Effect of cheddar cheese consumption on plasma conjugated linoleic acid concentrations in men

Huang, Yi‐Chia; Luedecke, L.O.; Shultz, Terry D.

doi:10.1016/s0271-5317(05)80176-9

Cited by 81 publications

(35 citation statements)

References 43 publications

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“…Britton et al (1992) have shown comparable increases in the incorporation of cis-9,trans-11 isomer of CLA into plasma phospholipids of human subjects as a result of increased consumption of food products rich in CLA for 3 weeks. Huang et al (1994) demonstrated that the cis-9,trans-11 isomer of CLA increased by 19 -27 % as a result of increased intake of dairy products in human subjects. There was a significant reduction in eicosapentaenoic acid (20:5) of total fatty acid composition of plasma lipids in the 80 : 20 CLA group, suggesting that increased incorporation of cis-9,trans-11 CLA may be at the expense of n-3 polyunsaturated fatty acids.…”

Section: Fatty Acid Composition Of Total Plasma Lipidsmentioning

confidence: 99%

The effect of dietary supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects

et al. 2002

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Conjugated linoleic acid (CLA) refers to a group of positional and geometric isomers of linoleic acid. Studies using animal models have shown that CLA reduces adiposity, improves plasma lipoprotein metabolism and insulin sensitivity and reduces arteriosclerosis. Whilst CLA may have therapeutic potential with regard to coronary artery disease risk factors in human subjects, there has been little investigation into its effects in human subjects. This current study investigated the effects of dietary supplementation using two isomeric blends of CLA on triacylglycerol (TAG)-rich lipoprotein metabolism and reverse cholesterol transport in human subjects and evaluates whether CLA modulated cardiovascular disease risk factors. Fifty-one normolipidaemic subjects participated in this randomised double-blind placebo-controlled intervention trial. Subjects were randomly assigned to receive 3 g cis-9,trans-11 -trans-10,cis-12 isomeric blend (50 : 50) or a cis-9,trans-11-trans-10,cis-12 isomeric blend (80 : 20) CLA or linoleic acid (control)/d for 8 weeks. The 50 : 50 CLA isomer blend significantly reduced (P# 0·005) fasting plasma TAG concentrations. The 80 : 20 CLA isomer blend significantly reduced (P# 0·05) VLDL-cholesterol concentrations. CLA supplementation had no significant effect on LDL-cholesterol, HDL-lipid-protein composition or reverse cholesterol transport. CLA supplementation had no effect on body weight, plasma glucose and insulin concentrations. Fatty acid analysis revealed that the cis-9,trans-11 CLA isomer was incorporated into total plasma lipids following supplementation with both isomeric blends of CLA. The present study demonstrates that CLA supplementation significantly improves plasma TAG and VLDL metabolism in human subjects. The study confirms that some of the cardio-protective effects of CLA that were shown in animal studies are relevant to man.Conjugated linoleic acid: Triacylglycerol: Very-low-density lipoprotein: Low-density lipoprotein: High-density lipoprotein Conjugated linoleic acid (CLA) is the term used to describe a mixture of positional and geometric isomers of linoleic acid, with conjugated double bonds, which may be of cis or trans configuration at positions 9 and 11 or 10 and 12 (Ha et al. 1987). CLA is a natural food constituent, produced by the intestinal flora of ruminant animals (Kepler et al. 1970) and is found in the lipid fraction of meat and dairy products. Chin et al. (1992) estimated the daily intake of CLA in human subjects to be 160 mg/d. Several animal studies have established that CLA has profound effects on lipoprotein metabolism and prevents dietinduced atherosclerosis. Lee et al. (1994) showed that CLA significantly reduced plasma triacylglycerol (TAG) and LDL-cholesterol concentrations and demonstrated that cholesterol deposition in the aorta was 30 % less in the CLA-fed rabbits. Nicolosi et al. (1997) demonstrated that CLA supplementation significantly reduced plasma TAG (2 28 %) and cholesterol (2 26 %) concentrations, which were associated with a significant redu...

