Glycidyl trimethyl ammonium chloride (Glytac) was used as a modifier for surface modification of jute fiber. Modifying effect factors such as Glytac concentration, sodium hydroxide concentration, temperature, and time were optimized as follows: Glytac concentration, 60 g/L; sodium hydroxide concentration, 20 g/L; temperature, 60 • C; and treatment time, 60 min. After the Glytac treatment, the zeta potential value on the fiber surface increased over a pH range of 3.5-9.5. Compared to the raw jute fibers, the modified jute fibers could be dyed with reactive dye using a small amount of salt and alkali and had higher K/S values under the same dye concentration range of 0.5-8. 0% (o.w.f.). Furthermore, the dyed Glytac-treated jute fibers had higher exhaustion, fixation, and total fixation and better washing fastness and dry rubbing fastness than those of raw jute fibers.