Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells

Zayed, Gamal; Kamal, Islam; Abdelhafez, Wael A.; Alsharif, Fahd M.; Amin, Mohamed A.; Shaykoon, Montaser Sh. A.; Sarhan, Hatem A.; Abdelsalam, Ahmed M.

doi:10.1007/s11095-018-2393-6

Cited by 15 publications

(12 citation statements)

References 59 publications

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“…It is well recognized that drugs may be physically adsorbed or covalently attached to a metallic nanomaterial surface (45). In the present paper, the developed nanoparticles were further loaded with PHT through a physical adsorption approach and their wound-healing efficacy was evaluated.…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis, Characterization, and Wound-Healing Activity of Phenytoin-Loaded Copper Nanoparticles

et al. 2020

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Wound-healing is a very complex and evolutionary process that involves a great variety of dynamic steps. Although different pharmaceutical agents have been developed to hasten the woundhealing process, the existing agents are still far from optimal. The present work aimed to prepare and evaluate the wound-healing efficacy of phenytoin-loaded copper nanoparticles (PHT-loaded CuNPs). CuNPs were biosynthesized using licorice aqueous extract. The prepared CuNPs were loaded with PHT by adsorption, characterized, and evaluated for wound-healing efficiency. Results showed that both plain and PHT-loaded CuNPs were monodisperse and exhibited a cubic and hexagonal morphology. The mechanism by which PHT was adsorbed on the surface of CuNPs was best fit by the Langmuir model with a maximum loaded monolayer capacity of 181 mg/g. The kinetic study revealed that the adsorption reaction followed the pseudo-second order while the thermodynamic parameters indicated that the adsorption process was physical in nature and endothermic, and occurred spontaneously. Moreover, the in vivo wound-healing activity of PHT-loaded CuNP impregnated hydroxypropylmethyl cellulose (HPMC) gel was carried out using an excisional wound model in rats. Data showed that PHT-loaded CuNPs accelerated epidermal regeneration and stimulated granulation and tissue formation in treated rats compared to controls. Additionally, quantitative real-time polymerase chain reaction (RT-PCR) analysis showed that lesions treated with PHT-loaded CuNPs were associated with a marked increase in the expression of dermal procollagen type I and a decrease in the expression of the inflammatory JAK3 compared to control samples. In conclusion, PHT-loaded CuNPs are a promising platform for effective and rapid wound-healing.

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Section: Resultsmentioning

confidence: 99%

Biosynthesis, Characterization, and Wound-Healing Activity of Phenytoin-Loaded Copper Nanoparticles

et al. 2020

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“…Among them, pH-responsive drug carriers have been mainly investigated − since they are able to release the cargo drugs only at the site of the action in the acidic microenvironment of the diseased tissue and in the acidic organelles of cells. In this context, the the pH-cleavable hydrazone bond is an efficient linker between gold nanoparticle carriers and drugs, especially the ones containing a ketone group such as DOX. − Specifically, this linkage is fairly stable at neutral pH 7.4, with low or no DOX release expected at physiological conditions within the circulation, but, on the contrary, it is cleavable in the lower-pH environments of cellular organelles. , Thus, by designing such delivery nanosystems, it will be possible not only to selectively deliver drugs by keeping them attached to the carrier during systemic circulation but also to provide a controlled release in acidic media of the tumoral cells. − However, it is imperative to monitor the intracellular dynamic distribution and release of drugs. ,, Thus, TPE-FLIM imaging is the ideal tool for tracking the DOX intracellular dynamics since it can detect even slight changes in its distribution.…”

Section: Introductionmentioning

confidence: 99%

“…27−29 Specifically, this linkage is fairly stable at neutral pH 7.4, with low or no DOX release expected at physiological conditions within the circulation, but, on the contrary, it is cleavable in the lower-pH environments of cellular organelles. 28,30 Thus, by designing such delivery nanosystems, it will be possible not only to selectively deliver drugs by keeping them attached to the carrier during systemic circulation but also to provide a controlled release in acidic media of the tumoral cells. 31−34 However, it is imperative to monitor the intracellular dynamic distribution and release of drugs.…”

