Effect of cell size on insulin secretion in human β-cells: a simulation study

Félix‐Martínez, Gerardo J.; Godínez‐Fernández, J. Rafael

doi:10.1007/s12553-019-00350-3

Cited by 3 publications

(3 citation statements)

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“…First, Ca 2+ microdomains in the vicinity of the Ca 2+ channels are considered to be the immediate fusion signal, and may not be well represented in the bulk cytosolic Ca 2+ concentration [ 53 , 54 ]. Based on recent work by Felix-Martinez et al [ 55 ], spatially resolved calcium concentrations can also be calculated, and lead to very significant gradients within the cytosol (they are also correlated with insulin secretion models [ 56 ]). For future studies, this would also raise questions about the assumption of a homogenised consideration of the Ca 2+ in the system of equations discussed here.…”

Section: Discussionmentioning

confidence: 99%

The Dynamics of Calcium Signaling in Beta Cells—A Discussion on the Comparison of Experimental and Modelling Data

Müller

Walkling

Seemann

et al. 2023

IJMS

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The stimulus–secretion coupling of the pancreatic beta cell is particularly complex, as it integrates the availability of glucose and other nutrients with the neuronal and hormonal input to generate rates of insulin secretion that are appropriate for the entire organism. It is beyond dispute however, that the cytosolic Ca2+ concentration plays a particularly prominent role in this process, as it not only triggers the fusion of insulin granules with the plasma membrane, but also regulates the metabolism of nutrient secretagogues and affects the function of ion channels and transporters. In order to obtain a better understanding of the interdependence of these processes and, ultimately, of the entire beta cell as a working system, models have been developed based on a set of nonlinear ordinary differential equations, and were tested and parametrized on a limited set of experiments. In the present investigation, we have used a recently published version of the beta cell model to test its ability to describe further measurements from our own experimentation and from the literature. The sensitivity of the parameters is quantified and discussed; furthermore, the possible influence of the measuring technique is taken into account. The model proved to be powerful in correctly describing the depolarization pattern in response to glucose and the reaction of the cytosolic Ca2+ concentration to stepwise increases of the extracellular K+ concentration. Additionally, the membrane potential during a KATP channel block combined with a high extracellular K+ concentration could be reproduced. In some cases, however, a slight change of a single parameter led to an abrupt change in the cellular response, such as the generation of a Ca2+ oscillation with high amplitude and high frequency. This raises the question as to whether the beta cell may be a partially unstable system or whether further developments in modeling are needed to achieve a generally valid description of the stimulus–secretion coupling of the beta cell.

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Section: Discussionmentioning

confidence: 99%

The Dynamics of Calcium Signaling in Beta Cells—A Discussion on the Comparison of Experimental and Modelling Data

Müller

Walkling

Seemann

et al. 2023

IJMS

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“…Authors An index to prioritize the preventive maintenance of medical equipment [1] Hernández Effect of cell size on insulin secretion in human β-cells: a simulation study [7] Félix-Martínez, G.J., Godínez-Fernández, J.R. Automated extraction of fine and coarse crackles by Independent Component Analysis [8] García, M.E.R., Villalobos, S.C., Villa, N.C. et al Developing a visual perimetry test based on eye-tracking: proof of concept [9] Martínez-González, E.A., Alba, A., Méndez, M.O.…”

Section: Paper Títlementioning

confidence: 99%

Introduction to the new special issue for the 2018 SOMIB / CNIB

Kun

2020

Health Technol.

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“…En los últimos años, en el Laboratorio de Biofísica e Ingeniería de Tejidos de la Universidad Autónoma Metropolitana, Unidad Iztapalapa, se ha llevado a cabo trabajo multidisciplinario enfocado en el estudio de la fisiopatología de la diabetes a nivel celular. Como ejemplo de esto, en 2014 se presentó un estudio de los posibles efectos de las alteraciones en el metabolismo de las células β humanas en la secreción de insulina [44]. Siguiendo la misma línea, se hicieron estudios de la contribución de las diferentes poblaciones de canales iónicos de la célula β humana en el patrón de actividad eléctrica [45], así como de la dinámica del Ca 2+ intracelular asociada durante experimentos electrofisiológicos [46].…”

Section: Antecedentesunclassified

Modelo computacional de la secreción pulsátil de insulina en islotes pancreáticos de tipo humano

Labastida

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Effect of cell size on insulin secretion in human β-cells: a simulation study

Cited by 3 publications

References 8 publications

The Dynamics of Calcium Signaling in Beta Cells—A Discussion on the Comparison of Experimental and Modelling Data

The Dynamics of Calcium Signaling in Beta Cells—A Discussion on the Comparison of Experimental and Modelling Data

Introduction to the new special issue for the 2018 SOMIB / CNIB

Modelo computacional de la secreción pulsátil de insulina en islotes pancreáticos de tipo humano

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