AR) were significantly (p < 0.05) decreased in group III as compared to all other groups. Besides the proportion of sperm displaying tyrosine-phosphorylated pattern, EA (fluorescence at both equatorial and anterior acrosomal regions, i.e. high capacitation level) was significantly (p < 0.05) reduced in group III compared to all other groups. In conclusion, varying DO levels in the extender significantly affect sperm quality, ROS production and capacitation-like changes in bulls.

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Section: Methodsmentioning

confidence: 99%

Effect of various levels of dissolved oxygen on reactive oxygen species and cryocapacitation‐like changes in bull sperm

Amin

Prasad

Ghosh

et al. 2018

Reprod Domestic Animals

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“…The conventional freezing technique is still most widely used, and the associated cryo‐injury can be minimised by altering the type of freezing media, cryoprotectants, antioxidants, protein source, and reducing the mechanical and oxidative stresses involved in the semen processing, storage and transfer. In this context, various concentrations of cholesterol‐loaded cyclodextrins (CLC) have been used to prevent cold shock and to enhance cryosurvivability of spermatozoa in cattle (Purdy & Graham, 2004; Yadav et al., 2017) and buffalo bulls (Ejaz et al., 2016; Rajoriya et al., 2016). The addition of CLC at the rate of 3 mg/120 × 10 6 spermatozoa in bubaline species protects sperm progressive motility, membrane fluidity, and enhances in vitro fertility rate (40% more zona binding and 35% higher cleavage rate) (Rajoriya et al., 2020).…”

Section: Aspects and Prospects To Counter Cryodamagementioning

confidence: 99%

Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview

et al. 2021

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Sperm cryopreservation is an important adjunct to assisted reproduction techniques (ART) for improving the reproductive efficiency of dairy cattle and buffaloes. Improved understanding of mechanisms and challenges of bovine semen cryopreservation is vital for artificial insemination on a commercial basis. Although cryopreservation of bovine spermatozoa is widely practiced and advanced beyond that of other species, there are still major gaps in the knowledge and technology. Upon cryopreservation, disruption of spermatozoal plasma membrane configuration due to alterations in metabolic pathways, enzymes and antioxidants activity add to lower efficiency with loss of sperm longevity and fertilising ability. Therefore, the effective amalgamation of cryo‐variables like ambient temperature, cooling and thawing rates, nucleation temperature, type and concentration of the cryoprotectant, seminal plasma composition, free radicals and antioxidant status are required to optimise cryopreservation. Novel strategies like supplementation of cholesterol‐loaded cyclodextrins (CLC), nanovesicles, osteopontin, antioxidants, etc., in an extender and recent techniques like nano‐purification and modified packaging have to be optimised to ameliorate the cryodamage. This article is intended to describe the basic facts about the sperm cryopreservation process in bovine and the associated biochemical, biophysical, ultra‐structural, molecular and functional alterations.

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“…Spermatozoal phospholipid configuration and mitochondrial membrane fluidity are highly vulnerable to Time Dependent Impact of Reactive Oxidants on Seminal Attributes of Murrah Bull during Cryopreservation and Storage these oxidative attacks since the extended semen lack significant antioxidant protection and appropriate armory of defensive enzymes (Said et al 2010;Lone et al 2017). These all stressors ultimately roots in inevitable damages on acrosome (Balamurugan et al 2018), head membrane (Hsieh et al 2006;Gadea et al 2013), tail and also aggravates premature capacitation (Shah et al 2017;Yadav et al 2017), lipid peroxidation (Khodaei et al 2016;Kumar et al, 2019b), which unduly compromise the quality and fertility of frozen-thawed sperm. The reduction in quality and concomitant fertility arises due to spermatozoa disintegration as a consequence of anatomical and biochemical destruction of subcellular organelles and alterations in epigenetic profile (Chatterjee et al 2017).…”

Section: Research Articlementioning

confidence: 99%

Time Dependent Impact of Reactive Oxidants on Seminal Attributes of Murrah Bull during Cryopreservation and Storage

Upadhyay¹,

Roy²,

Pandita³

et al. 2021

IJAR

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Background: Cryopreservation is an invaluable technique yet it is also known to be detrimental to sperm function and fertility due to cryo-injury and concomitant generation of reactive oxidants. During laboratory manipulation for the cryopreservation and freeze-thaw process, spermatozoa undergo osmotic stress, ionic imbalance, metabolic decoupling, membrane phase transition, destabilization of the cytoskeleton and antioxidant depletion which communally hampers the semen quality.Methods: With the aim of determining implications of cryopreservation and storage, semen samples were collected by artificial vagina technique from 12 Murrah bulls and subsequently examined at 0 hour (before cryopreservation) and at 24 hour, 1 month and 2 month of storage for various seminal attributes. Simultaneously seminal plasma was separated and preserved at -20oC till the analysis of biochemical indicators of semen quality viz., nitric oxide (NO), total antioxidant quantity (TAC) and lipid peroxidation status (TBARS). Result: A sharp reduction (p less than 0.01) in the semen quality was observed only at 24 h after cryopreservation except for viability. Significant reduction (p less than 0.05) in viable counts was observed up to 1 month interval. The capacitated sperm percentage was greater (p less than 0.01) in the cryopreserved semen as compared to fresh ejaculate. The mean ± SE levels of NO (μmol/L), TAC and TBARS (Units/ml) was 2.31±0.27, 0.73±0.04 and 1.11±0.16 respectively in seminal plasma of neat semen stored at -20oC, while the values in the extended seminal plasma after cryopreservation was 2.37±0.31, 0.44±0.03 and 0.65±0.03 respectively. So it can be inferred that most of the damage encountered by spermatozoa is during the initial period of freezing, but the damage associated by various stressors cannot be ignored.

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Effect of cholesterol loaded cyclodextrin supplementation on tyrosine phosphorylation and apoptosis like changes in frozen thawed Hariana bull spermatozoa

Cited by 33 publications

References 35 publications

Effect of various levels of dissolved oxygen on reactive oxygen species and cryocapacitation‐like changes in bull sperm

Effect of various levels of dissolved oxygen on reactive oxygen species and cryocapacitation‐like changes in bull sperm

Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview

Time Dependent Impact of Reactive Oxidants on Seminal Attributes of Murrah Bull during Cryopreservation and Storage

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