Effect of CaTiO₃–CaCO₃ prepared by alkoxide method on cell response

Abstract

In recent years, calcium titanate (CaTiO(3)) and carbon-containing materials have gained much attention in a number of biomedical material researches. To maximize the advantages of both materials, we developed a novel alkoxide method to get "calcium titanate with calcium carbonate" (CaTiO(3)-CaCO(3)). The objective was to evaluate the crystallinity and elemental composition of CaTiO(3)-CaCO(3) prepared by alkoxide method, CaTiO(3)-aC elaborated by modified thermal decomposition method, commercially-prepared CaTiO(3), and the effect of these materials on the bone marrow stromal cell. Hydroxyapatite was used as positive control material. We examined the cellular proliferation, osteoblastic differentiation, and mineralization of KUSA/A1 cells cultured with the materials. The results showed that CaTiO(3)-CaCO(3) and CaTiO(3)-aC contained evidence of calcium carbonate enhancing cell proliferation, osteoblastic differentiation, and mineralization. On the contrary, the commercially-prepared CaTiO(3) revealed absence of calcium carbonate with lower cell response than the other groups. The results indicated that calcium carbonate could play a key role in the cell response of CaTiO(3) material. In conclusion, our findings suggest that CaTiO(3)-CaCO(3) could be considered an important candidate as a biomaterial for medical and dental applications.

<i>In Vitro</i> Efficacy of CaCO₃ Content in CaTiO₃– CaCO₃ Composites for Bone Growth

“…It is well known that carbon-containing materials improve biological response as we showed during previous studies of our group, where we demonstrated that calcium carbonate in CaTiO 3 -CaCO 3 has a good osteoblastic response 22) . Hence, here we examined the eff ect of diff erent amounts of CaCO 3 combined with CaTiO 3 on cell proliferation and osteoblastic response.…”

“…6). Such eff ect was observed as well when KUSA/A1 cells were cultured in usual α-medium and in contact with CaTiO 3 -aC and HA during previous studies 22) . The increased mineralized areas observed in cultures with higher amounts of CaCO 3 might be caused by increased amounts of Ca 2+ released to culture medium.…”

“…Results revealed that the presence of CaCO 3 in CaTiO 3 increases proliferation, diff erentiation and mineralization of bone marrow stromal cells in comparison to commercial CaTiO 3 and hydroxyapatite. Our results thus proved CaTiO 3 -CaCO 3 composites as promising compounds for bone induction in medical and dental fi elds 22) . Hence, the aim of this work is to evaluate the eff ect of diff erent amounts of CaCO 3 combined with CaTiO 3 on the osteoblastic response of KUSA/A1 cells.…”

“…Thus, to determine CaTiO 3 -CaCO 3 effect on KUSA/A1 response, the cells were cultured in α-MEM together with the different compounds synthesized. In previous work we observed that the maximum ALP activity increased at day 7 in CaTiO 3 -CaCO 3 sintered at 650ºC for 2 h compared to CaTiO 3 -aC, CaTiO 3 and HA indicating its benefi cial eff ect for osteoblastic diff erentiation 22) . Moreover, the expression of osteogenic genes was higher in poly(ε-caprolactone)calcium titanate systems in comparison to poly(ε-caprolactone)-strontium titanate and poly(ε-caprolactone)-barium titanate composites when using perovskite ceramic nanoparticles as fi llers in polymer scaff olds, as MCGEE-RUSSELL reported 31) .…”

Enhancement of Photoluminescence and Color Purity of CaTiO3:Eu Phosphor by Li Doping

“…The FTIR spectra were used to obtain the phonon energy of vibrational modes in the host. The phonon energies at 577 and 442 cm −1 are attributed to the characteristics of alkaline titanates and the Ti–O bond vibration of (TiO 3 ) 2− groups, respectively . After Li + doping, higher energy phonons appear at 716 cm −1 .…”