Effect of Chitosan Supplemented Diet on Survival, Growth, Feed Utilization, Body Composition & Histology of Sea Bass (Dicentrarchus labrax)

Abstract

The effect of chitosan incorporated into feed formulation on the growth, feed utilization, body composition and histological response of sea bass (Dicentrarchus labrax) was investigated. Final fish weight, feed conversion ratio (FCR), specific growth rate (SGR), protein efficiency ratio (PER), protein productive value (PPV), and energy utilization (EU) of sea bass feed chitosan supplemented diet and the control diet were determined at the end of experiment. Data presents mean ± SD from triplicate determination (n = 3) for 75 days feeding trial. Different concentrations (0.5, 1.0, 2.0, 3.0 & 4.0) and control without chitosan incorporated with total fish feed in the form of dry diets were fed for 75 days. Average final weight (FW) was significantly higher at diet 3 (1 g•kg −1 chitosan) and the lowest in control fish group, specific growth rate (SGR) was significant at diet 3. Also, protein efficiency ratio (PER), protein productive value (PPV), and energy utilization (EU) showed the same trend. The chemical body composition, crude protein, dry matter, crude fat and ash were significant (P ≤ 0.01) different compared to control groups. The present investigation suggested that the chitosan incorporated into diets of sea bass fish certainly enhanced the non specific responses and reduced mortality and also improved the growth performance of fish.

Evaluation of dietary chitosan effects on growth performance, immunity, body composition and histopathology of Nile tilapia (Oreochromis niloticus) as well as the resistance toStreptococcus agalactiaeinfection

“…These results are in harmony withMeng, Wang, Wan, Xu, and Wang (2017),Zhou et al (2016),Zaki, Salem, Gaber, and Nour (2015),Niu et al (2011), Cha et al (2008, andGopalakannan and Arul (2006) in Nile tilapia, gibel carp, sea bass fish, post-larval shrimp, olive flounder and common fibres, Streptococcus agalactiaechallenged group (d and e) showing brain malacia (arrow) associated with bacterial colonies (arrowheads) (e indicates bacterial colonization), challenged fish and supplemented with diet contained chitosan (3 g/kg) (f) revealing microcystic formation with associated with perivascular and pericellular oedema (arrow), challenged fish and supplemented with diet contained chitosan (5 g/kg)(g) showing scanty spongiosis features (arrow). These results are in harmony withMeng, Wang, Wan, Xu, and Wang (2017),Zhou et al (2016),Zaki, Salem, Gaber, and Nour (2015),Niu et al (2011), Cha et al (2008, andGopalakannan and Arul (2006) in Nile tilapia, gibel carp, sea bass fish, post-larval shrimp, olive flounder and common fibres, Streptococcus agalactiaechallenged group (d and e) showing brain malacia (arrow) associated with bacterial colonies (arrowheads) (e indicates bacterial colonization), challenged fish and supplemented with diet contained chitosan (3 g/kg) (f) revealing microcystic formation with associated with perivascular and pericellular oedema (arrow), challenged fish and supplemented with diet contained chitosan (5 g/kg)(g) showing scanty spongiosis features (arrow).…”

“…Streptococcus agalactiae infection was associated with thrombo-haemorrhagic and inflammatory lesions within the brain, ocular, hepatic and renal tissue (Laith et al, 2017). Chitosan supplementation improved the intestinal absorptive capacity through increased intestinal villi length (Najafabad et al, 2016;Zaki et al, 2015). The histopathological findings within the different tissue of challenged fish that treated with chitosan revealed a marked decrease in the cephalic, hepatic and renal lesions.…”

Effects of dietary chitosan supplementation on farmed fish; a review

“…They have found that the supplementation of chitosan in diets of sea bass ( Dicentrarchus labrax ) at concentrations of 1 and 2 g kg −1 diet enhanced the growth performance, feed utilization parameters as well as the intestinal villi height (Zaki et al . ). This finding is supported by Shi et al .…”

“…Zaki et al . () reported that the chitosan‐supplemented diets of sea bass ( Dicentrarchus labrax ) at low and moderate concentrations enhanced fish growth. However, at higher concentrations, the growth depression occurred, accompanied by histological changes in the intestine.…”

“…To date, no adverse or inhibitory effects are reported at low or moderate concentrations of chitosan in aquaculture applications (Niu et al 2011;Shi-bin & Hong 2012;Zaki et al 2015). Zaki et al (2015) reported that the chitosan-supplemented diets of sea bass (Dicentrarchus labrax) at low and moderate concentrations enhanced fish growth. However, at higher concentrations, the growth depression occurred, accompanied by histological changes in the intestine.…”