Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview

Upadhyay, Vishwa Ranjan; Ramesh, V.; Dewry, Raju Kumar; Kumar, Gaurav; Raval, Kathan; Patoliya, Priyanka

doi:10.1111/and.14154

Cited by 25 publications

(24 citation statements)

References 200 publications

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“…Semen cryopreservation causes the sperm to undergo considerable biophysical and biochemical changes, including alterations to membranes and their constituent parts, resulting in decreased post-thaw sperm motility and fertilization ability ( 36 , 37 ). However, semen from different individuals had the various capacity to protect against freezing damage ( 38 – 41 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of lipidomic change in goat sperm after cryopreservation

Wang²,

Wang³

et al. 2022

Front. Vet. Sci.

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The current study aimed to detect the relationship between the spermatozoa cryotolerance and the post-thawed sperm lipidome. Ejaculates from 20 goats, and performed a uniform frozen-thawed procedure in this study. According to the total motility of thawed sperm of goats, semen samples were classified into HF group (High Freezers, n = 8) with >60% total motility and LF group (Low Freezers, n = 8) with < 45% total motility. The lipidomic analysis based on UHPLC-MS/MS was utilized to investigate the relationship between sperm cryotolerance and their lipid metabolites expression. The results showed that the cryotolerance of sperm from different individual goats were in great variation. The total motility of post-thawed sperm in HF group (60.93 ± 2.43%) is significantly higher than that in LF group (34.04 ± 3.41%, P < 0.01). And the post-thawed sperm in HF group exhibited significantly higher plasma membrane (59.06 ± 2.34%) and acrosome integrity (62.93 ± 1.15%) than that in LF group (34.06 ± 4.85%, 44.92 ± 2.19% respectively, P < 0.01). The total of 29 lipid subclasses and 1,133 lipid molecules in the post-thawed goat sperm were identified by lipidomics analysis. The lipid content of thawed sperm in HF group was higher than that in LF group, the lipid profile in HF group was significantly separated from LF group, which indicated that the difference in lipid composition and lipid metabolism mode of sperm between the two groups was existed, especially the expression of phosphatidylcholine and triglyceride molecules. In conclusion, the cryotolerance of sperm from different individual goats were in great variation. Sperm with high cryotolerance may be able to uptake more lipids during cryopreservation. The increase in phosphatidylcholine and triglyceride content of thawed. Sperm may relate to more active lipid anabolic processes.

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Section: Discussionmentioning

confidence: 99%

Evaluation of lipidomic change in goat sperm after cryopreservation

Wang²,

Wang³

et al. 2022

Front. Vet. Sci.

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“…2 Oxidative stress occurs as the result of unavoidable imbalances between oxidants and antioxidants in a given system. 3 Spermatozoa are more prone to oxidative damage as cytoplasmic components of antioxidants are less or shed off during terminal stages of differentiation, and second, semen is extended which leads to antioxidants dilution present in seminal plasma during the cryopreservation process. 4 Excess formation of reactive oxygen species (ROS) causes lipid peroxidation, loss of mitochondrial membrane potential, and DNA damage, which results in poor post-thaw semen quality.…”

Section: Introductionmentioning

confidence: 99%

“…Stresses such as mechanical stress (ice crystal formations), cold shock, osmotic stress, and oxidative stress are experienced by spermatozoa during this process 2 . Oxidative stress occurs as the result of unavoidable imbalances between oxidants and antioxidants in a given system 3 . Spermatozoa are more prone to oxidative damage as cytoplasmic components of antioxidants are less or shed off during terminal stages of differentiation, and second, semen is extended which leads to antioxidants dilution present in seminal plasma during the cryopreservation process 4 .…”

