Effect of chlorine dioxide in reduction of harmful microbes on fermented hot pepper paste

Jung, Kyung-Mi; Lee, Gi-Woo; Cho, Mi-Ae; Song, Kyoung-Ju; Jung, Suk‐Yul

doi:10.1007/s10068-019-00606-8

Cited by 2 publications

(3 citation statements)

References 8 publications

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“…Bioethanol fermentation facilities are inherently prone to bacterial contamination found in raw materials such as corn mash and process water [ 4 , 6 , 28 , 29 ] which is difficult to eradicate from facilities [ 30 , 31 ] even with chemical treatments such as hop acids and chlorine-based oxides [ 9 , 10 ]. LAB species, in particular, L. fermentum , have been found to be one of the primary contaminants that thrive under the conditions typically found in bioethanol fermentation, such as low pH, high glucose concentration, and anaerobic conditions, and can have a negative impact on ethanol-producing yeast, leading to unpredictable acute stuck fermentation [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…More specifically, Limosilactobacillus fermentum and phylogenetically related heterofermentative LAB species have been identified as major culprits causing stuck fermentation across bioethanol facilities [ 6 , 8 ]. To minimize contamination at the facilities, hop acids, chlorine gas and antibiotics are often used prophylactically to treat active contamination [ 9 – 11 ]. Antibiotic residues in distillery products, such as distillers dried grains have been reported, raising concerns on potential antibiotic resistance and contamination in animal feed [ 12 , 13 ]…”

Section: Introductionmentioning

confidence: 99%

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Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation

Patel,

Lu,

Liu

et al. 2023

Biotechnol Biofuels

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Background Traditional bioethanol fermentation industries are not operated under strict sterile conditions and are prone to microbial contamination. Lactic acid bacteria (LAB) are often pervasive in fermentation tanks, competing for nutrients and producing inhibitory acids that have a negative impact on ethanol-producing yeast, resulting in decreased yields and stuck fermentations. Antibiotics are frequently used to combat contamination, but antibiotic stewardship has resulted in a shift to alternative antimicrobials. Results We demonstrate that endolysin LysMP, a bacteriophage-encoded peptidoglycan hydrolase, is an effective method for controlling growth of LAB. The LysMP gene was synthesized based on the prophage sequence in the genome of Limosilactobacillus fermentum KGL7. Analysis of the recombinant enzyme expressed in E. coli and purified by immobilized metal chelate affinity chromatography (IMAC) showed an optimal lysis activity against various LAB species at pH 6, with stability from pH 4 to 8 and from 20 to 40 °C up to 48 h. Moreover, it retains more than 80% of its activity at 10% ethanol (v/v) for up to 48 h. When LysMP was added at 250 µg/mL to yeast corn mash fermentations containing L. fermentum, it reduced bacterial load by at least 4-log fold compared to the untreated controls and prevented stuck fermentation. In comparison, untreated controls with contamination increased from an initial bacterial load of 1.50 × 107 CFU/mL to 2.25 × 109 CFU/mL and 1.89 × 109 CFU/mL after 24 h and 48 h, respectively. Glucose in the treated samples was fully utilized, while untreated controls with contamination had more than 4% (w/v) remaining at 48 h. Furthermore, there was at least a fivefold reduction in lactic acid (0.085 M untreated contamination controls compared to 0.016 M treated), and a fourfold reduction in acetic acid (0.027 M untreated contamination controls vs. 0.007 M treated), when LysMP was used to treat contaminated corn mash fermentations. Most importantly, final ethanol yields increased from 6.3% (w/v) in untreated contamination samples to 9.3% (w/v) in treated contamination samples, an approximate 50% increase to levels comparable to uncontaminated controls 9.3% (w/v). Conclusion LysMP could be a good alternative to replace antibiotics for mitigation of LAB contamination in biofuel refineries.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation

Patel,

Lu,

Liu

et al. 2023

Biotechnol Biofuels

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“…. Chlorine dioxide is 10 times more soluble in water than sodium hypochlorite, which is less reactive with organics and used as a disinfectant for food (Jung et al, 2019). ClO 2 gas is an effective disinfectant agent with strong oxidization ability and a broad biocidal spectrum (Gómez-López et al, 2009;Wang et al, 2016).…”

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confidence: 99%

Inhibition of Clinical Nosocomial Bacteria by Chlorine Dioxide

Jung

2019

BSL

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Chlorine dioxide is an effective chemical to inhibit the growth of bacteria and viruses or to disinfect infected areas. In this study, the effects of chlorine dioxide on several bacteria in hospitals were analyzed. Alloiococcus otitis, Kocuria rosea, Leuconostoc mesenteroides spp. and Staphylococcus lentus as gram-positive bacteria and Acinetobacter lwoffii, Aeromonas salmonicida, Brucella melitensis, Oligella ureolytica as gram-negative bacteria were done for the inhibitory analysis. The growth and morphology of the bacteria were analyzed by placing a plastic stick which was called "FarmeTok (medistick/ Puristic)" provided by Purgofarm, co, Ltd. to release ClO 2 (13 ppmv/hr) next to the plate where the bacteria were incubated for 24 hours. Less than 10 bacterial colonies were evaluated as having 99% inhibitory effect. The initial bacterial culture concentration of 0.5 McFaland turbidity was good for analyzing the chlorine dioxide inhibitory effect. All bacteria could be easily counted post 24 hr co-incubation with ClO 2 , but A. otitis and A. lwoffii without ClO 2 gas were not countable due to very dispersed colony types which were not affected for result analysis. As shown in this study, the FarmeTok plastic stick, which discharges chlorine dioxide at 13 ppmv / hour, was evaluated to be sufficient to suppress the above bacteria in the hospital. Bacteria existing in the clinic such as this hospital will be used as a data to inhibit the growth of bacteria by using ClO 2 , and molecular biology analysis using the gene of bacteria will be possible in the future rather than inhibiting the growth of bacteria itself.

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Effect of chlorine dioxide in reduction of harmful microbes on fermented hot pepper paste

Cited by 2 publications

References 8 publications

Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation

Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation

Inhibition of Clinical Nosocomial Bacteria by Chlorine Dioxide

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