Effect of cations, pH and sulfate content on the viscosity and emulsifying activity of the Halomonas eurihalina exopolysaccharide

Calvo, C.; Martínez‐Checa, Fernando; Mota, Antonio J.; Béjar, Victoria; Quesada, Emilia

doi:10.1038/sj.jim.2900513

Cited by 71 publications

(40 citation statements)

References 27 publications

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“…The genus Halomonas has received increasing interest because of a number of reports describing species that produce significant quantities of EPS with high surface activity and/or rheological properties (Calvo et al, 1998Martinez-Checa et al, 2002;Pepi et al, 2005). With the aim to exploit their potential biotechnological uses, optimization of the culture conditions used to grow these organisms is an important initial step towards maximizing the production yield of these types of polymers whilst also aiming to improve on their functionality (Giavasis et al, 2000;Laws et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Yield and physicochemical properties of EPS from Halomonas sp. strain TG39 identifies a role for protein and anionic residues (sulfate and phosphate) in emulsification of n‐hexadecane

Gutiérrez

Morris

Green

2009

Biotech & Bioengineering

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In this study, we investigated the yield and physicochemical properties of the high molecular weight extracellular polymeric substance (HMW-EPS) produced by Halomonas sp. strain TG39 when grown on different types and ratios of substrates. Glucose (1% w/v) and a peptone/yeast extract ratio of 5.1 (0.6% w/v final concentration) yielded an EPS fraction (HMW-glucose) exhibiting the highest anionic activity (20.5) and specific emulsifying activity (EI24 = 100%) compared to EPS produced by cells grown on mannitol, sucrose, malt extract or no carbon source. The HMW-EPS fractions were capable of binding approximately 255-464 mg of methylene blue (MB) per gram of EPS, which represents the highest reported binding of MB by a bacterial EPS. A comparative evaluation of these properties to those of commercial hydrocolloids indicated that the combined effect of protein and anionic residues of the HMW-EPS contributed to its ability to emulsify n-hexadecane. Liquid chromatography revealed the HMW-glucose EPS to be a heterogeneous polymer with a polydispersity index of 1.8. This work presents evidence of a correlation between the anionic nature and protein content of bacterial EPS with its emulsifying qualities, and identifies EPS produced by strain TG39 as a high MB-binding bacterial sorbant with potential biotechnological application.

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Section: Introductionmentioning

confidence: 99%

Yield and physicochemical properties of EPS from Halomonas sp. strain TG39 identifies a role for protein and anionic residues (sulfate and phosphate) in emulsification of n‐hexadecane

Gutiérrez

Morris

Green

2009

Biotech & Bioengineering

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“…Although the market for polymers already has recourse to gums of remarkable quality, the diversity of micro-organisms being discovered offers excellent prospects of finding new EPS with new or better properties. The polymers found in our laboratory, for example, possess advantageous qualities not shared by many polysaccharides currently in use : they have simultaneous emulsifying and viscosifying activities ; some are able to gel at acidic pH ; they are quite resistant to high osmotic strength ; and are also thermostable (Bouchotroch et al, 2000 ;Calvo et al, 1998). We are carrying out a wide search aimed at isolating and Recently, the results of a taxonomic study of a group of 46 moderately halophilic, EPS-producing bacteria isolated from hypersaline environments in Morocco were reported.…”

Section: Introductionmentioning

confidence: 99%

Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium.

Bouchotroch¹,

Quesada²,

Moral³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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157

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Four moderately halophilic, exopolysaccharide-producing bacterial strains isolated from soil samples collected from a saltern at Asilah (Morocco) are reported. These four strains were initially considered to belong to the genus Halomonas. Their DNA GMC contents varied between 622 and 641 mol %. DNA-DNA hybridization revealed a considerable degree of DNA-DNA similarity amongst all four strains (755-808 %). Nevertheless, similarity with the reference strains of phylogenetically close relatives was lower than 40 %. 16S rRNA gene sequences were compared with those of other species of Halomonas and other Gram-negative bacteria and they were sufficiently distinct phylogenetically from other recognized Halomonas species to warrant their designation as a novel species. The name Halomonas maura sp. nov. is therefore proposed, with strain S-31 T (l CECT 5298 T l DSM 13445 T ) as the type strain. The fatty acid composition of strain S-31 T revealed the presence of 18 :1ω7c, 16:1ω7c/2-OH i15 :0 and 16 :0 as the major components. Growth rate analysis showed that strain S-31 T had specific cationic requirements for Na M and Mg 2M .

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“…A summary of such ndings is shown in Table 1, along with their identied bioemulsier compositions. Bacterial trains belonging to genera including Acinetobacter, 12 Aeribacillus, 13 Alcaligenes, 14 Amycolatopsis, 15 Azotobacter, 16 Bacillus, 17 Beijerinckia, 18 Corynebacterium, 19 Enterobacter, 20 Geobacillus, 13 Halomonas, 21 Klebsiella, 22 Myroides, 23 Pedobacter, 24 Propionibacterium, 25 Pseudomonas, 26 Solibacillus, 27 Streptomyces, 28 Variovorax, 29 have been reported to be bioemulsier producers (Table 1). To date, however, no strains belong to the genus of Exiguobacterium have been reported as bioemulsier producers.…”

Section: Introductionmentioning

confidence: 99%

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

et al. 2017

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In this study, Exiguobacterium N4-1P is reported as a bioemulsifier producer for the first time. The strain was found to be closely related to E. oxidotolerans strain T-2-2 T , E. antarcticum B7 T , and E. antarcticum B7 T with similarities of 99.47, 98.70 and 98.63%, respectively. Its phenotypic properties such as metabolic fingerprints, membrane composition, and cell morphology were determined. Different carbon sources were used for bioemulsifier production and diesel was confirmed to stimulate the yield effectively. The produced bioemulsifier is a complex mainly composed of lipopeptides with C16:0 (32.18%) and C18:0 (40.99%) as the primary fatty acids. The produced bioemulsifier could form emulsions effectively with diverse hydrocarbons. No foams were formed during the production and applications, which would facilitate its commercialization. The bioemulsifier was stable over a wide range of salinity (0-25%), pH (2-12), and temperature (below 50 C). Exiguobacterium N4-1P and the produced bioemulsifier fills knowledge gaps and has promising application potential in diverse fields, especially in environmental engineering.

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Effect of cations, pH and sulfate content on the viscosity and emulsifying activity of the Halomonas eurihalina exopolysaccharide

Cited by 71 publications

References 27 publications

Yield and physicochemical properties of EPS from Halomonas sp. strain TG39 identifies a role for protein and anionic residues (sulfate and phosphate) in emulsification of n‐hexadecane

Yield and physicochemical properties of EPS from Halomonas sp. strain TG39 identifies a role for protein and anionic residues (sulfate and phosphate) in emulsification of n‐hexadecane

Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium.

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

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