Effect of Chemically Modified 70 S RNA from Avian Myeloblastosis Virus (AMV) upon the Activity of AMV DNA Polymerase

Abstract

Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent e-adenosine (EA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(EA) RNA was maintained intact and was inactive as a template for the AMV DNA polymerase. Furthermore, it inhibited the reaction catalyzed by AMV polymerase when 70S RNA was used as template-primer and had no effect on the two tested bacterial polymerases. Protection against the 70S (EA) RNA inhibition was observed when 70S RNA was primed with oligo(dT) indicating preference of the polymerase for the oligo(dT) primed regions.