Effect of Chitosan Incorporated with Cumin and Eucalyptus Essential Oils As Antimicrobial Agents on Fresh Chicken Meat

Abstract

In this study, cumin and eucalyptus essential oils were prepared and analyzed by gas chromatography-flame ionization detector. Its antibacterial effects were screened using the microdilution method for Listeria monocytogenes, Salmonella typhi, Streptococcus pyogenes and Shigella dysenteriae. The effect of the three concentrations of essential oils (0.5, 1 and 2% w/v) with 2% chitosan at 4 ± 1C temperature and storage time of up to 9 days were evaluated on the microbial quality of chicken meat. Changes in total mesophilic bacterial count (TMBC), lactic acid bacteria, Enterobacteriaceae, mold and yeast counts, and sensory properties were evaluated. The main compounds in cumin were 1,8-cineol (26.75%) and cuminaldehyde (17.1%) and the main compounds in eucalyptus were 1,8-cineol (77.32%) and limonene (8.39%). The essential oils have antibacterial effects on the four examined bacteria. In all of the treatment groups, a decrease of TMBC up to the sixth day and lactic acid bacteria, Enterobacteriaceae, mold and yeast up to the ninth day (P < 0.05) were observed. Overall acceptance rate in the chicken meat containing chitosan with 0.5% cumin essential oil created a better sense. Hence, the use of chitosan combined with cumin and eucalyptus might be suggested as an antibacterial packaging to extend the shelf life and as a flavor enhancer for chicken meat. PRACTICAL APPLICATIONSThe results suggested that because chitosan film incorporated with cumin and eucalyptus can reduce meat spoilage losses and improve nutritional value, it can be used as an active packaging in the meat industry.

Chemical Composition, Antioxidant and Antibacterial Activity ofBunium persicum,Eucalyptus globulus, and Rose Water on Multidrug-ResistantListeriaSpecies

“…The MIC and MBC values were 5.625 and 11.25 mg/mL, respectively. 42 The difference antimicrobial properties of extracts and essential oils against Listeria spp may be linked to inoculation amount of bacteria, incubation time as experimental conditions, and the sources of extracts and essential oils and their chemical composition. 40 The results of this study showed, increase in the optical density values at 260 nm of L monocytogenes and L grayi treated with B persicum and E globulus essential oils over time, which can result in changes of cell membrane structure.…”

Antimicrobial activity of chitosan incorporated with lemon and oregano essential oils on broiler breast meat during refrigerated storage

“…Despite the high potential of essential oils and their extract as food preservatives, ingesting these at high doses can induce serious problems, and their toxicity should be considered (Sharafati Chaleshtori et al , 2013b; Bahmani et al , 2014). Therefore, several researchers have suggested the concomitant use of different food preservation systems such as modified atmosphere packaging, high hydrostatic pressure, natural antibacterial agents and biopreservation (Sharafati Chaleshtori et al , 2016; Reichling et al , 2009).…”

Antibacterial Activity and Physical Properties of Fish Gelatin-Chitosan Edible Films Supplemented with D-Limonene

“…Many researchers have already focused on natural materials such as polysaccharides [1,2], proteins [3][4][5], lipids [6], or combinations of these materials [7]. Recently, many types of gelatin-chitosan edible films [8,9] and the films incorporated with essential oils have been studied [10][11][12]. The water-soluble chitosan, fish gelatin, and D-limonene stimulated our interests to design the multifunctional films because of the following advantages.…”