Section: Introductionmentioning

confidence: 99%

Intracellular Dynamic Disentangling of Doxorubicin Release from Luminescent Nanogold Carriers by Fluorescence Lifetime Imaging Microscopy (FLIM) under Two-Photon Excitation

Suarasan

Craciun

Licărete

et al. 2019

ACS Appl. Mater. Interfaces

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There is still a lack of available techniques to follow noninvasively the intracellular processes as well to track or disentangle various signals from the therapeutic agents at the site of action in the target cells. We present here the assessment of the intracellular kinetics of doxorubicin (DOX) and gold nanoparticle (AuNP) carriers by mapping simultaneously fluorescence and photoluminescence signals by fluorescence lifetime imaging microscopy under two-photon excitation (TPE-FLIM). The new nano–chemotherapeutic system AuNPs@gelatin–hyd–DOX has been fabricated by DOX loading onto the surface of gelatin-biosynthesized AuNPs (AuNPs@gelatin) through a pH-sensitive hydrazone bond. The successful loading of DOX onto the AuNPs was studied by spectroscopic methods and steady-state fluorescence, and the nanosystem pH-responsive character was validated under simulated biological conditions at different pH values (i.e., pH 4.6, 5.3, and 7.4). Considering that the fluorescence lifetime of DOX molecules at a specific point in the cell is a reliable indicator of the discrimination of the different states of the drug in the internalization path, i.e., released versus loaded, the kinetics of AuNPs@gelatin–hyd–DOX cellular uptake and DOX release was compared to that of free DOX, resulting in two different drug internalization pathways. Finally, cell viability tests were conducted against NIH:OVCAR-3 cell line to prove the efficiency of our chemotherapeutic nanosystem. TPE-FLIM technique could be considered promising for noninvasive, high-resolution imaging of cells with improved capabilities over current one-photon-excited FLIM.

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“…Zayed et al [ 56 ] exploited the in vitro drug release of doxorubicin hydrochloride (DOX·HCl) chemically bonded to AuNPs using breast cancer cells. They conjugated DOX·HCl via a hydrazone bond to the carboxyl functional group of a pre-formed polymer (undecanethiol-polyethylene glycol hydrazide).…”

Section: Overview: Green Biosynthesis Of Metallic Nanoparticlesmentioning

confidence: 99%

Green Metallic Nanoparticles for Cancer Therapy: Evaluation Models and Cancer Applications

et al. 2021

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Metal-based nanoparticles are widely used to deliver bioactive molecules and drugs to improve cancer therapy. Several research works have highlighted the synthesis of gold and silver nanoparticles by green chemistry, using biological entities to minimize the use of solvents and control their physicochemical and biological properties. Recent advances in evaluating the anticancer effect of green biogenic Au and Ag nanoparticles are mainly focused on the use of conventional 2D cell culture and in vivo murine models that allow determination of the half-maximal inhibitory concentration, a critical parameter to move forward clinical trials. However, the interaction between nanoparticles and the tumor microenvironment is not yet fully understood. Therefore, it is necessary to develop more human-like evaluation models or to improve the existing ones for a better understanding of the molecular bases of cancer. This review provides recent advances in biosynthesized Au and Ag nanoparticles for seven of the most common and relevant cancers and their biological assessment. In addition, it provides a general idea of the in silico, in vitro, ex vivo, and in vivo models used for the anticancer evaluation of green biogenic metal-based nanoparticles.

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Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells

Abstract: Chemical binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.

Cited by 15 publications

References 59 publications

Biosynthesis, Characterization, and Wound-Healing Activity of Phenytoin-Loaded Copper Nanoparticles

Biosynthesis, Characterization, and Wound-Healing Activity of Phenytoin-Loaded Copper Nanoparticles

Intracellular Dynamic Disentangling of Doxorubicin Release from Luminescent Nanogold Carriers by Fluorescence Lifetime Imaging Microscopy (FLIM) under Two-Photon Excitation

Green Metallic Nanoparticles for Cancer Therapy: Evaluation Models and Cancer Applications

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