Section: Introductionmentioning

confidence: 99%

Supplementation of Mito TEMPO and acetovanillone in semen extender improves freezability of buffalo spermatozoa

et al. 2022

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Background: Oxidative stress is one of the leading factors responsible for poor postthaw semen quality because of overproduction of reactive oxygen species (ROS) over neutralizing antioxidants present in semen. Mainly two ROS generation sites are present in spermatozoa, that is, mitochondria and plasma membrane. Therefore, the idea of targeting these specific sites for minimization of ROS production with the compounds having known mechanism of actions was built up as a core for this research.Objective: Present study was done to investigate the effects of Mito TEMPO and acetovanillone individually and in combination on freezability of buffalo spermatozoa. Materials and Methods:For the experiment, semen extender was supplemented with Mito TEMPO (50 μM), acetovanillone (50 μM), and a combination of Mito TEMPO + acetovanillone (50 μM+ 50 μM), designated as Group II, Group III, and Group IV, respectively. Control group without any supplementation was designated as Group I. A total of 24 ejaculates with individual progressive motility (IPM) of ≥70% were selected for the study. After final dilution, filling-sealing of straws, equilibration, and freezing were done as per the standard procedure. Semen samples were evaluated for IPM, plasma membrane integrity, lipid peroxidation, total antioxidant capacity (TAC), and cholesterol to phospholipids (C/P) ratio at both fresh and post-thaw stages. Evaluation of ROS, mitochondrial membrane potential (MMP), capacitation status (CTC assay), and in vitro fertility potential were conducted only on frozen-thawed samples.Results: The addition of Mito TEMPO (50 μM) and acetovanillone (50 μM) individually and in combination significantly (p < 0.05) improved post-thaw semen quality in terms of IPM, plasma membrane integrity, TAC, cholesterol content, C/P ratio, MMP, Chlortetracycline (CTC)-Full (F) pattern, and zona binding ability of buffalo spermatozoa, while significantly (p < 0.05) reduced ROS production, lipid peroxidation, and capacitation like changes as compared to the control group.Discussion: As Mito TEMPO acts as an SOD mimetic and also detoxifies ferrous iron at the mitochondria level, it aids in neutralization of excessive ROS production and minimizes oxidative stress-related damages that enhances the antioxidant potential of

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can be damaged during freeze-thawing and, as a result, their quality, function and fertilizing ability can be impaired (Adamkovicova et al, 2016;Díaz et al, 2019). This detrimental impact is the consequence of different processes that take place during freezing and thawing, such as the formation of ice microcrystals, which may induce mechanical damage to biological membranes; and the generation of excessive amounts of reactive oxygen species, which may increase DNA fragmentation and induce lipid peroxidation thus altering sperm structure and function (Upadhyay et al, 2021;Waterhouse et al, 2010). Yet, preserving mitochondrial functionality during cryopreservation is crucial for maintaining sperm fertilizing ability and guaranteeing the reproductive success (Amaral et al, 2013;Khan et al, 2021).In recent years, different strategies aimed at minimizing mitochondrial cryodamage, such as the enrichment of freezing media with antioxidants, proteins or cryoprotective agents (Hezavehei et al, 2018).

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mentioning

confidence: 99%

Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm

Algieri

Blanco-Prieto

Llavanera

et al. 2022

Reprod Domestic Animals

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can be damaged during freeze-thawing and, as a result, their quality, function and fertilizing ability can be impaired (Adamkovicova et al., 2016;Díaz et al., 2019). This detrimental impact is the consequence of different processes that take place during freezing and thawing, such as the formation of ice microcrystals, which may induce mechanical damage to biological membranes; and the generation of excessive amounts of reactive oxygen species, which may increase DNA fragmentation and induce lipid peroxidation thus altering sperm structure and function (Upadhyay et al., 2021;Waterhouse et al., 2010). Yet, preserving mitochondrial functionality during cryopreservation is crucial for maintaining sperm fertilizing ability and guaranteeing the reproductive success (Amaral et al., 2013;Khan et al., 2021).In recent years, different strategies aimed at minimizing mitochondrial cryodamage, such as the enrichment of freezing media with antioxidants, proteins or cryoprotective agents (Hezavehei et al., 2018). Red-light stimulation could be a potential strategy to improve sperm cryotolerance, as this procedure has been reported

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Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview

Cited by 25 publications

References 200 publications

Evaluation of lipidomic change in goat sperm after cryopreservation

Evaluation of lipidomic change in goat sperm after cryopreservation

Supplementation of Mito TEMPO and acetovanillone in semen extender improves freezability of buffalo spermatozoa

Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm

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