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Section: Fatty Acid Composition Of Total Plasma Lipidsmentioning

confidence: 99%

The effect of dietary supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects

et al. 2002

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“…This group consumed large quantities of butter and ghee (heated, clarified butter oil), which, based on a high fat content (at least 80%) contain large amounts of the acid. Huang et al (205) fed 112 g (4 oz) of Cheddar cheese per day to male subjects. Plasma contents of rumenic acid were increased 19-27% without affecting C contents.…”

Section: Comments On Pufamentioning

confidence: 99%

Lipids in human milk

Jensen

1999

Lipids

475

437

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I have reviewed recent (March 1995-December 1997) papers on human milk lipids including many on fatty acid (FA) composition. The effects of maternal diets on the profiles are apparent. However, more data on the composition of milk lipids are needed. It is noteworthy that so few papers on milk FA composition have reported analyses using high-resolution gas-liquid chromatography columns. Two of these were on milk from women in North America. The diets in North America are varied and the number of analyses few. We do not have a reliable data base showing the ranges of biologically important acids. Except for the gangliosides, few new data on the other lipids appeared during this period.

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“…This was confirmed in a recent study wherein feeding VA (2% of diet, w/w) elicited a biological response in a rat mammary tumor model, reducing the total number of premalignant lesions in the rat mammary gland by approximately 50% in carcinogen-treated rats (21). Although CLA is present at relatively low concentrations in human adipose tissue, bile, duodenal juice, breast milk, and serum lipids (22)(23)(24), specific dietary intervention trials markedly increased the CLA content in human milk (25), plasma (26), and adipose tissue (27). Together, these observations suggest that increasing the amount of VA in the diet may enhance the pool of CLA in human tissues.…”

mentioning

confidence: 99%

Vaccenic acid (t11–18∶1) is converted to c9,t11‐CLA in MCF‐7 and SW480 cancer cells

Miller

McGrath

Stanton

et al. 2003

Lipids

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The aims of this study were to determine whether vaccenic acid (VA; t11-18:1) is converted to c9,t11-CLA in human mammary (MCF-7) and colon (SW480) cancer cell lines and whether VA influences cell viability and other CLA-bioresponsive markers. When cells were incubated in the presence of VA at concentrations of 5 to 20 microg/mL, both VA and c9,t11-CLA increased in cellular lipids in a dose-dependent manner. After 4 d of incubation of SW480 and MCF-7 cells with VA (20 microg/mL), c9,t11-CLA increased from undetectable levels to 8.57 and 12.14 g/100 g FAME in cellular lipids, respectively. VA supplementation for 4 d at 5, 10, and 15 microg/mL had no effect on cell growth, whereas 20 microg/mL significantly (P < 0.05) reduced cell growth in both cell lines. VA (20 microg/mL) treatment induced DNA fragmentation and significantly (P < 0.05) depleted cytosolic GSH levels in the SW480 cell line after 4 d of incubation, suggesting that apoptosis was the mode of cell death induced by VA. Both VA and c9,t11-CLA reduced (P < 0.05) total ras expression in SW480 cells. 14C-Arachidonic acid uptake into the MG fraction was significantly increased (P < 0.05) in both cell lines while uptake into the phospholipid fraction decreased in response to VA. VA treatment significantly (P < 0.05) increased 8-epi-prostaglandin F2alpha in both cell lines. The data indicate that growth suppression and cellular responses of both cells lines are likely mediated by VA desaturation to c9,t11-CLA via delta9-desaturase.

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Effect of cheddar cheese consumption on plasma conjugated linoleic acid concentrations in men

Cited by 81 publications

References 43 publications

The effect of dietary supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects

The effect of dietary supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects

Lipids in human milk

Vaccenic acid (t11–18∶1) is converted to c9,t11‐CLA in MCF‐7 and SW480 cancer cells

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